Ganciclovir-loaded albumin nanoparticles: characterization and in vitro release properties

Merodio, Marta; Arnedo, A; Renedo, M. J.; Irache, Juan M.

doi:10.1016/s0928-0987(00)00169-x

Cited by 199 publications

(126 citation statements)

References 17 publications

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“…Levels of glutaraldehyde and pH were assigned as per some early studies (6,23). Incubation time levels were selected according to Merodio et al (7).…”

Section: Optimization Of the Preparation Conditions For 5-fluorouracimentioning

confidence: 99%

“…Colloidal systems based on proteins might be incredibly promising as they unite the advantages of nanoparticles with the advantages of covalent protein-drug conjugates (5,6). Protein nanoparticles, due to the existence of charged groups, may also be used as a matrix in which drugs can be physically entrapped (7)(8)(9). Albumin, being widespread in nature, is an attractive macromolecular carrier and has been a subject of major interest.…”

Section: Introductionmentioning

confidence: 99%

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5-Fluorouracil-Loaded BSA Nanoparticles: Formulation Optimization and In Vitro Release Study

2008

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Abstract. Over the past few decades, there has been considerable interest in developing protein nanoparticles as drug delivery devices. The underlying rationale is their exceptional characteristics, namely biodegradability and nonantigenicity. Herein, phase separation method was used to prepare 5-fluorouracil-loaded bovine serum albumin (BSA) nanoparticles. Drug release was tracked by continuous flow dialysis technique. Effect of process variables on loading efficiency of 5-fluorouracil was investigated and optimized through Taguchi's M16 design with the amount of entrapped drug as response. Optimum condition was found to be 2 mg/mL of 5-fluorouracil, 3.7 mL of added ethanol, 176 µL of glutaraldehyde, drug-protein incubation time of 30 min, and pH of 8.4 for 200 mg of BSA in 2 mL drug solution. pH had the most noticeable effect on the amount of entrapped drug, but glutaraldehyde had the least. Mean diameter and zeta potential of fabricated nanoparticles under these conditions were 210 nm and −31.7 mV, respectively. Drug-loaded BSA nanoparticles suspension maintained constant release of drug for 20 h under experimental conditions, so this colloidal drug carrier is capable of releasing drug in a sustained manner.

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“…Levels of glutaraldehyde and pH were assigned as per some early studies (6,23). Incubation time levels were selected according to Merodio et al (7).…”

Section: Optimization Of the Preparation Conditions For 5-fluorouracimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

5-Fluorouracil-Loaded BSA Nanoparticles: Formulation Optimization and In Vitro Release Study

2008

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“…Among them, nanoparticulate systems based on proteins have gained much interest because of their inherent properties of biodegradability, lack of toxicity, and nonantigenicity (1,2). Protein nanoparticles may be used as a matrix in which a wide variety of drugs can be physically entrapped (3)(4)(5). In contrast to other nanoparticulate systems, they offer the benefit of carrying functional groups such as amino and carboxylic groups that can be used for surface modification (6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Optimization of PEGylation Conditions for BSA Nanoparticles Using Response Surface Methodology

et al. 2010

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Abstract. Chemical coupling of polyethylene glycol (PEG) to proteins or particles (PEGylation), prolongs their circulation half-life by greater than 50-fold, reduces their immunogenicity, and also promotes their accumulation in tumors due to enhanced permeability and retention effect. Herein, phase separation method was used to prepare bovine serum albumin (BSA) nanoparticles. PEGylation of BSA nanoparticles was performed by SPA activated mPEG through their free amino groups. Effect of process variables on PEGylation efficiency of BSA nanoparticles was investigated and optimized through response surface methodology with the amount of free amino groups as response. Optimum conditions was found to be 32.5 g/l of PEG concentration, PEG-nanoparticle incubation time of 10 min, incubation temperature of 27°C, and pH of 7 for 5 mg of BSA nanoparticles in 1 mL phosphate buffer. Analysis of data showed that PEG concentration had the most noticeable effect on the amount of PEGylated amino groups, but pH had the least. Mean diameter and zeta potential of PEGylated nanoparticles under these conditions were 217 nm and −14 mV, respectively. In conclusion, PEGylated nanoparticles demonstrated reduction of the negative surface charge compared to the non modified particles with the zeta potential of −31.7 mV. Drug release from PEGylated nanoparticles was almost slower than non-PEGylated ones, probably due to existence of a PEG layer around PEGylated particles which makes an extra resistance in opposition to drug diffusion.

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“…18 Briefly, albumin (2% w/v) was dissolved in deionized water and the pH was adjusted to 7. Resveratrol 10 mg was dissolved in 200 µL of ethanol and added dropwise to the albumin solution and incubated for one hour in the dark at room temperature.…”

Section: Preparation Of Rnpsmentioning

confidence: 99%

Polycaprolactone scaffold engineered for sustained release of resveratrol: therapeutic enhancement in bone tissue engineering

Kamath¹,

Ahmed²,

Dhanasekaran³

et al. 2013

IJN

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Biomaterials-based three-dimensional scaffolds are being extensively investigated in bone tissue engineering. A potential scaffold should be osteoconductive, osteoinductive, and osteogenic for enhanced bone formation. In this study, a three-dimensional porous polycapro-lactone (PCL) scaffold was engineered for prolonged release of resveratrol. Resveratrol-loaded albumin nanoparticles (RNP) were synthesized and entrapped into a PCL scaffold to form PCL-RNP by a solvent casting and leaching method. An X-ray diffraction study of RNP and PCL-RNP showed that resveratrol underwent amorphization, which is highly desired in drug delivery. Furthermore, Fourier transform infrared spectroscopy indicates that resveratrol was not chemically modified during the entrapment process. Release of resveratrol from PCL-RNP was sustained, with a cumulative release of 64% at the end of day 12. The scaffold was evaluated for its bone-forming potential in vitro using human bone marrow-derived mesenchymal stem cells for 16 days. Alkaline phosphatase activity assayed on days 8 and 12 showed a significant increase in activity (1.6-fold and 1.4-fold, respectively) induced by PCL-RNP compared with the PCL scaffold (the positive control). Moreover, von Kossa staining for calcium deposits on day 16 showed increased mineralization in PCL-RNP. These results suggest PCL-RNP significantly improves mineralization due to its controlled and prolonged release of resveratrol, thereby increasing the therapeutic potential in bone tissue engineering.

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Ganciclovir-loaded albumin nanoparticles: characterization and in vitro release properties

Cited by 199 publications

References 17 publications

5-Fluorouracil-Loaded BSA Nanoparticles: Formulation Optimization and In Vitro Release Study

5-Fluorouracil-Loaded BSA Nanoparticles: Formulation Optimization and In Vitro Release Study

Optimization of PEGylation Conditions for BSA Nanoparticles Using Response Surface Methodology

Polycaprolactone scaffold engineered for sustained release of resveratrol: therapeutic enhancement in bone tissue engineering

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