Ganoderma Lucidum Polysaccharides Target a Fas/Caspase Dependent Pathway to Induce Apoptosis in Human Colon Cancer Cells

Liang, Zengenni; Guo, Yutong; Yi, Youjin; Wang, Ren-Cai; Hu, Qiulong; Xiong, Xingyao

doi:10.7314/apjcp.2014.15.9.3981

Cited by 42 publications

(17 citation statements)

References 27 publications

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“…The extracellular death domain reacts with the FADD to form the death induced signal complex, and then activates the caspase-8 as well as the following cascade reaction, thus inducing the apoptosis of cells in presence of Fas protein [23]. Wang et al [24] discussed the HepG-2 proliferation inhibition of Litchi seeds extract and found that the extract L2.3 could stimulate the activity of caspase-8, caspase-3 and caspase-9 and induce the expression of Fas protein (CH3, Figure 1).…”

Section: Regulating the Expression Of Fas Proteinmentioning

confidence: 99%

Research Progress on the Antineoplastic Pharmacological Effects and Mechanisms of Litchi Seeds

Zhang¹,

Zhang²

2015

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Researchers have proven the Litchi seeds of possessing salutary pharmacodynamic effects, such as dispelling cold, relieving pain, promoting the circulation of qi, and removing stasis. This has resulted in its categorization as an affinal drug and diet in the traditional Chinese medicine. Important research progress has been obtained on the chemical components, traditional pharmacological effects, antivirus and antitumor effects, their molecular mechanisms and immune effects of litchi seeds in recent years. In this review, we have focused on the antitumor-related effects and mechanisms for the purpose of better utilization and comprehensive understanding of litchi seeds.

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Section: Regulating the Expression Of Fas Proteinmentioning

confidence: 99%

Research Progress on the Antineoplastic Pharmacological Effects and Mechanisms of Litchi Seeds

Zhang¹,

Zhang²

2015

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“…Recently, many extracts from Chinese medicine have begun to be used as a new strategy for cancer therapy; for example, Ganoderma lucidum polysaccharide and extracts from Aristolochia could induce apoptosis of human colon cancer cells. 6,7 Noticeably, various polyphenolic compounds found in food have been confirmed to have different pharmaceutical functions. 8,9 Previous studies showed that green tea polyphenols could inhibit the growth of breast, lung, and cervical adenocarcinomas by blocking the c-terminal polo-box domain of polo-like kinase 1 10 ; moreover, red wine polyphenol extracts are found to have superior antiproliferative activity in colon cancer.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of growth and migration of oral and cervical cancer cells by citrus polyphenol

Tsai

Hsu

et al. 2016

Journal of the Formosan Medical Association

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“…It is presented in all tissues of the body within the cytoplasm. Once the cells are damaged, LDH was then released into extracellular, the extent of cell damage can be detected by LDH activity [40]. The higher the LDH activity, the more serious the cell injured [41].…”

Section: Discussionmentioning

confidence: 99%

Hederagenin from the leaves of ivy (Hedera helix L.) induces apoptosis in human LoVo colon cells through the mitochondrial pathway

Liu

Zhou

Liu

et al. 2014

BMC Complement Altern Med

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BackgroundColorectal cancer has become one of the leading cause of cancer morbidity and mortality throughout world. Hederagenin, a derivative of oleanolic acid isolated from the leaves of ivy (Hedera helix L.), has been shown to have potential anti-tumor activity. The study was conducted to evaluate whether hederagenin could induce apoptosis of human colon cancer LoVo cells and explore the possible mechanism.MethodsMTT assay was used for evaluating cell viability while Annexin V-FITC/PI assay and Hoechst 33342 nuclear stainining were used for the determination of apoptosis and mitochondrial membrane potential. DCFH-DA fluorescence staining and flow cytometry were used to measure ROS generation. Real-time PCR and western blot analysis were performed for apoptosis-related protein expressions.ResultsMTT assay showed that hederagenin could significantly inhibit the viability of LoVo cells in a concentration-dependent and time-dependent manner by IC50 of 1.39 μM at 24 h and 1.17 μM at 48 h. The apoptosis ratio was significantly increased to 32.46% and 81.78% by the induction of hederagenin (1 and 2 μM) in Annexin V-FITC/PI assay. Hederagenin could also induce the nuclear changes characteristic of apoptosis by Hoechst 33342 nuclear stainining under fluorescence microscopy. DCFH-DA fluorescence staining and flow cytometry showed that hederagenin could increase significantly ROS generation in LoVo cells. Real-time PCR showed that hederagenin induced the up-regulation of Bax and down-regulation of Bcl-2, Bcl-xL and Survivin. Western blotting analysis showed that hederagenin decreased the expressions of apoptosis-associated proteins Bcl-2, procaspase-9, procaspase-3, and polyADP- ribosepolymerase (PARP) were increased, while the expressions of Bax, caspase-3, caspase-9 were increased. However, there was no significant change on caspase-8.ConclusionsThese results indicated that the disruption of mitochondrial membrane potential might contribute to the apoptosis of hederagenin in LoVo cells. Our findings suggested that hederagenin might be a promising therapeutic candidate for human colon cancer.

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Ganoderma Lucidum Polysaccharides Target a Fas/Caspase Dependent Pathway to Induce Apoptosis in Human Colon Cancer Cells

Abstract: Ganoderma lucidum polysaccharides (GLP) extracted from

Cited by 42 publications

References 27 publications

Research Progress on the Antineoplastic Pharmacological Effects and Mechanisms of Litchi Seeds

Research Progress on the Antineoplastic Pharmacological Effects and Mechanisms of Litchi Seeds

Inhibition of growth and migration of oral and cervical cancer cells by citrus polyphenol

Hederagenin from the leaves of ivy (Hedera helix L.) induces apoptosis in human LoVo colon cells through the mitochondrial pathway

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