Gamma radiation effect on vascular smooth muscle cells in culture

Cobalt-60 gamma radiation increased the nitric oxide generation in cultured rat vascular smooth muscle cells

“…Previous report showed that radiation inhibited the proliferation of cultured VSMCs by damaging their chromosomes in a dose dependent manner (Gajdusek et al, 1996). In this study, radiation at the dose of 14 and 25Gy significantly promoted the expression of iNOS gene in VSMCs, indicating that the increased iNOS activity is, at least partially, through the up-regulation of its gene.…”

Progression of Atheroma

“…Vascular SMCs and ECs encounter a broad variety of biologically active environmental factors, such as mechanical force, [31][32][33][34][35] oxidative stress, 36 radiation, [37][38][39][40][41] reactive oxygen or nitrogen species, [42][43][44][45] lipids (cholesterol and its oxides), 16,41,46 -48 viral 49 -51 and bacterial products, 52 and inflammatory cytokines. 7,53,54 Many of these extracellular environmental and immunologic factors can induce apoptosis in vascular cells (Table 2).…”

Ionizing Radiation to Prevent Arterial Intimal Hyperplasia At the Edges of the Stent: Induces Necrosis and Fibrosis

“…Second, a previous study conducted in our laboratory using the same experimental model showed that doses exceeding 15 Gy are necessary to prevent intimal hyperplasia [15]. In addition, the doses we used matched those recommended for animal experiments and also for clinical use (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) [19].…”

“…60 recommended a dose ranging from 15 to 30 Gy [19]. In a previous clinical study, Waksman et al showed that high doses (Ͼ14 -28 Gy) significantly decrease cell proliferation and intimal hyperplasia at 3 and 7 days after arterial aggression, but as others have noted, apoptosis remains unchanged [23,24]. Recently, in our laboratory, we provided evidence that a single high dose (20 Gy) of ionizing radiation significantly decreases the number of proliferative cells within the arterial wall in a few days after aggression but that in a 6-week period the total number of immediate and delayed proliferative cells remains unchanged [25].…”