Gap junctional coupling in the olfactory organ of zebrafish embryos

Weber, Stacy A.; Ross, Linda S.

doi:10.1016/s0165-3806(03)00091-9

Cited by 6 publications

(5 citation statements)

References 29 publications

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“…While such a distribution may simply represent cytosolic or perisomatic localization, it may also represent cell-cell junctions. Interestingly, this Homer expression pattern paralleled the ontogeny of the gap junctional protein connexin in the zebrafish lens, where 36-48 hpf is a critical period for expression (Cheng et al, 2003) and in the olfactory system where 48 hpf was a critical period for the development of functional gap junctions in the developing placode (Weber & Ross, 2003). A similar cell-cell expression pattern was described in Xenopus (Foa et al, 2005), and a cell-cell junction role has been previously described for Homer proteins (Shiraishi et al, 1999).…”

Section: Discussionsupporting

confidence: 71%

Homer 1b/c expression correlates with zebrafish olfactory system development

Gasperini

Foa

2004

J Neurocytol

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The zebrafish, (Danio rerio) is an important model organism for the analysis of molecular mechanisms that govern neuronal circuit development. The neuronal circuitry that mediates olfaction is crucial for the development and survival of all teleost fishes. In concert with other sensory systems, olfaction is functional at early stages in zebrafish development and mediates important behavioral and survival strategies in the developing larva. Odorant cues are transduced by an array of signaling molecules from receptors in olfactory sensory neurons. The scaffolding protein family known as Homer is well placed to orchestrate this signaling cascade by interacting with and coupling membrane bound receptors to cytosolic signaling partners. To date, Homer has not been demonstrated in the zebrafish. Here we report that the Homer 1b/c isoform was prominent in the olfactory system from the earliest stages of differentiation. We describe the spatial and temporal distribution of Homer in the zebrafish olfactory system. At 24 hours post fertilization (hpf), Homer expression delineated the boundary of the presumptive olfactory placode. Subsequent expression steadily increased throughout the developing olfactory placode, with a prominent localization to the dendritic knobs of the olfactory sensory neurons. Homer expression in the developing olfactory bulb was punctate and prominent in the glomeruli, displaying an apparent synaptic localization. This work supports the hypothesis that Homer is an important molecule in neuronal circuit development, necessary for crucial behaviors required for development and survival.

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Section: Discussionsupporting

confidence: 71%

Homer 1b/c expression correlates with zebrafish olfactory system development

Gasperini

Foa

2004

J Neurocytol

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“…Because developing zebrafish have a combination of electrically and chemically coupled neurons, we also tested the effect of inhibiting activity of electrically coupled neurons with carbenoxolone, a gap junction blocker (Rozental et al, 2001; Saint-Amant and Drapeau, 2001; Solomon et al, 2003; Weber and Ross, 2003). We treated olig2:dsred embryos from 30 hpf to 3 dpf and quantified peripheral OPCs at 3 dpf.…”

Section: Resultsmentioning

confidence: 99%

The Neuromodulator Adenosine Regulates Oligodendrocyte Migration at Motor Exit Point Transition Zones

et al. 2019

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SUMMARY During development, oligodendrocyte progenitor cells (OPCs) migrate extensively throughout the spinal cord. However, their migration is restricted at transition zones (TZs). At these specialized locations, unique glial cells in both zebrafish and mice play a role in preventing peripheral OPC migration, but the mechanisms of this regulation are not understood. To elucidate the mechanisms that mediate OPC segregation at motor exit point (MEP) TZs, we performed an unbiased small-molecule screen. Using chemical screening and in vivo imaging, we discovered that inhibition of A2a adenosine receptors (ARs) causes ectopic OPC migration out of the spinal cord. We provide in vivo evidence that neuromodulation, partially mediated by adenosine, influences OPC migration specifically at the MEP TZ. This work opens exciting possibilities for understanding how OPCs reach their final destinations during development and identifies mechanisms that could promote their migration in disease.

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“…1C). Originally the formation of this thickened piece of neurectoderm was thought to result from cell division but there is little cell division until after the formation of the olfactory placode [59] and the cells maintain contact through gap junction [49]. Once this cohesive unit of cells is formed there is an increase in cell division and expression of neurogenic genes [33,34].…”

Section: Appearance (Often Transient) Of a Localized Thickening Of Cellsmentioning

confidence: 99%

Developing a sense of scents: Plasticity in olfactory placode formation

Whitlock

2008

Brain Research Bulletin

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The sense organs of the vertebrate head arise predominantly from sensory placodes. The sensory placodes have traditionally been grouped as structures that share common developmental and evolutionary characteristics. In attempts to build a coherent model for development of all placodes, the fascinating differences that make placodes unique are often overlooked. Here I review olfactory placode development with special attention to the origin and cell movements that generate the olfactory placode, the derivatives of this sensory placode, and the degree to which it shows plasticity during development. Next, through comparison with adenohypophyseal, and lens placodes I suggest we revise our thinking and terminology for these anterior placodes, specifically by: 1) referring to the peripheral olfactory sensory system as neural ectoderm because it expresses the same series of genes involved in neural differentiation and differentiates in tandem with the olfactory bulb, 2) grouping the anterior placodes with their corresponding central nervous system structures and emphasizing patterning mechanisms shared between placodes and these targets. Sensory systems did not arise independent of the central nervous system; they are part of a functional unit composed of peripheral sensory structures and their targets. By expanding our analyses of sensory system development to also include cell movements, gene expression and morphological changes observed in this functional unit, we will better understand the evolution of sensory structures.

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Gap junctional coupling in the olfactory organ of zebrafish embryos

Cited by 6 publications

References 29 publications

Homer 1b/c expression correlates with zebrafish olfactory system development

Homer 1b/c expression correlates with zebrafish olfactory system development

The Neuromodulator Adenosine Regulates Oligodendrocyte Migration at Motor Exit Point Transition Zones

Developing a sense of scents: Plasticity in olfactory placode formation

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