Garlic and onion oils inhibit proliferation and induce differentiation of HL-60 cells

Seki, Taiichiro; Tsuji, Kentaro; Hayato, Yumi; Moritomo, Tadaaki; Ariga, Toshio

doi:10.1016/s0304-3835(00)00552-8

Cited by 62 publications

(40 citation statements)

References 17 publications

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“…HL-60 differentiation into granulocytic cells was induced by 1.25% (v/v) dimethyl sulfoxide (DMSO), and assayed by nitroblue tetrazolium (NBT) reduction. 19) More than 50% of the cells were NBT-positive after 4 d in culture. The differentiation of K562 towards the erythroid lineage was induced by 30 mM hemin, and 52% of the cells stained positive for benzidine after 4 d in culture.…”

Section: Materials We Purchased [mentioning

confidence: 98%

Expression Profiling of Sulfotransferases in Human Cell Lines Derived from Extra-Hepatic Tissues.

Tamura

Taniguchi

Hayashi

et al. 2001

Biological & Pharmaceutical Bulletin

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Section: Materials We Purchased [mentioning

confidence: 98%

Expression Profiling of Sulfotransferases in Human Cell Lines Derived from Extra-Hepatic Tissues.

Tamura

Taniguchi

Hayashi

et al. 2001

Biological & Pharmaceutical Bulletin

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“…70,71 In one placebo-controlled trial, the application of chloroform extracts of garlic was reported to result in the complete resolution of cutaneous warts with no recurrence after 3-4 months. 72 …”

Section: Garlic Extractsmentioning

confidence: 99%

An Armamentarium of Wart Treatments

Lipke¹

2006

Clinical Medicine & Research

277

295

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Patients and clinicians experience the frustration of cutaneous viral warts caused by infection with the human papilloma virus (HPV).Warts appear in various forms on different sites of the body and include common warts (verruca vulgaris), plane or flat warts, myrmecia, plantar warts, coalesced mosaic warts, filiform warts, periungual warts, anogenital warts (venereal or condyloma acuminata), oral warts and respiratory papillomas. Cervical infection with HPV is now known to cause cervical cancer if untreated. A review of the medical literature reveals a huge armamentarium of wart monotherapies and combination therapies. Official evidence-based guidelines exist for the treatment of warts, but very few of the reported treatments have been tested by rigorous blinded, randomized controlled trials.Therefore, official recommendations do not often include treatments with reportedly high success rates, but they should not be ignored when considering treatment options. It is the purpose of this review to provide a comprehensive overview of the wart treatment literature to expand awareness of the options available to practitioners faced with patients presenting with problematic warts. Warts of the genital tract carry a much more ominous and pernicious threat for women in particular. It has been estimated that up to 70% of sexually active women become infected during their lifetime with human papillomavirus (HPV), the infective agent that causes warts. 3-5 A causal role for HPV infections in cervical cancer has been documented beyond reasonable doubt. HPV DNA is present in virtually all cervical cancer cases worldwide, with it being detected in 99.7% of an international series of cervical cancers by genetic amplification techniques and in 100% of cases as confirmed by histological review. 6,7 Cervical cancer is the most frequent common cancer in developing countries and the second most common cancer in women worldwide. 3 The association between HPV infection and cervical cancer is so strong that HPV is now considered the first positive cause of any human cancer ever identified. 3This raises the level of concerns over HPV prevention, screening and treatment from that of a nuisance condition to that of a major public health concern. Attention is beginning to focus on the viral factors that determine persistence and neoplastic progression to cancer and the possible role of HPV in other nongenital cancers (e.g., skin, upper aerodigestive tract). [8][9][10][11][12][13][14][15][16][17] Patients with warts seek advice from general practitioners, pharmacists, naturopaths, allied health professionals, family or friends, dermatologists, gynecologists, obstetricians and pediatricians for treatment and may present with recalcitrant warts that have been previously treated with anything from folk remedies to hypnosis to over-the-counter medications to more aggressive clinic-based treatments. Unfortunately, even with years of medical literature on this subject, high-quality, level I evidence for the efficacy of almost all treatments is no...

