Gas chromatographic determination of meprobamate in serum or plasma after solid-phase extraction

Gaillard, Yvan; Gay-Montchamp, J. P.; Ollagnier, M.

doi:10.1016/0378-4347(92)80615-w

Cited by 13 publications

(4 citation statements)

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“…Most of these methods use CPG [5][6][7][8][9][10][11][12][13][14][15] or HPLC-UV [4]. All of them required solid or liquid phase extraction and some of them derivatization of meprobamate to enhance detection.…”

Section: Discussionmentioning

confidence: 98%

“…Several methods have been reported for the quantification of meprobamate in human plasma, high-performance liquid chromatography [4] and gas chromatography [5][6][7][8][9][10][11][12][13][14][15]. These methods present the following drawbacks: long analysis time and/or complex sample pre-treatment (derivatization, solid or liquid phase extraction).…”

Section: Introductionmentioning

confidence: 99%

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HPLC MS/MS method for quantification of meprobamate in human plasma: Application to 24/7 clinical toxicology

Delavenne

Gay-Montchamp

Basset

2011

Journal of Chromatography B

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

HPLC MS/MS method for quantification of meprobamate in human plasma: Application to 24/7 clinical toxicology

Delavenne

Gay-Montchamp

Basset

2011

Journal of Chromatography B

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“…Several chromatographic methods have been reported for analysis of CSP and/or MPB in biological matrices, including high‐performance liquid chromatography (HPLC),6, 7 gas chromatography (GC),8–12 gas chromatography/mass spectrometry (GC/MS),13–18 and liquid chromatography/mass spectrometry (LC/MS) 19. However, none of these reports has included the simultaneous determination of CSP and MPB in human hair.…”

mentioning

confidence: 99%

Simultaneous determination of carisoprodol and meprobamate in human hair using solid‐phase extraction and gas chromatography/mass spectrometry of the trimethylsilyl derivatives

Kim

Paeng

et al. 2005

Rapid Comm Mass Spectrometry

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Carisoprodol (CSP) is a musculoskeletal relaxant whose active metabolite is meprobamate (MPB). This drug has recently been noticed to be abused as an inexpensive alternative to illicit drugs in Korea. A method using solid-phase extraction (SPE) and gas chromatography/mass spectrometry (GC/MS) was developed for the determination of CSP and MPB in human hair. Hair samples (30 mg) were washed with distilled water and acetone, cut into small fragments (<1 mm), incubated in 1.0 M HCl overnight at 50 degrees C, and then adjusted to pH 6.5. The drugs were extracted from the resulting hydrolyzed solutions using a SPE column. The eluents were evaporated to dryness, then derivatized using N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) with 1% trimethylchlorosilane (TMCS) at 120 degrees C for 30 min. The derivatized extract (1 microL) was injected into the GC/MS system. Recoveries were in the range of 91.5-93.1% for CSP and 85.5-93.0% for MPB. The linear ranges were 0.5-10.0 ng/mg for both CSP and MPB with good correlation coefficients (r(2) = 0.995). The intra-day precision and accuracy ranged from 1.5 to 9.3% and -17.5 to 3.6%, respectively, and the inter-day precision and accuracy ranged from 3.9 to 6.2% and -15.0 to -3.9%, respectively. The limits of detection for CSP and MPB were 0.13 and 0.12 ng/mg, respectively. The applicability of the method was proven by analyzing a hair sample from an authentic abuser.

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“…GC after solid-phase extraction has been used to determine meprobamate in serum or plasma (349). Analysis and isolation of indole alkaloids of fungi by HPLC has been reported (350).…”

mentioning

confidence: 99%

Forensic science

Brettell¹,

Saferstein²

1993

Anal. Chem.

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Gas chromatographic determination of meprobamate in serum or plasma after solid-phase extraction

Cited by 13 publications

References 4 publications

HPLC MS/MS method for quantification of meprobamate in human plasma: Application to 24/7 clinical toxicology

HPLC MS/MS method for quantification of meprobamate in human plasma: Application to 24/7 clinical toxicology

Simultaneous determination of carisoprodol and meprobamate in human hair using solid‐phase extraction and gas chromatography/mass spectrometry of the trimethylsilyl derivatives

Forensic science

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