Ganoderma lucidum spore oil induces apoptosis of breast cancer cells in vitro and in vivo by activating caspase-3 and caspase-9

Jiao, Chunwei; Chen, Wang; Tan, Xupeng; Liang, Huijia; Li, Jieyi; Huang, Yun; He, Chunyan; Chen, Jiaming; Ma, Xiaowei; Xie, Yizhen; Yang, Burton B.

doi:10.1016/j.jep.2019.112256

Cited by 114 publications

(73 citation statements)

References 34 publications

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“…Caspase family are usually divided into three protein categories: apoptosis initiators (caspase-9), apoptosis executioners (caspase-3 and − 7) and in ammation mediators [35]. Previous research showed that caspase-3 and caspase-9, are key apoptosis proteins in the apoptosis pathway [36]. The caspase-9 protein is the apoptosis initiator and the apoptosis executors (caspase-3 and − 7) of cell apoptosis in mammals.…”

Section: Discussionmentioning

confidence: 99%

Antitumor Effects of Dihydromyricetin on the Proliferation, Migration and Apoptosis of Human Hepatocellular Carcinoma Hep3B Cells

Jiang

et al. 2021

Preprint

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Background: Hepatocellular carcinoma (HCC) represents a serious public health problem worldwide and has high morbidity and mortality. Dihydromyricetin (DHM) exhibits anti-tumor effect on a variety of malignancies, but its antitumor function of DHM in HCC has been unclear. The aim of this study was designed to investigate the antitumor effect of DHM on cell apoptosis, proliferation, migration and invasion of hepatoma carcinoma cells. Methods: Cultured Hep3B cells were treated with different DHM concentrations, follow by cell apoptosis, proliferation, migration and invasion were examined by CCK-8, colony formation assay, wound healing, Transwell and flow cytometry, respectively. The mRNA and protein expression of apoptosis-associated genes and Bcl-2/Caspase-9 signaling pathway were validated by RT-PCR and western blot. Results: DHM markedly suppressed proliferation, migration, invasion and facilitated apoptosis in Hep3B cells. Mechanistically, DHM significantly downregulated the Bcl-2 expression, and upregulated the mRNA and protein levels of Cleaved-Caspase 3, Cleaved- Caspase 9, Bak, Bax and Bad. Furthermore, in the nude mice tumorigenic model, DHM treatment greatly decreased the weight of the HCC tumors compared to the weights in control and NDP group. Conclusions: DHM could suppress cell proliferation, migration, invasion, and facilitated apoptosis in Hep3B cells. These findings could provide novel insights to develop potential therapeutic strategy for the clinical treatment of HCC.

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Section: Discussionmentioning

confidence: 99%

Antitumor Effects of Dihydromyricetin on the Proliferation, Migration and Apoptosis of Human Hepatocellular Carcinoma Hep3B Cells

Jiang

et al. 2021

Preprint

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“…28,29 G. lucidum is one of the most well-known medicinal mushrooms and has been used for its therapeutic effects for more than 2,000 years. [31][32][33][34] GAs are the most significant secondary metabolites of G. lucidum and have many potential therapeutic applications, as claimed by numerous literature. 35,36 There is a considerable amount of researches that describes the influence of different parameters on growth and GA production of G. lucidum, such as optimization of fermentation conditions, elicitation, and molecular biology technique to achieve higher expression of GA biosynthesis-related genes.…”

Section: T a B L Ementioning

confidence: 99%

“…In the last two decades, numerous studies have explored the active components of G. lucidum and their biological effects along with underlying mechanisms of action 30 . To date, over 300 active compounds have been isolated from G. lucidum with numerous biological activities, including anti‐inflammatory, immune regulation, anti‐tumor, hepatoprotective, hypoglycemic, anti‐aging, neuroprotection, and antioxidant activities 31‐34 . GAs are the most significant secondary metabolites of G. lucidum and have many potential therapeutic applications, as claimed by numerous literature 35,36 .…”

Section: Introductionmentioning

confidence: 99%

Effects of different graphene‐based nanomaterials as elicitors on growth and ganoderic acid production by Ganoderma lucidum

