Gangliosides Protect Bowel in an Infant Model of Necrotizing Enterocolitis by Suppressing Proinflammatory Signals

Schnabl, Kareena; Larsen, Bodil; Aerde, John E. Van; Lees, G.J.; Evans, Mark A.; Belosevic, Miodrag; Field, Catherine J.; Thomson, A. D.; Clandinin, M. T.

doi:10.1097/mpg.0b013e3181b6456d

Cited by 43 publications

(28 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…23,38 In this study, pasteurization did not impact either longchain polyunsaturated fatty acids or gangliosides. Some fatty acid alterations were observed, with pasteurization causing a reduction in relative percentage of oleic acid, and a relative increase in medium-chain fatty acids.…”

Section: Discussionmentioning

confidence: 87%

See 1 more Smart Citation

Effect of pasteurization on selected immune components of donated human breast milk

et al. 2011

View full text Add to dashboard Cite

Objective: Pasteurized, donated milk is increasingly provided to preterm infants in the absence of mother's own milk. The aim of this study was to determine the effect of pasteurization on the concentration of selected components in donated human breast milk.Study Design: Donated milk from 34 mothers was pooled into 17 distinct batches (4 mothers per batch). Aliquots of each batch were then Holder pasteurized (62.5 1C for 30 min). Interferon-g (IFN-g), tumor necrosis factor-a (TNF-a), interleukin-1b (IL-1b), IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p70 and IL-13 were measured in a multiplex enzyme-linked immunosorbent assay (ELISA). Granulocyte colony-stimulating factor (G-CSF), heparin-binding epidermal-like growth factor (HB-EGF) and hepatocyte growth factor (HGF) were measured by ELISA. Lipids were assessed by gas chromatography and gangliosides by the resorcinol-HCl reaction.Result: IFN-g, TNF-a, IL-1b, IL-10 and HGF were significantly reduced by pasteurization (P<0.05). Gangliosides were not affected, but the proportion of medium-chain saturated fats was increased (P<0.05) with a trend towards a decreased proportion of oleic acid (P ¼ 0.057). Conclusion:Pasteurization significantly reduced the concentration of several immunoactive compounds present in breast milk, but did not have an impact on others.

show abstract

Section: Discussionmentioning

confidence: 87%

“…21 Gangliosides and some long-chain polyunsaturated fatty acids are also known to play a role in preventing NEC. 22,23 To date, no investigations on the impact of pasteurization on these immunoactive compounds relevant to NEC have been reported.…”

Section: Introductionmentioning

confidence: 99%

Effect of pasteurization on selected immune components of donated human breast milk

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Dietary ganglioside is able to replace mucosal gangliosides that are continually degraded in inflammatory states. Moreover, increasing ganglioside content through diet decreases proinflammatory cytokine production in intestinal mucosa [24, 28] and prevents hypoxia-induced bowel necrosis and cell injury in cultured infant bowel [29]. The following section summarizes the different modes of action by which dietary gangliosides promote intestinal health.…”

Section: Ganglioside In Dietmentioning

confidence: 99%

“…In preclinical studies, ganglioside treatment increases ganglioside content and inhibits signals caused by proinflammatory stimuli tumour necrosis factor- α and interleukin- (IL-) 1 β in rats [28]. Similarly, ganglioside reduces IL-6 and IL-8 production in cultured infant bowel when exposed to LPS under hypoxic conditions [29]. Replacing ganglioside that is degraded protects the gut by attenuating proinflammatory signals.…”

Section: Mechanisms Of Action Of Gangliosidementioning

confidence: 99%

“…COX and LOX metabolize arachidonic acid (AA) into proinflammatory mediators leukotriene B 4 (LTB 4 ) and prostaglandin E 2 (PGE 2 ). It has been shown that ganglioside prevents production of LTB 4 and PGE 2 in infant bowel when cultured with LPS [29]. Ganglioside appears to inhibit production of LTB 4 and PGE 2 in intestine by blocking nuclear translocation of NF κ B [64].…”

Section: Mechanisms Of Action Of Gangliosidementioning

confidence: 99%

See 1 more Smart Citation

Dietary Ganglioside Reduces Proinflammatory Signaling in the Intestine

Miklavcic

Schnabl²,

Mazurak

et al. 2012

Journal of Nutrition and Metabolism

View full text Add to dashboard Cite

Gangliosides are integral to the structure and function of cell membranes. Ganglioside composition of the intestinal brush border and apical surface of the colon influences numerous cell processes including microbial attachment, cell division, differentiation, and signaling. Accelerated catabolism of ganglioside in intestinal disease results in increased proinflammatory signaling. Restoring proper structure and function to the diseased intestine can resolve inflammation, increase resistance to infection, and improve gut integrity to induce remission of conditions like necrotizing enterocolitis (NEC) and Crohn's disease (CD). Maintaining inactive state of disease may be achieved by reducing the rate that gangliosides are degraded or by increasing intake of dietary ganglioside. Collectively, the studies outlined in this paper indicate that the amount of gangliosides GM3 and GD3 in intestinal mucosa is decreased with inflammation, low level of GM3 is associated with higher production of proinflammatory signals, and ganglioside content of intestinal mucosa can be increased by dietary ganglioside.

show abstract

Lipids in Infant Formula

Zou

Zhang²,

Pedersen

et al. 2020

Bailey's Industrial Oil and Fat Products

View full text Add to dashboard Cite

Human milk (HM) provides the ideal nourishment for infant growth and cognitive development. The new advances in component and microstructure analyses of HM provide solid knowledge for developing better infant formulas to mimic HM to the largest extent possible. In this article, we reviewed the latest progress in the analysis and understanding of the physical structures, composition, and phospholipids and other minor components in HM lipids, as well as in its counterpart infant formula (IF) lipids. The similarities and differences of lipids between HM and IF suggest the gaps to be narrowed for improved IF development, as the theme of IF lipids studies, which are not only on fatty acid composition, but also on triacylglycerol (TAG) profiles and minor lipid species and level of supplements. The efforts and researches in searching alternative oils and fats and development of related chemical/enzymatic processes comprise a key focus of this article. The specifications to regulate IF market, marketing trend, and patenting status are also intensively discussed. Moreover, the risk substance controls of 3‐monochloro‐1,2‐propanediol (3‐MCPD esters) and glycidyl esters (GE) and the strategy to mitigate them during IF lipid process are also reviewed.

show abstract

Gangliosides Protect Bowel in an Infant Model of Necrotizing Enterocolitis by Suppressing Proinflammatory Signals

Abstract: A bowel protective effect of gangliosides is indicated by modulation of vasoactive mediators and proinflammatory signal suppression.

Cited by 43 publications

References 40 publications

Effect of pasteurization on selected immune components of donated human breast milk

Effect of pasteurization on selected immune components of donated human breast milk

Dietary Ganglioside Reduces Proinflammatory Signaling in the Intestine

Lipids in Infant Formula

Contact Info

Product

Resources

About