gaMS-1 : a gametophytic male sterile mutant in maize

Sari‐Gorla, M.; Ferrario, S.; Villa, Matteo; Pè, Mario Enrico

doi:10.1007/s004970050034

Cited by 11 publications

(18 citation statements)

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“…According to Sari-Gorla et al (1997), mutations that act gametophytically affecting the nuclear division pattern are scarcely represented. Mutants of gametophytic action have been reported in Arabidopsis (McCormick et al 1994;Xu et al 1995;Chen and McCormick 1996;Park et al 1998) and in maize (Zea mays) (Sari-Gorla et al 1996, 1997. None was similar to that found in the soybean line under analysis.…”

Section: Resultsmentioning

confidence: 68%

An original mutation in soybean (Glycine max(L.) Merrill) involving degeneration of the generative cell and causing male sterility

Bione

Pagliarini²,

Almeida³

2002

Genome

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A spontaneous mutation causing male sterility has been detected in line BR97-17739 from the soybean breeding program conducted by Embrapa-National Soybean Research Center. Meiotic division and male gametophyte development were analyzed in 10 male-sterile, female-fertile plants. Meiotic process had few irregularities related to chromosome segregation and affected about 2% of tetrads. Despite the high frequency of normal microspores, pollen sterility was total. After callose dissolution, microspores were released into the anther loculle and interphase nucleus was displaced from the center to one side of the cell. Displacement continued throughout normal microspore mitosis (PMI). After telophase, the hemispherical phragmoplast marked the place of cytokinesis. A typical generative cell, adjacent to the plasma membrane, and the vegetative one, containing most of the cytoplasm, were formed. In spite of the well-formed generative cell, pollen mitosis (PMII) failed to occur. The generative cell degenerated and was completely destroyed. The 3:1 segregation for male sterility in this line and its progenies indicate that a single recessive gene controls mutation.

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Section: Resultsmentioning

confidence: 68%

An original mutation in soybean (Glycine max(L.) Merrill) involving degeneration of the generative cell and causing male sterility

Bione

Pagliarini²,

Almeida³

2002

Genome

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“…However, ms32, which is located in the 2.08 bin, is upstream from the SSR mapping front marker bnlg1940. Additionally, among the reported male sterile genes located on chromosome 2, only gaMs1 shows pollen abortion at the microspore stage (Supplemental Table 2; Sari-Gorla et al 1996;Fink et al 2001). However, gaMs1, which belongs to gametophytic male sterility rather than sporophytic male sterility, is located in the short arm of chromosome 2, which is far from the location of K305ms (Fink et al 2001).…”

Section: Discussionmentioning

confidence: 97%

Characterization and genetic mapping of a novel recessive genic male sterile gene ms305 in maize (Zea mays L.)

Wang¹,

Gu²,

Chen³

et al. 2015

Israel Journal of Plant Sciences

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The male sterility system shows tremendous value for the long-term utilization of hybrid crop breeding. In this study, a male sterile mutant, K305ms, was derived from the M3 progeny of maize (Zea mays L.) inbred line K305 exposed to 60Co-γ irradiation. Male sterile K305ms plants did not show any obvious differences from their sibling male fertile plants (K305F) during the vegetative stage, but failed to produce functional pollen at the reproductive stage. Microscopic observations determined that the dyads and tetrads from the pollen of K305ms plants developed abnormally, and subsequently the microspores were shriveled. Genetic analysis indicated that the male sterility of K305ms was controlled by a single recessive genic gene. Gene mapping showed that the responsible gene was located between two simple sequence repeat markers on chromosome 2L in a region of 10.3 cM, bnlg469b and bnlg1940, with genetic distances of 2.9 and 7.4 cM, respectively. According to the microscopic and mapping characteristics, our results showed that this gene was distinguishable from all other reported male sterile genes in maize, and it is temporarily designated as ms305. The linkage map in this study will provide a useful fundamental basis for molecular marker-assisted selection as well as for further map-based cloning of ms305.

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“…So far, only a small number of genes essential for pollen development have been isolated and characterised from various plants (Hamilton and Mascarenhas 1997 (review); Liu et al 2001;Kapoor et al 2002;Zhao et al 2002). In order to isolate and characterise genes involved in maize pollen development, Sari-Gorla et al (1996 produced two gametophytically expressed male-sterile mutants of maize by transposon insertion mutagenesis. One of these mutants, gaMS-2, is expressed immediately after the first pollen mitosis and produces unregulated nuclear divisions, eventually leading to complete chromatin fragmentation.…”

Section: Introductionmentioning

confidence: 99%

Male-sterile mutation alters Zea�m�1 (�-expansin�1) accumulation in a maize mutant

Wang

Scali

Vignani

et al. 2004

Sexual Plant Reproduction

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gaMS-2 is a gametophytic male-sterile mutant of maize, with sterile pollen grains developmentally blocked at the binucleate stage. To characterise differentially expressed proteins in gaMS-2 pollen, we compared protein profiles of anthers and mature pollen from heterozygous GaMS-2/gaMS-2 plants and wild type (wt) plants by two-dimensional electrophoresis (2-DE). A basic protein present at a greatly reduced level in GaMS-2/gaMS-2 anthers was subsequently identified by tandem mass spectrometry as Zea m 1 (a glycoprotein of 31 kDa), the major group-1 allergen of maize pollen and a member of the b-expansin 1 family. Moreover, we show that Zea m 1 can be deglycosylated by peptide Nglycosidase F. After deglycosylation, four major isoforms-Zea m 1a (more acetic), Zea m 1b, Zea m1c and Zea m 1d (more basic)-can be discriminated in wt anther in 2-DE immunoblots probed with a monoclonal antibody against the group-1 pollen allergen, whereas all the isoforms, especially Zea m 1a, exist at reduced levels in GaMS-2/gaMS-2 anthers. Furthermore, the reduced Zea m 1 accumulation in the mutant appears to occur in immature pollen but not in anther sporophytic tissues. Finally, we separated sterile pollen grains (at the mononucleate stage) from fertile ones using 42% Percoll solution, and found that Zea m 1 is barely detectable in sterile pollen grains. Together, our results indicate that a reduced Zea m 1 level is associated with the sterile phenotype of gaMS-2.

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gaMS-1 : a gametophytic male sterile mutant in maize

Cited by 11 publications

References 0 publications

An original mutation in soybean (Glycine max(L.) Merrill) involving degeneration of the generative cell and causing male sterility

An original mutation in soybean (Glycine max(L.) Merrill) involving degeneration of the generative cell and causing male sterility

Characterization and genetic mapping of a novel recessive genic male sterile gene ms305 in maize (Zea mays L.)

Male-sterile mutation alters Zea�m�1 (�-expansin�1) accumulation in a maize mutant

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