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“…Recently, several lines of investigation have shown that allyl sulfides suppress cell growth and induce apoptosis in multiple cancer cell lines (6 -12). We previously reported that the sulfur-containing volatile oils prepared from garlic and onion inhibit proliferation and induce differentiation of the human promyelocytic leukemia cell line HL-60 (13). However, the molecular mechanisms underlying the antitumorigenesis of allyl sulfides are still not fully understood.…”

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confidence: 99%

Diallyl Trisulfide Suppresses the Proliferation and Induces Apoptosis of Human Colon Cancer Cells through Oxidative Modification of β-Tubulin

Hosono¹,

Fukao²,

Ogihara

et al. 2005

Journal of Biological Chemistry

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217

157

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Allyl sulfides are characteristic flavor components obtained from garlic. These sulfides are thought to be responsible for their epidemiologically proven anticancer effect on garlic eaters. This study was aimed at clarifying the molecular basis of this anticancer effect of garlic by using human colon cancer cell lines HCT-15 and DLD-1. The growth of the cells was significantly suppressed by diallyl trisulfide (DATS, HCT-15 IC 50 ‫؍‬ 11.5 M, DLD-1 IC 50 ‫؍‬ 13.3 M); however, neither diallyl monosulfide nor diallyl disulfide showed such an effect. The proportion of HCT-15 and that of DLD-1 cells residing at the G 1 and S phases were decreased by DATS, and their populations at the G 2 /M phase were markedly increased for up to 12 h. The cells with a sub-G 1 DNA content were increased thereafter. Caspase-3 activity was also dramatically increased by DATS. Fluorescence-activated cell sorter analysis performed on the cells arrested at the G 1 /S boundary revealed cell cycle-dependent induction of apoptosis through the transition of the G 2 /M phase to the G 1 phase by DATS. DATS inhibited tubulin polymerization in an in vitro cell-free system. DATS disrupted microtubule network formation of the cells, and microtubule fragments could be seen at the interphase. Peptide mass mapping by liquid chromatography-tandem mass spectrometry analysis for DATS-treated tubulin demonstrated that there was a specific oxidative modification of cysteine residues Cys-12␤ and Cys-354␤ to form S-allylmercaptocysteine with a peptide mass increase of 72.1 Da. The potent antitumor activity of DATS was also demonstrated in nude mice bearing HCT-15 xenografts. This is the first paper describing intracellular target molecules directly modified by garlic components.Allyl sulfides, e.g. diallyl monosulfide (DAS), 4 diallyl disulfide (DADS), and diallyl trisulfide (DATS), are characteristic flavor components of the essential oil prepared from garlic (Allium sativum L.). Garlic is widely served around the world, and it has been reported that allyl sulfides inhibit both the initiation and promotion stages of tumorigenesis in experimental carcinogenesis models for various types of cancer (1-5). Recently, several lines of investigation have shown that allyl sulfides suppress cell growth and induce apoptosis in multiple cancer cell lines (6 -12). We previously reported that the sulfur-containing volatile oils prepared from garlic and onion inhibit proliferation and induce differentiation of the human promyelocytic leukemia cell line HL-60 (13). However, the molecular mechanisms underlying the antitumorigenesis of allyl sulfides are still not fully understood.Microtubules are ubiquitous proteins present in eukaryotes as components of the cytoskeleton and play pivotal roles in a variety of cellular processes involving cell division, motility, and intracellular trafficking (14). The microtubules are dynamic polymers composed of ␣␤-tubulin heterodimers, and they form the mitotic spindles, which are known to introduce the replicated DNA molecules to the res...

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Garlic and onion oils inhibit proliferation and induce differentiation of HL-60 cells

Cited by 62 publications

References 17 publications

Expression Profiling of Sulfotransferases in Human Cell Lines Derived from Extra-Hepatic Tissues.

Expression Profiling of Sulfotransferases in Human Cell Lines Derived from Extra-Hepatic Tissues.

An Armamentarium of Wart Treatments

Diallyl Trisulfide Suppresses the Proliferation and Induces Apoptosis of Human Colon Cancer Cells through Oxidative Modification of β-Tubulin

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