Rostami

Yazdian

Mirjani

et al. 2020

Biotechnology Progress

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Graphene-based nanomaterials (GBNs) have attracted considerable interest nowadays due to their wide range of applications. However, very little attention has been paid to the application of nanomaterials as potential elicitors for production of valuable metabolites. Herein, aiming to earn insight into effects of nanomaterials on secondary metabolite biosynthesis by medicinal fungi, we evaluated the influence of GBNs on growth and production of ganoderic acid (GA) by Ganoderma lucidum in submerged culture. Graphene oxide (GO), reduced graphene oxide (rGO), and rGO/Fe 3 O 4 nanocomposite were synthesized successfully and characterized by X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy analysis. The prepared nanomaterials were added to the culture of G. lucidum at final concentrations of 50, 100, and 150 mg/L on Day 5. The results showed that the elicitation of G. lucidum with GO and rGO decreased the cell dry weight and GA production slightly, especially in higher concentrations. However, rGO/Fe 3 O 4 nanocomposite not negatively affected cell growth and improved GA production. G. lucidum growth rate responded to elicitation experiments differently and depended on the type of nanomaterials and their concentrations, but almost all GBNs caused an increase in GA content (mg/100 mg dry weight). Also, field emission scanning electron microscopy morphological study showed that under elicitation, mycelia were more condensed and tightly stacked together. The findings from this study may suggest that GBNs in low concentrations could be applied as elicitors to secondary metabolites production from higher fungus, but further environmental, physiological, and biological studies required.

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“…Among these proteins, the CASP9 protein acts as an initiator caspase of apoptosis in the mitochondrial cell death pathway. The upregulation of CASP9 can markedly induce the apoptosis of diverse cells [7]. Further studies have emphasized the role of CASP9 in translational medicine in cancer therapy [8].…”

Section: Introductionmentioning

confidence: 99%

CASP9 As a Prognostic Biomarker and Promising Drug Target Plays a Pivotal Role in Inflammatory Breast Cancer

Zhang

Wang

et al. 2021

Preprint

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Background : Inflammatory breast cancer (IBC) is one of the most rare and aggressive subtype of primary breast cancer (BC). Our study aimed to explore hub genes related to the pathogenesis of IBC, which could be considered as novel molecular biomarkers for IBC diagnosis and prognosis. Material and Methods: Two datasets from gene expression omnibus database (GEO) were selected. Enrichment analysis and protein-protein interaction (PPI) network for the DEGs were performed. We analyzed the prognostic values of hub genes in the Kaplan–Meier Plotter. Connectivity Map (CMap) and Comparative Toxicogenomics Database (CTD) was used to find candidate small molecules capable to reverse the gene status of IBC. Results : 157 DEGs were selected in total. We constructed the PPI network with 154 nodes interconnected by 128 interactions. KEGG pathway analysis indicated that the DEGs were enriched in apoptosis, pathways in cancer and insulin signaling pathway. PTEN, PSMF1, PSMC6, AURKB, FZR1, CASP9, CASP6, CASP8, BAD, AKR7A2, ZNF24, SSX2IP, SIGLEC1, MS4A4A and VSIG4 were selected as hub genes based on the high degree of connectivity. Six hub genes (PSMC6, AURKB, CASP9, BAD, ZNF24 and SSX2IP) that were significantly associated with the prognosis of breast cancer. The expression of CASP9 protein was associated with prognosis and immune cells infiltration of breast cance. CASP9- naringenin (NGE) is expected to be the most promising candidate gene-compound interaction for the treatment of IBC. Conclusion : Taken together, CASP9 can be used as a prognostic biomarker and a novel therapeutic target in IBC.

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Ganoderma lucidum spore oil induces apoptosis of breast cancer cells in vitro and in vivo by activating caspase-3 and caspase-9

Cited by 114 publications

References 34 publications

Antitumor Effects of Dihydromyricetin on the Proliferation, Migration and Apoptosis of Human Hepatocellular Carcinoma Hep3B Cells

Antitumor Effects of Dihydromyricetin on the Proliferation, Migration and Apoptosis of Human Hepatocellular Carcinoma Hep3B Cells

Effects of different graphene‐based nanomaterials as elicitors on growth and ganoderic acid production by Ganoderma lucidum

CASP9 As a Prognostic Biomarker and Promising Drug Target Plays a Pivotal Role in Inflammatory Breast Cancer

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