Gas chromatographic–mass spectrometric method for the determination of alkylresorcinols in human plasma

Linko, Anna-Maria; Parikka, Kirsti; Wähälä, Kristiina; Adlercreutz, Herman

doi:10.1016/s0003-2697(02)00226-9

Cited by 70 publications

(70 citation statements)

References 14 publications

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“…ARs are mainly consumed via products containing WG wheat or rye or bran from these cereals. Because they are absorbed and can be measured in plasma (3,4), they have been proposed as biomarkers of WG wheat and rye intakes (5,6). As biomarkers of WG wheat and rye intakes, they may be able to categorize subjects as low or high consumers of WG foods, particularly in epidemiologic studies (7) and as a biomarker of compliance with a WG diet in intervention studies (5,8,9).…”

Section: Arsmentioning

confidence: 99%

Plasma alkylresorcinols as a biomarker of whole-grain food consumption in a large population: results from the WHOLEheart Intervention Study

Ross¹,

Bourgeois²,

Macharia³

et al. 2012

The American Journal of Clinical Nutrition

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Background: Small-scale, short-term intervention studies have suggested that plasma alkylresorcinol (AR) concentrations may be biomarkers of whole grain (WG) wheat and rye intakes. Objective: The objective was to determine whether plasma AR concentrations reflect self-reported WG food intake in a 16-wk WG intervention study and to establish which phenotypic characteristics influence plasma AR concentrations. Design: In a randomized parallel-group dietary intervention study, 316 overweight and obese participants with a WG intake of ,30 g/d were recruited and randomly assigned to 1 of 3 groups: control (no dietary change), intervention 1 (60 g WG/d for 16 wk), or intervention 2 (60 g WG/d for 8 wk followed by 120 g WG/d for 8 wk). Fasting blood samples were collected at baseline, 8 wk, and 16 wk for the measurement of plasma lipids and ARs. Results: Plasma samples from 266 study completers were analyzed. Total plasma AR concentrations increased with the WG intervention and could be used to distinguish between control subjects and those who consumed 60 or 120 g WG, but not between those who consumed 60 and 120 g WG. Plasma AR concentrations were higher in men, were positively associated with plasma triglyceride concentrations, and were negatively associated with nonesterified fatty acids. Conclusions: Plasma AR concentrations were correlated with WG intake and could be used to distinguish between low-and high-WG consumers. Sex and plasma lipid concentrations independently influenced plasma AR concentrations, although plasma triglycerides may explain higher concentrations in men. This trial is registered as ISRCT no. 83078872.Am J Clin Nutr 2012;95:204-11.

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Section: Arsmentioning

confidence: 99%

Plasma alkylresorcinols as a biomarker of whole-grain food consumption in a large population: results from the WHOLEheart Intervention Study

Ross¹,

Bourgeois²,

Macharia³

et al. 2012

The American Journal of Clinical Nutrition

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“…Partly owing to a lack of reliable biomarkers, investigation of the association of whole-grain intake with disease prevalence has been difficult. Alkylresorcinols (ARs) are found in high concentrations in rye and wheat whole-grain cereals and have been proposed to function as plasma markers of wholegrain intake (3)(4)(5). ARs are absorbed via the lymphatic system and have a rather short elimination half-life (6,7 ).…”

confidence: 99%

Quantification of Alkylresorcinol Metabolites in Plasma by High-Performance Liquid Chromatography with Coulometric Electrode Array Detection

