Gas Chromatographic Separation of L-Idonate and D-Gluconate

Chen, Chau-Yang; Imanari, Toshio; Yamamoto, Hirokazu; Kwan, Takao

doi:10.1248/cpb.16.755

Cited by 4 publications

(1 citation statement)

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“…9 CE is a developing separation technique, which has many advantages, such as high efficiency, high rapidity, low cost and low pollution. [9][10][11][12] The conditions for the separation are as follows: a running buffer of 20 mmol L -1 HAc + 6 mmol L -1 NaAc + 12 mg L -1 hydroxypropyl methyl cellulose (HPMC) + 35 mmol L -1 b-CD, a separation voltage of 13 kV, an electrokinetic injection of 11 kV × 10 s. The separation could be completed with 19 min. 9 In 1990, Manz and Widmer et al proposed the multidisciplinary field of microfluidic chip, micro-total analysis systems (m-TAS) or lab-on-chip (LOC) at first.…”

Section: Introductionmentioning

confidence: 99%

Chiral Separation of Ofloxacin Enantiomers by Microchip Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection

Chen

You

Xie

et al. 2014

J Chinese Chemical Soc

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We describe a chiral separation method for ofloxacin enantiomers, levofloxacin and dextrofloxacin by microchip capillary electrophoresis with capacitively coupled contactless conductivity detection. The running buffer included 1 mmol L -1 MES and 1 mmol L -1 Tris (pH 8.0) with a separation voltage of 1.5 kV and an injection time of 10s. Under these conditions, the enantiomers were completely separated within 1 min. The linear calibration curves were A = 5.76 c -0.00587 for levofloxacin and A = 5.41 c -0.00551 for dextrofloxacin, in which the linear concentration of the components all ranged from 0.05 to 0.15 mg mL -1 (regression coefficients were both 0.9996). The limits of detection (S/N = 3) were, respectively, 18 and 21 mg mL -1 . The relative standard deviations of migration time were both 2.0% (n = 6). The relative standard deviations of peak area were 3.4% (n = 6) for levofloxacin and 4.0% (n = 6) for dextrofloxacin. The effects of some factors on resolutions, such as separation voltage and injection time, concentration of running buffers, were studied. The method was simple, rapid, high-efficient. Furthermore, the method could be applied to the chiral separation of the product containing these enantiomers, such as Ofloxacin Eye Drops.

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Section: Introductionmentioning

confidence: 99%

Chiral Separation of Ofloxacin Enantiomers by Microchip Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection

Chen

You

Xie

et al. 2014

J Chinese Chemical Soc

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New mechanisms for the biosynthesis and metabolism of 2‐keto‐L‐gulonic acid in bacteria

Makover

Ramsey

Vane

et al. 1975

Biotech & Bioengineering

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L-Sorbose is oxidized to 2-keto-L-gulonic acid (KGA) via the following sequence of reactions which we call the "sorbosone pathway": L-sorbose in equilibrium L-sorbosone leads to KGA. The first step is reversible and is mediated by enzymes found in a soluble fraction obtained from Pseudomonas putida ATCC 21812. Although no cofactor requirements were found for the forward reaction, the reverse reaction clearly required NADH. Enzymes for this NADH-dependent synthesis of L-sorbose could be differentiated on the basis of molecular weights. The second step in the sorbosone pathway is catalyzed by a particulate enzyme found in extracts from P. putida and Gluconobacter melanogenus IFO 3293. The rate limiting reaction in the sorbosone pathway is the synthesis of L-sorbosone. In addition to P. putida, Klebsiella pneumoniae (ATCC 27858) and Serratia marcescens (ATCC 27857) also contain the enzymes which catalyze the reactions of the sorbosone pathway. Two of the bacteria studied, P. putida and G. melanogenus, also contain an enzyme involved in the further metabolism of KGA to L-idonic acid. This enzyme, referred to as KGA-reductase, is found in the soluble fraction of cell-free extracts and is dependent on NADH or NADPH.

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Synthesis of L-Ascorbic Acid

Crawford

1980

Advances in Carbohydrate Chemistry and Biochemistry

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Gas Chromatographic Separation of L-Idonate and D-Gluconate

Cited by 4 publications

References 0 publications

Chiral Separation of Ofloxacin Enantiomers by Microchip Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection

Chiral Separation of Ofloxacin Enantiomers by Microchip Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection

New mechanisms for the biosynthesis and metabolism of 2‐keto‐L‐gulonic acid in bacteria

Synthesis of L-Ascorbic Acid

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