Gas chromatographic analysis of methanol and ethanol in marine sediment pore waters: Validation and implementation of three pretreatment techniques

Zhuang, Guang‐Chao; Lin, Yu‐Shih; Elvert, Marcus; Heuer, Verena B; Hinrichs, Kai‐Uwe

doi:10.1016/j.marchem.2014.01.011

Cited by 41 publications

(38 citation statements)

References 32 publications

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“…Due to the high concentrations and production rates, methylamines might be the key metabolites fueling methanogenesis, particularly in salt marsh or hypersaline sediment (King ; McGenity ). In spite of the high turnover rate, the importance of methanol or methylamine for methane production requires further investigation, as the in situ abundance of those compounds and their precursors remains largely unconstrained in marine sediments (Wang and Lee ; Zhuang et al ; Zhuang et al ).…”

Section: Discussionmentioning

confidence: 99%

“…Acetate was measured after derivatization using high‐performance liquid chromatography (HPLC) (Albert and Martens ); standards were prepared using reagent grade sodium acetate trihydrate. Methanol was determined by a modified purge and trap pretreatment system (original method from Zhuang et al ) connected to a GC–FID (SRI) with an MXT ® ‐Q‐PLOT column (30 m × 0.52 mm I.D., Restek); the method was standardized using analytical grade methanol (Sigma‐Aldrich). Quantification of DOC was achieved using high‐temperature catalytic oxidation with a Shimadzu TOCV, according to the procedures described in Joye et al ().…”

Section: Methodsmentioning

confidence: 99%

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Effects of pressure, methane concentration, sulfate reduction activity, and temperature on methane production in surface sediments of the Gulf of Mexico

Zhuang

Montgomery

Sibert

et al. 2018

Limnology & Oceanography

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Microbial methanogenesis is a known source of methane in marine environment, but the factors affecting the production of methane from different pathways remain largely unconstrained. We investigated the effects of pressure, methane concentration, temperature, and sulfate reduction activity on the conversion of methanogenic substrates to methane using radiotracers in nearshore and offshore surface sediments in the northern Gulf of Mexico. The transformation of bicarbonate to methane increased with methane concentrations under quasi in situ conditions of deep‐sea sediment, potentially reflecting, to some degree, enhancement of the enzymatic back reaction under high‐methane conditions. In coastal sediments, a positive effect of both increased methane concentration and inhibition of sulfate reduction by molybdate on 14CH4 accumulation suggests that anaerobic oxidation of methane and methanogenesis occur concurrently. In contrast, methane production from methanol was suppressed by increased methane concentration likely due to the reduced energy yield of methanogenesis at elevated methane concentrations. Temperature could limit hydrogenotrophic and acetoclastic methanogenesis under in situ conditions, as higher rates were observed at 40°C in nearshore sediments. Inhibitor experiments with 2‐bromoethanesulfonate and molybdate indicated that sulfate‐reducing bacteria preferentially utilized acetate, as well as H2. Methanol was a noncompetitive substrate for methanogens in the deep‐sea sediments but was competitively cycled in coastal sediments. An authentic noncompetitive substrate, methylamine, was channeled predominantly to methane production under all conditions tested. The different response of methanogenic activity to substrate availability, metabolic competition, temperature, and pressure between sites suggest variable environmental controls on the methane production in coastal vs. deep‐sea surface sediments.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effects of pressure, methane concentration, sulfate reduction activity, and temperature on methane production in surface sediments of the Gulf of Mexico

