Gas chromatographic–mass spectrometric determination of 4-nonylphenols and 4-tert-octylphenol in biological samples

Tsuda, Taizo; Takino, Akihiko; Kojima, Mihoko; Harada, Hiroyuki; Muraki, Kazue

doi:10.1016/s0378-4347(98)00524-6

Cited by 46 publications

(27 citation statements)

References 12 publications

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“…As seen from these data, NP9-EDF resulted in a broader mP range for all the antisera tested. Comparison of anti-NP7 (rabbits 1 and 2) and anti-NP9 (rabbits [3][4][5][6][7][8] illustrates the different immune response of individual animals and enables selection of the best antisera, providing maximum inhibition of bound tracer signal. On the basis of optimization of immunoreagent structure by the span analysis, NP9-EDF was chosen as a labeled competitor for further experiments; rabbits 2 and 5 were found to result in the broadest signal span among all antisera against NP7 and NPFA, respectively.…”

Section: Assay Sensitivitymentioning

confidence: 99%

“…AP are stable degradation products of the widely used group of non-ionic surfactants, alkylphenolethoxylates (APEO), and are capable of accumulation in water reservoirs and aquatic organisms. For instance, surveys of AP in environmental samples performed in many countries revealed the presence of NP at concentrations of 10-220 µg L -1 in river water [3,4] and 20-30 µg kg -1 in fish tissues [5,6]. Toxicity, genotoxicity and estrogenic potency data for AP are scarce in the literature, and very much dependent on the type of organism used to evaluate the possible biological effects of these compounds.…”

Section: Introductionmentioning

confidence: 99%

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Express detection of nonylphenol in water samples by fluorescence polarization immunoassay

et al. 2003

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The development of express method for detection of endocrine-disrupting chemicals (EDC) such as alkylphenols is required for ecological monitoring. Several attempts have been made to produce antibodies against 4-nonylphenol (NP) in recent years. This work describes the production of new antibodies against NP and also summarizes the characterization of antibodies obtained earlier. Three approaches used to produce alkylphenol-specific antibodies are compared; these are based on: 1. omega-(4-hydroxyphenyl)nonanoic or omega-(4-hydroxyphenyl)heptanoic acid NP derivatives designed to mimic the linear NP isomer; 2. 4-aminophenol, which potentially mimics various substituted phenolic compounds with different side-chain structures at position 4 of the benzene ring; and 3. a mixture of branched NP isomers, conjugated to the carrier protein via a benzene ring by the Mannich reaction, and expected to be the closest mimic of NP structure by preserving its natural alkyl moiety.Fluorescence polarization immunoassays based on different combinations of antibody and labeled antigen for screening detection of NP were developed and structural aspects of assay sensitivity and specificity were investigated. The assays based on the antisera raised against omega-(4-hydroxyphenyl)nonanoic acid and NP conjugate via Mannich reaction are capable of express detection of NP with detection limit of 7 microg mL(-1 )and assay dynamic range of 18-300 microg mL(-1).

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Section: Assay Sensitivitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Express detection of nonylphenol in water samples by fluorescence polarization immunoassay

et al. 2003

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“…Quantification was accomplished via a fortified calibration curve in matrix; the correlation coefficient was 0.99 and the level of detection 0.010 mg/kg. 8 For octylphenol and nonylphenol acetonitrile was used for extraction and sample clean up performed on a florisil and a C18 cartridge (Tsuda et al, 1999). Analytes are eluted with methanol from C18 and quantification accomplished via a fortified calibration curve.…”

Section: Target Chemical Analysesmentioning

confidence: 99%

Testicular microlithiasis and neoplastic lesions in wild eland (Tragelaphus oryx): Possible effects of exposure to environmental pollutants?