Koskela

Samaletdin

Aubertin‐Leheudre

et al. 2008

J. Agric. Food Chem.

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Background: Whole-grain rye and wheat cereals contain high amounts of alkylresorcinols (ARs), phenolic lipids. ARs can be quantified in plasma. Two recently identified urinary AR metabolites, 3,5-dihydroxyphenylbenzoic acid (DHBA) and 3-(3,5-dihydroxyphenyl)-1-propanoic acid (DHPPA), may be useful as biomarkers of intake of whole-grain rye and wheat. Methods: We evaluated 4 pretreatment protocols for quantifying urinary DHBA and DHPPA using HPLC coupled with a coulometric electrode array detector. Syringic acid was used as the internal calibrator. Results: Measured urinary concentrations of DHBA and DHPPA were 0.8 -115 mol/L. The mean recoveries of all added concentrations were 85%-104% for DHBA and 86%-99% for DHPPA, depending on the degree of the purification. The protocol versions with less purification correlated well with the protocol including highest purification. The correlation coefficients (r 2 ) were 0.9699 -0.8153 for DHBA and 0.9854 -0.8371 for DHPPA. Conclusion: Although the protocol with the most purification steps was most specific, all protocols were suitable for measuring DHBA and DHPPA in urine. The rapid protocol with simple hydrolysis could be used in large-scale clinical studies. Additional investigation is needed to clarify whether these metabolites are useful biomarkers of whole-grain intake and helpful in the exploration of its association with human diseases.

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“…It has been proposed that ARs could function as biomarkers of human whole grain intake (1,4). In human subjects, ARs have been detected in intact form in plasma (4) and as metabolites [3,5-dihydroxybenozoic acid (DHBA) and 3-(3,5-dihydroxyphenyl)-1-propanoic acid (DHPPA)] in urine (5,6) and plasma. 1 Alkylresorcinols are stable during food processing (7).…”

Section: Introductionmentioning

confidence: 99%

Plasma Alkylresorcinols and Urinary Alkylresorcinol Metabolites as Biomarkers of Cereal Fiber Intake in Finnish Women

Aubertin‐Leheudre

Koskela²,

Marjamaa³

et al. 2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Alkylresorcinols (AR) could be good biomarkers of consumption of fiber-rich cereal products. The aim of this study was to examine the relationship between plasma ARs or urinary AR metabolites and cereal fiber intake in women consuming their habitual diet. Twenty-five postmenopausal and 31 premenopausal women were recruited. The subjects included also vegetarians (n = 20) to obtain a broad range of cereal intake. Dietary intake, plasma ARs, and urinary AR metabolites [3,5-dihydroxybenzoic acid and 3-(3,5-dihydroxyphenyl)-1-propanoic acid] were measured. Pearson's and Partial correlation tests were done between dietary fiber intake and plasma ARs or urinary AR metabolites. Cereal fiber intake correlated significantly with plasma AR C17:0 (r = 0.387), AR C19:0 (r = 0.350), AR C21:0 (r = 0.428), AR C23:0 (r = 0.409), AR C25:0 (r = 0.283), and total AR (r = 0.406) and with urinary AR metabolites DHBA (r = 0.359) and DHPPA (r = 0.402) even after adjustment for body mass index and age, which could be confounding variables. This is the first study to show a significant correlation between plasma ARs or urinary AR metabolites and cereal fiber intake during consumption of a habitual diet. These results indicate that assay of plasma ARs or urinary AR metabolites may be used as biomarkers in epidemiologic studies in free-living populations to evaluate the role of cereal fiber intake in various diseases. (Cancer Epidemiol Biomarkers Prev 2008;17(9):2244 -8)

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Gas chromatographic–mass spectrometric method for the determination of alkylresorcinols in human plasma

Cited by 70 publications

References 14 publications

Plasma alkylresorcinols as a biomarker of whole-grain food consumption in a large population: results from the WHOLEheart Intervention Study

Plasma alkylresorcinols as a biomarker of whole-grain food consumption in a large population: results from the WHOLEheart Intervention Study

Quantification of Alkylresorcinol Metabolites in Plasma by High-Performance Liquid Chromatography with Coulometric Electrode Array Detection

Plasma Alkylresorcinols and Urinary Alkylresorcinol Metabolites as Biomarkers of Cereal Fiber Intake in Finnish Women

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