Gas chromatographic–mass spectrometric analysis of urinary sugar and sugar alcohols during pregnancy

Tetsuo, M.; Zhang, Chunhua; Matsumoto, Hirofumi; Matsumoto, Isamu

doi:10.1016/s0378-4347(99)00209-1

Cited by 28 publications

(14 citation statements)

References 20 publications

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“…GC analysis of the trimethylsilyl ether derivatives of sugars is a well-known procedure [9], as is the GC separation of sugars as alditol acetates [7]. However, sample preparation for GC is extensive, involving the derivatization of sugars with specialty reagents to render them more volatile [10]. Also, the sensitive chromatographic operating parameters for GC are not well suited for routine analysis.…”

mentioning

confidence: 99%

Multiple-reaction monitoring liquid chromatography mass spectrometry for monosaccharide compositional analysis of glycoproteins

Hammad

Saleh

Novotný

et al. 2009

J. Am. Soc. Mass Spectrom.

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A simple, sensitive, and rapid quantitative LC-MS/MS assay was designed for the simultaneous quantification of free and glycoprotein bound monosaccharides using a multiple reaction monitoring (MRM) approach. This study represents the first example of using LC-MS/MS methods to simultaneously quantify all common glycoprotein monosaccharides, including neutral and acidic monosaccharides. Sialic acids and reduced forms of neutral monosaccharides are efficiently separated using a porous graphitized carbon column. Neutral monosaccharide molecules are detected as their alditol acetate anion adducts [M ϩ CH 3 CO 2 ] Ϫ using electrospray ionization in negative ion MRM mode, while sialic acids are detected as deprotonated ions [M Ϫ H] Ϫ . The new method exhibits very high sensitivity to carbohydrates with limits of detection as low as 1 pg for glucose, galactose, and mannose, and below 10 pg for other monosaccharides. The linearity of the described approach spans over three orders of magnitudes (pg to ng). The method effectively quantified monosaccharides originating from as little as 1 g of fetuin, ribonuclease B, peroxidase, and ␣ 1 -acid glycoprotein human (AGP) with results consistent with literature values and with independent CE-LIF measurements. The method is robust, rapid, and highly sensitive. arbohydrates play vital roles in the control of many key biological processes by acting as reciprocating compounds with proteins in molecular recognition events [1,2]. Associative interactions between oligosaccharides on glycoproteins and lectins on the surfaces of binding partners can be involved in the initiation steps of many diseases such as influenza, cholera, stomach cancer, and cancer metastasis [3]. An understanding of these initiating binding processes at the molecular level requires the structural determination of oligosaccharides and the quantification of the glycan monosaccharide constituents, which would be essential towards the development of possible cures for many diseases [4,5].Monosaccharides compositional analysis is commonly performed using gas-chromatography [6 -10], liquid-chromatography [11-13], or capillary electrophoresis [14 -21]. GC/MS chromatography with an electron-impact ion source has been the traditional method of choice for the quantification of neutral monosaccharides. GC analysis of the trimethylsilyl ether derivatives of sugars is a well-known procedure [9], as is the GC separation of sugars as alditol acetates [7]. However, sample preparation for GC is extensive, involving the derivatization of sugars with specialty reagents to render them more volatile [10]. Also, the sensitive chromatographic operating parameters for GC are not well suited for routine analysis. Liquid chromatography analysis of monosaccharides is complicated by the fact that these compounds do not possess a UV chromophore and consequently, laborious derivatization with chromophores or fluorophores is required before UV or fluorescence detection [22][23][24][25][26]. Neutral sugars have also been analyzed by HPLC using...

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confidence: 99%

Multiple-reaction monitoring liquid chromatography mass spectrometry for monosaccharide compositional analysis of glycoproteins

Hammad

Saleh

Novotný

et al. 2009

J. Am. Soc. Mass Spectrom.

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“…Inexpensive derivatization reagents however, such as acetic anhydride, do not provide optimum volatility for carbohydrates, reducing sensitivity, limiting analysis to abundant species such as glucose. Increased sensitivity of carbohydrate detection by GC/MS has been achieved with specialty reagents for derivatization; however, they are significantly more expensive [8,10,16]. LC/ MS-based methods are potentially advantageous because of the simplification of sample processing [4 -6, 11].…”

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Sensitive LC MS quantitative analysis of carbohydrates by Cs⁺ attachment

Rogatsky

Jayatillake

Goswami

et al. 2005

J. Am. Soc. Mass Spectrom.

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The development of a sensitive assay for the quantitative analysis of carbohydrates from human plasma using LC/MS/MS is described in this paper. After sample preparation, carbohydrates were cationized by Cs ϩ after their separation by normal phase liquid chromatography on an amino based column. Cesium is capable of forming a quasi-molecular ion [M ϩ Cs] ϩ with neutral carbohydrate molecules in the positive ion mode of electrospray ionization mass spectrometry. The mass spectrometer was operated in multiple reaction monitoring mode, and transitions [M ϩ 133] ¡ 133 were monitored (M, carbohydrate molecular weight). The new method is robust, highly sensitive, rapid, and does not require postcolumn addition or derivatization. It is useful in clinical research for measurement of carbohydrate molecules by isotope dilution assay. (J Am Soc Mass Spectrom 2005, 16, 1805-1811