Bornman

Barnhoorn

Jager

et al. 2010

Environmental Research

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The purpose of the study was to compare wildlife in the proximity and away from the sources of known industrial pollution. Macroscopic, focal, gritty areas that appeared white were showed that 11 out of 17 RNR eland contained a detectable estrogenic chemical pnonylphenol (mean ± SD: 184.8 ± 24.6 µg/kg fat); no organochlorine chemicals or polychlorinated biphenyls were detected. Of the 7 SNR eland, 5 had detectable octylphenol residues (50.2 ± 30.9 µg/kg fat), 3 had detectable p-nonylphenol (137.8 ± 77.9 µg/kg fat), 3had o-p'-DDT (114.9 ± 31.1 µg/kg fat), 3 had p-p'-DDT (127.3 ± 49.9 µg/kg(79.5 ± 30.4 µg/kg fat) and 5 contained o-p'-DDE (27.7 ± 9.9 µg/kg fat). One eland from the MNR contained one 70.6 µg o-p'-DDT/kg fat and another p-p'-DDE 61.3 µg/kg fat. Therefore, in eland with testicular abnormalities, significant amounts of various estrogenic chemicals were bioaccumulated in fat samples. It therefore seems likely that the lesions found in RNR and SNR were associated with the relatively high body-burden of environmental pollutants (phenols), although the possibility of systemic infections cannot be ruled out. No testicular 3 abnormalities were found in reference eland. These findings are the first indication of mammalian wildlife being affected by environmental pollution of endocrine disrupting chemicals in South Africa.

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“…The liver extracts were cleaned in accordance with the method of Tsuda et al [19], with minor modifications. The dry extract was dissolved in n-hexane (10 mL) and shaken with acetonitrile saturated with hexane (3 x 30 mL).…”

Section: Clean-up Of Liver Extractsmentioning

confidence: 99%

Accelerated solvent extraction then liquid chromatography coupled with mass spectrometry for determination of 4-t-octylphenol, 4-nonylphenols, and bisphenol A in fish liver

2002

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SummaryAnalysis of extracts from fish liver containing alkylphenol contaminants can be hindered by the presence of co-extracted fats and proteins that interfere with chromatographic analysis. In this study accelerated solvent extraction (ASE), Florisil clean-up, then combined liquid chromatography -mass spectrometry (LC-MS) with an electrospray (ESI) interface have been used to optimize an analytical procedure for the analysis of octylphenol, nonylphenol, and bisphenol A in fish liver. After comparison of the efficiency of ASE with conventional Soxhlet extraction the developed procedure was applied to the analysis of liver samples. Calibration plots of the relationship between concentration and the ratio of the responses to the analyte and to the internal standard, 4-n-nonylphenol, were determined by linear regression analysis over the concentration range 0.05 to 10 ppm and resulted in good fits (r 2 > 0.994). Recoveries (evaluated for each liver sample as the ratio between response to the surrogate compound, 4-n-nonylphenol, and that to the internal standard, 4-n-heptylphenol, relative to the same ratio for a reference standard solution) were 53 • 20%.Under the experimental conditions used in this work the limits of detection (LOD), calculated by use of a signal-to-noise ratio of 3:1, were 5 ng g 1 for4-t-odylphenol, 15 ng g 1 for bisphenol A, and 20 ng g 1 for nonylphenol. The method can be satisfactorily applied to screening analysis of odyl-and no@phenols and bisphenol A in biological samples such as fish liver.

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Gas chromatographic–mass spectrometric determination of 4-nonylphenols and 4-tert-octylphenol in biological samples

Cited by 46 publications

References 12 publications

Express detection of nonylphenol in water samples by fluorescence polarization immunoassay

Express detection of nonylphenol in water samples by fluorescence polarization immunoassay

Testicular microlithiasis and neoplastic lesions in wild eland (Tragelaphus oryx): Possible effects of exposure to environmental pollutants?

Accelerated solvent extraction then liquid chromatography coupled with mass spectrometry for determination of 4-t-octylphenol, 4-nonylphenols, and bisphenol A in fish liver

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