Zhuang

Montgomery

Sibert

et al. 2018

Limnology & Oceanography

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“…Likewise, the new capacities offered in the "omics" era for elucidation of taxonomic composition (e.g., Inagaki et al, 2006;Sogin et al, 2006), metabolic activity, and function at single-cell to IODP Proceedings 3 V o l u m e 3 7 0 community levels (e.g., Biddle et al, 2008;Lloyd et al, 2013;Orsi et al, 2013), as well as new molecular-isotopic techniques that link biomass to substrate pools (e.g., Biddle et al, 2006;Morono et al, 2011;Wegener et al, 2012) and central metabolic intermediates to distinct geomicrobiological processes (e.g., Heuer et al, 2006Heuer et al, , 2009Zhuang et al, 2014;Wang et al, 2015), allow entirely new approaches to study microbial life close to the limit of the deep biosphere. Recent advances in analytical capabilities provide a tool kit for rigorously examining the unique geosphere-biosphere interactions in geothermally heated sediment that involve the release of microbially utilizable substrates by thermal decomposition of refractory kerogens that otherwise appear resistant to microbial consumption.…”

Section: Introductionmentioning

confidence: 99%

Untitled

2017

Proceedings of the International Ocean Discovery Program

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International Ocean Discovery Program (IODP) Expedition 370 explored the limits of the biosphere in the deep subseafloor where temperature exceeds the known temperature maximum of microbial life (~120°C) at the sediment/basement interface ~1.2 km below the seafloor. Site C0023 is located in the protothrust zone in the Nankai Trough off Cape Muroto at a water depth of 4776 m, in the vicinity of Ocean Drilling Program (ODP) Sites 808 and 1174. In 2000, ODP Leg 190 revealed the presence of microbial cells at Site 1174 to a depth of ~600 meters below seafloor (mbsf ), which corresponds to an estimated temperature of ~70°C, and reliably identified a single zone of elevated cell concentrations just above the décollement at around 800 mbsf, where temperature presumably reached 90°C; no cell count data was reported for other sediment layers in the 70°-120°C range because the detection limit of manual cell counting for low-biomass samples was not low enough. With the establishment of Site C0023, we aimed to detect and investigate the presence or absence of life and biological processes at the biotic-abiotic transition utilizing unprecedented analytical sensitivity and precision. Expedition 370 was the first expedition dedicated to subseafloor microbiology that achieved time-critical processing and analyses of deep biosphere samples, conducting simultaneous shipboard and shore-based investigations.Our primary objective during Expedition 370 was to study the relationship between the deep subseafloor biosphere and temperature. We comprehensively studied the controls on biomass, activity, and diversity of microbial communities in a subseafloor environment where temperatures increase from ~2°C at the seafloor tõ 120°C and thus likely encompasses the biotic-abiotic transition zone. This included investigating whether the supply of fluids containing thermogenic and/or geogenic nutrient and energy substrates may support subseafloor microbial communities in the Nankai accretionary complex. To address these primary scientific objectives and questions, we penetrated 1180 m and recovered 112 cores of sediment and across the sediment/basalt interface. More than 13,000 samples were collected, and selected samples were transferred to the Kochi Core Center by helicopter for simultaneous microbiological sampling and analysis in laboratories with a superclean environment. In addition to microbiological measurements, we determined the geochemical, geophysical, and hydrogeological characteristics of the sediment and the underlying basement and installed a 13-thermistor sensor borehole temperature observatory into the borehole at Site C0023.

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“…Likewise, the new capacities offered in the "omics" era for elucidation of taxonomic composition (e.g., Inagaki et al, 2006;Sogin et al, 2006), metabolic activity, and function at single-cell to community levels (e.g., Biddle et al, 2008;Lloyd et al, 2013;Orsi et al, 2013), as well as new molecularisotopic techniques that link biomass to substrate pools (e.g., Biddle et al, 2006;Morono et al, 2011;Wegener et al, 2012) and central metabolic intermediates to distinct geomicrobiological processes (e.g., Heuer et al, 2006Heuer et al, , 2009Zhuang et al, 2014;Wang et al, 2015), allow entirely new approaches to study microbial life close to the limit of the deep biosphere. Recent advances in analytical capabilities provide a tool kit for rigorously examining the unique geosphere-biosphere interactions in geothermally heated sediment that involve the release of microbially utilizable substrates by thermal decomposition of refractory kerogens that otherwise appear resistant to microbial consumption.…”

Section: Introductionmentioning

confidence: 99%