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“…20,21) In the present study, it was found that FJHQ(SR) markedly decreases urinary glucose, sorbitol, fructose, myo-inositol and 1,5-anhydro-Dglucitol in STZ-diabetic mice. This indicates that FJHQ(SR) is effective in suppressing an excessively activated polyol pathway in diabetes, as well as hyperglycemia and reduced insulin deficiency.…”

Section: Discussionmentioning

confidence: 48%

“…Urine samples were prepared using a urease treatment method as follows: 20,21) One hundred µl of urine was incubated with 30 units of urease at 37°C for 30 min. After adding an internal standard (20 µl of n-heptadecanoic acid), the sample was mixed with 900 µl of ethanol and centrifuged to deproteinize it; the supernatant was collected and evaporated.…”

Section: Measurement Of Urinary Sugar and Sugarmentioning

confidence: 99%

“…To address this question, we analyzed the urinary levels of glucose, fructose, myo-inositol and 1,5-anhydro-D-glucitol in STZ-diabetic mice treated with or without FJHQ(SR), by gas chromatography mass spectrometry with some modifications. 20,21) In addition, the effects of FJHQ(SR) was compared to those of FJHQ(SCR), since it is possible that these two FJHQs have different effects on the polyol pathway in diabetes.…”

Section: Introductionmentioning

confidence: 99%

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The Effects of a Traditional Medicine, Fang-ji-huang-qi-tang (Boi-ogi-to), on Urinary Sugar and Sugar Alcohols in Streptozotocin-induced Diabetic Mice.

Liu

Tsutsumi

Zhang³

et al. 2002

JOURNAL OF HEALTH SCIENCE

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clinically used in the treatment of arthritis and edema in both Japan and China. Since FJHQ(SCR) has been reported to inhibit the elevation of serum cholesterol and decrease the ratio of visceral fat to somatic fat in diabetic patients with obesity, 1) it is possible that FJHQ(SR) has a similar or more beneficial effect in improving diabetes.Streptozotocin (STZ)-induced diabetes mellitus has been widely accepted as a model of insulin-dependent diabetes mellitus.2) However, STZ-diabetic rodents still have low insulin levels and survive for some months. Recently, several investigators have reported that a single treatment of low doses of STZ induces a non-insulin-dependent diabetes mellitus (NIDDM)-like hyperglycemic state in both neonatal and adult rodents. [3][4][5] This diabetic model characteristically shows mild hyperglycemia and impaired pancreatic function, accompanied by insulin resistance. In addition, the oral administration of antidiabetic drugs such as tolbutamide and glibenclamide improves hyperglycemia and increases the blood insulin level in STZ-diabetic rodents.6-8) These characteristics of STZ-diabetic mice are very similar to those of NIDDM.The chronic complications of diabetes, including retinopathy, neuropathy and nephropathy, 9) are the major causes of morbidity and mortality associated with the disease. It has been shown that diabetic complications may result from damage of cellular function caused by abnormal polyol metabolism. [10][11][12][13] In other words, the complications in poorly controlled diabetes would be ultimately caused by abnormal polyol metabolism 14) as well as glycation of proteins, 15,16) because the modification of nucleic acids and proteins by polyol can be due to activation of the hyperglycemia-induced polyol pathway. 17)In our previous studies, it was found that high blood glucose is reduced, whereas blood immunoreactive insulin is elevated in STZ-diabetic mice after

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Gas chromatographic–mass spectrometric analysis of urinary sugar and sugar alcohols during pregnancy

Cited by 28 publications

References 20 publications

Multiple-reaction monitoring liquid chromatography mass spectrometry for monosaccharide compositional analysis of glycoproteins

Multiple-reaction monitoring liquid chromatography mass spectrometry for monosaccharide compositional analysis of glycoproteins

Sensitive LC MS quantitative analysis of carbohydrates by Cs⁺ attachment

The Effects of a Traditional Medicine, Fang-ji-huang-qi-tang (Boi-ogi-to), on Urinary Sugar and Sugar Alcohols in Streptozotocin-induced Diabetic Mice.

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Gas chromatographic–mass spectrometric analysis of urinary sugar and sugar alcohols during pregnancy

Cited by 28 publications

References 20 publications

Multiple-reaction monitoring liquid chromatography mass spectrometry for monosaccharide compositional analysis of glycoproteins

Multiple-reaction monitoring liquid chromatography mass spectrometry for monosaccharide compositional analysis of glycoproteins

Sensitive LC MS quantitative analysis of carbohydrates by Cs+ attachment

The Effects of a Traditional Medicine, Fang-ji-huang-qi-tang (Boi-ogi-to), on Urinary Sugar and Sugar Alcohols in Streptozotocin-induced Diabetic Mice.

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Sensitive LC MS quantitative analysis of carbohydrates by Cs⁺ attachment