Gangliosides in acute myeloid leukaemia (AML) and non-hodgkin's lymphoma (NHL)

Anh-Tuan, Nguyen; Pick, J.; A, Mód; Hollán, Susan R.

doi:10.1016/0277-5379(86)90068-4

Cited by 13 publications

(11 citation statements)

References 10 publications

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“…Aberrant and elevated expression of some gangliosides has been previously demonstrated on the surface of lymphoid derived tumors [24, 25]. In previous studies, GM3 was the predominant ganglioside found in pre-B lymphoma cells and B-cell neoplasms using over-pressured thin-layer chromatography (OPTLC) followed by scanning densitometry and high performance thin layer chromatography (HPTLC) and immune thin layer chromatography (ITLC), respectively.…”

Section: Discussionmentioning

confidence: 99%

“…In previous studies, GM3 was the predominant ganglioside found in pre-B lymphoma cells and B-cell neoplasms using over-pressured thin-layer chromatography (OPTLC) followed by scanning densitometry and high performance thin layer chromatography (HPTLC) and immune thin layer chromatography (ITLC), respectively. Nevertheless, these studies have been restricted to gangliosides containing the N-acetylated variant of sialic acid (NeuAc) [24, 25]. …”

Section: Discussionmentioning

confidence: 99%

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Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Primary Lymphoid Tumors and Lymph Node Metastasis

Blanco

Quintana

et al. 2013

Pathology Research International

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The reactivity of the 14F7 Mab, a highly specific IgG1 against N-glycolyl GM3 ganglioside (NeuGcGM3) in normal tissues, lymphomas, lymph node metastasis, and other metastatic sites was assessed by immunohistochemistry. In addition, the effect of chemical fixation on the 14F7 Mab staining using monolayers of P3X63Ag.653 cells was also evaluated. Moreover, the ability of 14F7 to bind NeuGcGM3 ganglioside inducing complement-independent cytotoxicity by a flow cytometry-based assay was measured. The 14F7 Mab was reactive in unfixed, 4% paraformaldehyde, 4% formaldehyde, and acetone fixed cells. Postfixation with acetone did not alter the localization of NeuGcGM3, while the staining with 14F7 Mab was significantly eliminated in both cells fixed and postfixed with methanol but only partially reduced with ethanol. The staining with 14F7 Mab was evidenced in the 89.2%, 89.4%, and 88.9% of lymphomas, lymph node metastasis, and other metastatic sites, respectively, but not in normal tissues. The treatment with 14F7 Mab affected both morphology and membrane integrity of P3X63Ag.653 cells. This cytotoxic activity was dose-dependent and ranged from 24.0 to 84.7% (10–1000 μg/mL) as compared to the negative control. Our data could support the possible use of NeuGcGM3 as target for both active and passive immunotherapy against malignancies expressing this molecule.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Primary Lymphoid Tumors and Lymph Node Metastasis

Blanco

Quintana

et al. 2013

Pathology Research International

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“…Ganglioside expression on malignant cells differs from normal tissue. GM2 is more highly expressed on cell surface of cancers of neuroectodermal origin and lymphoma cells 6 7. In fact, GM2 and its components were the temporary targets for vaccination in human melanoma patients and lymphoma in mouse model 8 9.…”

Section: Discussionmentioning

confidence: 99%

Acute inflammatory neuropathy with monoclonal anti-GM2 IgM antibodies, IgM-κ paraprotein and additional autoimmune processes in association with a diffuse large B-cell non-Hodgkin's lymphoma

et al. 2013

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Lymphoproliferative disorders are often associated with autoimmune processes preceding or following the occurrence of a lymphoma. Here, we describe a patient with a history of recurrent diffuse large B-cell non-Hodgkin's lymphoma who suffered from an acute inflammatory neuropathy with specific monoclonal anti-GM2 IgM antibodies and associated IgM-κ paraprotein. It was possible in this case to prove that both, anti-GM2 IgM antibodies and IgM-κ paraprotein, share the same binding characteristic. In addition, the patient possibly suffered from an immune thrombocytopenia and an early-stage bullous pemphigoid with anti-BP-230 IgG antibodies. Intravenous immunoglobulin and plasmapheresis alleviated the acute neuropathy and thrombocytopenia, while the bullous pemphigoid has been aggravated. In summary, the simultaneous occurrence of multiple autoimmune processes was a sign of a dysfunctional immune system preceding the relapse of a B-cell non-Hodgkin's lymphoma.

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“…Gangliosides, GM2 [GalNAcβ1-4 (NeuAcα2-3)Galβ1-4Glcβ1-1Cer] and GD2 [GalNAcβ1-4(NeuAcα2-8NeuAcα2-3)Galβ1-4Glcβ1-1Cer] have been well characterized as cell surface tumor-associated antigens on neuro-ectodermal origin tumors such as melanoma cells [13, 14, 15, 16, 17, 18]. Overexpression of GM2 and/or GD2 have been reported in other types of cancers such as renal cell carcinoma, neuroblastoma, gastric cancer, adult leukemia, lung carcinoma, and glioma [19, 20, 21, 22, 23, 24, 25]. GalNAcT (β1,4N-acetylgalactosaminyltransferase) is a glycosyltransferase involved in the synthesis of GM2 and GD2 from precursors, GM3 and GD3, respectively [26].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of β1,4N-Acetylgalactosaminyl- transferase mRNA as a Molecular Marker for Various Types of Cancers

Sugita

Fujiwara²,

Hoon³

et al. 2002

Oncology

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Objective: To determine GalNAcT mRNA expression in human carcinoma cell lines and primary tumor tissues. Assessment of the potential use of GalNAcT mRNA as a molecular marker for detection of metastatic cancer cells in the peripheral blood of patients with hepatocellular carcinoma. Methods/Results: We investigated GalNAcT mRNA expression in various human cancer cell lines and primary cancer tissues using RT-PCR assay for GalNAcT mRNA. The expression of GalNAcT mRNA was detected in 25 of 26 cancer cell lines tested and in the majority of primary tumors from different organs: 8 of 10 colon cancers, 9 of 9 breast cancers, 11 of 12 esophageal cancers, 14 of 14 gastric cancers, 4 of 18 pancreatic cancers, 6 of 12 biliary tract cancers, 17 of 18 hepatocellular carcinomas and 13 of 14 lung cancers. Semi-quantitative analysis with duplex RT-PCR showed that the amount of the GalNAcT mRNA was enhanced in cancer tissues as compared to the surrounding cancer-free tissues. Blood specimens of 5 of 14 patients with hepatocellular carcinoma were positive for GalNAcT mRNA, all of whom developed recurrent disease in less than 24 months. Peripheral blood samples of 30 normal subjects were negative for GalNAcT mRNA. Conclusion: Our results suggest that the RT-PCR assay for GalNAcT mRNA could be a potentially useful molecular marker for detecting cancer dissemination in blood circulation of patients with malignancy.

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Gangliosides in acute myeloid leukaemia (AML) and non-hodgkin's lymphoma (NHL)

Cited by 13 publications

References 10 publications

Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Primary Lymphoid Tumors and Lymph Node Metastasis

Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Primary Lymphoid Tumors and Lymph Node Metastasis

Acute inflammatory neuropathy with monoclonal anti-GM2 IgM antibodies, IgM-κ paraprotein and additional autoimmune processes in association with a diffuse large B-cell non-Hodgkin's lymphoma

Overexpression of β1,4N-Acetylgalactosaminyl- transferase mRNA as a Molecular Marker for Various Types of Cancers

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