Gangliosides of bovine adrenal medulla

Ledeen, Robert W.; Salsman, Kenneth; Cabrera, Mirel

doi:10.1021/bi00846a035

Cited by 115 publications

(28 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the ganglioside samples were reacetylated after hydrolysis, it was not possible to determine by GLC whether N-glycolylneuraminic acid was present. However, none was detectable in samples kindly analyzed for us by Dr. Robert Ledeen (19). This confirms the findings of Madoff, Ebbe, and Baldini (35).…”

Section: Resultssupporting

confidence: 87%

“…The basic TLC techniques were those previously described (18) procedure (levised by Ledeen, Salsmani, anid Cabrera (19) w!as used. The solvenit system was chloroform-methaniol-2.5 N ammonium hydroxide (60: 40: 9).…”

Section: Methodsmentioning

confidence: 99%

“…The idenitifie(d gangliosi(le banids were scrape(d fromIl the plate and(I extracte(I tilree timiles with acetone, which removed most of the staini (20). Elution was thlen carried out usinig 5-10 nml of chlor-oformii-nmethaniolwater, 50: 50: 15 (freshly prel)ared) (19), anid this was repeated twice. The combined eluates were taken to dryness and stored in the solicl state at -20°C before the next experimental procedure.…”

Section: Methodsmentioning

confidence: 99%

“…Carbohydrate analyses by GLC were based on methods developed by Sweeley and Walker (22) and by Ledeen and associates (19). All preparative operations were carried out under cover of nitrogen.…”

Section: Gas-liquiid Chromiatography (Glc)mentioning

confidence: 99%

“…After desiccation, trimethylsilyl (TM S) derivatives were prepared using 100-200 ,ul of the reagent described by Carter and Gaver (25). The silvl reagents were evaporated and( the derivatixes taken up in liexane (19) to a finial volume of 25 ul. GLC wxas carried out ioimiiicdiatelv after silylation on a Supelcoport0 column (80/100 mesh) coated x-ith 3%c OV-1.9 The column was previously conditioned for 24-36 hr at 260°C under a nitrogen pressure of 50 psi.…”

Section: Gas-liquiid Chromiatography (Glc)mentioning

confidence: 99%

See 4 more Smart Citations

Studies on Human Platelet Gangliosides

Marcus¹,

Ullman²,

Safier³

1972

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T Gangliosides, glycosphingolipids which contain sialic acid, were studied in human platelets. They represented 0.5% of the platelet lipids and accounted for 6% of the total neuraminic acid content of platelets. Three major ganglioside fractions were identified and characterized. Ganglioside I was hematoside (G6) and comprised 92% of the platelet gangliosides. It contained glucose, galactose, and sialic acid in molar ratios of 1: 1: 1 and no hexosamnine. The imiajor fatty acid was behenate (22: 0). Ganglioside I w-as also identified in isolated platelet granules and membranes. Ganglioside II (5%) contained glucose, galactose. sialic acid, and hexosamines (molar ratios 1: 2: 1: 1). The hexosamines were glucosamine (72%) and galactosamine (28%). It was therefore designated as ganglioside lacto-N-neotetraose. Ganglioside III (2%) contained disialosyllactosyl ceramide (G3A) as well as two other gangliosides which could not be precisely characterized. Gangliosides I, II, and III were susceptible to the action of Clostridiuin perfringens neuraminidase as evidenced by full recovery of sialic acid in its free form after incubation. Neutral platelet glycolipids were qualitatively examined by thinlayer chromatography. The major component was lactosvI ceramide.Interactions of gangliosides I and III and serotonin-'4Cwere examined in an equilibrium dialysis system at 4°C. The gangliosides bound serotonin-'4C in relatively small quantities, wlhereas cointrol lipids w-ere negative. The binding was essentially tinchainged by reverse dialysis, ultracentrifugation anld stubsequen-t tlhin-laver chromatography. The results are comparable to the previously observed nonmetabolic interactions between wlhole platelets and serotonin in the cold. It is suggested that the orientation and specific distribtution of platelet membrane glycolipids may be important determinants of the unique surface properties of platelets.

show abstract

Section: Resultssupporting

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Gas-liquiid Chromiatography (Glc)mentioning

confidence: 99%

Section: Gas-liquiid Chromiatography (Glc)mentioning

confidence: 99%

See 3 more Smart Citations

Studies on Human Platelet Gangliosides

Marcus¹,

Ullman²,

Safier³

1972

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

The Determination of Carbohydrate in Biological Materials by Gas‐Liquid Chromatography

Clamp

Bhatti

Chambers

1971

Methods of Biochemical Analysis

207

View full text Add to dashboard Cite

Morphological changes, cell aggregation and cell membrane alterations caused by phorbol 12,13‐dibutyrate in human blood lymphocytes

Patarroyo

Yogeeswaran

Biberfeld

et al. 1982

Intl Journal of Cancer

View full text Add to dashboard Cite

Nanomolar concentrations of phorbol 12.13-dibutyrate [P(Bu)J were found to affect the morphology of human blood lymphocytes. Changes in the properties and the biochemical composition of the plasma membrane were particularly apparent. Light and electron microscopy (scanning and transmission) showed polarization of the cytoplasm, consistent with uropod formation, in the majority of the cells within 15 min of treatment. Ruffled membranes and cell-to-cell aggregation were observed within 2 h. Larger aggregates, long surface projections and signs of lymphocyte activation (lymphoblasb and mitotic figures) were detected after 24 and 48 h. Cell-tocell aggregation was monitored by light transmission in an aggregometer and confirmed by light and electron microscopy. Aggregation occurred rapidly after the addition of the phorbol ester and was maximal within I h. Cells treated for 24 h formed large clumps. Only 44 % of the cells remained single, compared with 97% in the untreated control. About I 7 % of the treated cells bound autologous untreated lymphocytes, as shown by conjugate formation between fluorescein-labelled P(Bu), treated and untreated unlabelled cells. Synthesis and cell surface exposure of sialyl components were studied in untreated and 48 h P(Bu),-treated cells by metabolic labelling of sialic acid with the sialic-acid-specific precursor N-acetyl-['HI-mannosamine (ManNAc) followed by neuraminidase hydrolysis. Cells treated with P(Bu), for 48 h showed reduced incorporation of ManNAc and a two-to three-fold reduction in neuraminidase-releasable sialic acid, compared with untreated cells. Mild periodate oxidation followed by sodium borotritide labelling showed a 20-30 % reduction in cell surface sialic acid in the phorboltreated cells. The degree of sialylation of galactosyl and Macetyl galactosamine residues in P(Bu),-treated cells, measured by galactose oxidase -sodium borotritide labelling of neuraminidase -treated versus untreated cells, showed a 15-16% reduction compared to controls. Study of glycosphingolipids showed a marked increase of ['H]-galactose incorporation into major glycolipids with chromatographic mobilities of ceramide monohexoside, globoside, G,,, G,, and G,,, in P(Bu), treated lymphocytes. There were no detectable qualitative or quantitative changes in glycoproteins, as judged by surface iodination and borotritide labelling. This suggests that the ganglioside compartment is responsible for the decreased surface sialic acid.

show abstract

Gangliosides of bovine adrenal medulla

Cited by 115 publications

References 36 publications

Studies on Human Platelet Gangliosides

Studies on Human Platelet Gangliosides

The Determination of Carbohydrate in Biological Materials by Gas‐Liquid Chromatography

Morphological changes, cell aggregation and cell membrane alterations caused by phorbol 12,13‐dibutyrate in human blood lymphocytes

Contact Info

Product

Resources

About