Gamma irradiation of aloe-emodin induced structural modification and apoptosis through a ROS- and caspase-dependent mitochondrial pathway in stomach tumor cells

Abstract

The radiolysis product (AEF1) of aloe-emodin transformed by gamma-irradiation strongly induced apoptotic cell death in AGS cells, indicating AEF1 is a potential candidate drug for use in anti-cancer drug.

Investigation on the Characteristic Components of Dahuang Zhechong Pill Based on High-Performance Liquid Chromatography (HPLC) Fingerprint

“…15 Similarly, aloe emodin showed a strong anti-tumor effect by inducing tumor cell apoptosis. 16,17 In our study, similar experimental results were obtained, as the anthraquinone components in DHZCP showed a strong inhibitory effect in SMMC-7721/DOX cells.…”

Radiolytic Cyclization Products of Phloridzin as Potent Anti‐Glycation Agents

“…Meth-anolic radiolysis is recognized to mediate specific chemical changes associated with sufficient production of reactive oxygen species in the natural products of chalcone, anthraquinone, and flavonoids. [32][33][34][35] Our results support that the hydroxymethyl radical may also be implicated in the generation of 2 and 3 during γ-irradiation of phloridzin in MeOH. This is the first example of a new radiolytic cyclization of dihydrochalcone phloridzin.…”

AMPK-mediated phosphorylation on 53BP1 promotes c-NHEJ

“…Phosphorylation of Thr172 was essential for AMPK activation, which is directly phosphorylated by two upstream kinase, LKB1 and CaMKKb. Irradiation robustly activated AMPK in first 30 min, which was partially attributed to a dramatic induction of reactive oxygen species (ROS) stimulated by X-ray (Byun et al, 2018), as treatment with the ROS scavenger Trolox atten-uated AMPK activation (Figure 3A; Figures S3A and S3B). ATM has been reported to phosphorylate LKB1 during DSB stress, which is a well-established upstream kinase of AMPK (Ui et al, 2014).…”