Gangliosides attenuate vincristine neurotoxicity on dorsal root ganglion cells

Houi, Kouji; Mochio, Soichiro; Kobayashi, Takaaki

doi:10.1002/mus.880160104

Cited by 7 publications

(2 citation statements)

References 12 publications

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“…This has previously been demonstrated in a rat glioma cell line. 24 Several attempts have been made to alter microtubule assembly, which would then function as an antineoplastic therapy, combining vinca alkaloids with additional agents, 50,51,54 or relying on other agents with similar action. 6,19,25,45 This mechanism, at least in part, accounts for one of the side effects of vinca alkaloids, reversible polyneuropathy.…”

Section: Vinca Alkaloids Dph and Microtubulesmentioning

confidence: 99%

Differential effects of vincristine and phenytoin on the proliferation, migration, and invasion of human glioma cell lines

Tonn¹,

Haugland²,

Saraste³

et al. 1995

Journal of Neurosurgery

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The aim of this study was to investigate the antimigratory and antiinvasive potential of vincristine sulfate (VCR) on human glioma cells and to analyze whether phenytoin (5,5-diphenylhydantoin; DPH) might act synergistically with VCR. Vincristine affects the cytoplasmic microtubules; DPH has been reported to enhance VCR cytotoxicity in murine cells. In two human glioma cell lines, GaMG and D-37MG, we found VCR to reduce monolayer growth and colony formation in a dose-dependent fashion at concentrations of 10 ng/ml and above. Phenytoin increased the cytotoxic and cytostatic effects of VCR in monolayer cells but not in spheroids. Multicellular spheroids were used to investigate directional migration. A coculture system of GaMG and D-37MG spheroids with fetal rat brain aggregates was used to analyze and quantify tumor cell invasion. A dose-dependent inhibition of migration and invasion by VCR was observed in both cell lines without further enhancement by DPH. Immunofluorescence microscopy with antibodies against alpha-tubulin revealed dose-dependent morphological alterations in the microtubules when the cells were exposed to VCR but not after incubation with DPH. Based on the combination of standardized in vitro model systems currently in use and the present data, the authors strongly suggest that VCR inhibits migration and invasion of human glioma cells. This is not altered by DPH, which inhibits cell proliferation in combination with VCR.

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Section: Vinca Alkaloids Dph and Microtubulesmentioning

confidence: 99%

Differential effects of vincristine and phenytoin on the proliferation, migration, and invasion of human glioma cell lines

Tonn¹,

Haugland²,

Saraste³

et al. 1995

Journal of Neurosurgery

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show abstract

“…Gangliosides attenuated vincristine neurotoxicity in vitro (Houi et al, 1993) and substantially reduced vincristine-induced electrophysiological alterations in rabbit sciatic nerve (Favaro et al, 1988). Gangliosides, 40 mg intramuscularly twice weekly, prevented vincristine (1 mg/week) neurotoxicity in 2 of 7 patients (Oka et al, 1989), whereas Cronassial W , a mixture of four naturally occurring gangliosides, 100 mg/day intramuscularly, provided no neuroprotection (DeAngelis et al, 1991).…”

Section: Vincristinementioning

confidence: 99%