Gamma irradiation induced effects on in vitro shoot cultures and influence of monochromatic light regimes on irradiated shoot cultures of Dendrobium sonia orchid

Billore, Vandita; Mirajkar, S. J.; Suprasanna, Penna; Jain, Monica

doi:10.1016/j.btre.2019.e00343

Cited by 21 publications

(9 citation statements)

References 38 publications

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“…These results agree with the previous report of Taheri et al (2014) indicating significant differences in leaf characters and plant height among gamma-induced mutants of Curcuma alismatofolia and those of 15-Gy irradiated Graptophyllum pictum (Rosmala et al, 2016). Generally, Billore et al (2019) claimed that reduced plant stature or dwarfism is a common expression of induced mutation among organisms. Similarly, Abdullah et al (2018) also sighted dwarfing effect on mutants of Zingiber officinale Roscoe mutants irradiated with 9-13 Gy, while Billore et al (2019) reported reduced shoot length and leaf area in Dendrobium 'Sonia Kai' irradiated with 15 to 45 Gy.…”

Section: Morphological Characterization Of 15-gy Irradiated P Aphrodi...supporting

confidence: 92%

“…The pods of the 15-Gy irradiated plant selections were shorter compared with the unirradiated P. aphrodite plants. The aforementioned findings suggest that there is dwarfing effect brought about by irradiation which is a common phenomenon in mutation breeding (Billore et al, 2019). This was evident not only in the leaf characteristics of P. aphrodite but also in the other plant traits, such as spike length and distance between flowers on the spike.…”

Section: Morphological Characterization Of 15-gy Irradiated P Aphrodi...mentioning

confidence: 87%

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Evaluation of Plant and Flower Characteristics of selected 15-Gy irradiated Phalaenopsis aphrodite

Magdalita,

San Pascual,

Villareal

2022

MJST

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Phalaenopsis or butterfly orchid is found in tropical countries and can be classified as terrestrial, epiphytic, or lithophytic. Because Phalaenopsis aphrodite’s flowers are purely white, attempts were made to modify them through gamma irradiation. A 15-Gy dose of gamma irradiation effectively induced mutation in P. aphrodite. In this study, three mutant plant selections, previously irradiated with 15 Gy, were characterized based on the leaf, root and flower traits and compared these traits with that of the untreated P. aphrodite. The plant selections have purple to light purple flowers in contrast to the control plants with pure white flowers. Flower, leaf and root characters of irradiated plants were observed to be significantly different from untreated plants. Irradiated plants are smaller, compact and dwarf at the flowering stage. In addition, more primary roots and increased chlorophyll content were observed in irradiated plants. They also have shorter spikes with lesser flowers. Furthermore, smaller flowers and thinner leaves were also detected in the irradiated plants. In addition, after self-pollination, irradiated plants obtained shorter pods. Currently, the three 15-Gy irradiated P. aphrodite plants are being self-pollinated and grown in vitro. They will then be further grown to assess the stability of phenotypic traits observed in the first generation.

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Section: Morphological Characterization Of 15-gy Irradiated P Aphrodi...supporting

confidence: 92%

Section: Morphological Characterization Of 15-gy Irradiated P Aphrodi...mentioning

confidence: 87%

Evaluation of Plant and Flower Characteristics of selected 15-Gy irradiated Phalaenopsis aphrodite

Magdalita,

San Pascual,

Villareal

2022

MJST

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“…In breeding programs using in vitro techniques, PLBs could be used to obtain autotetraploid plants with use of anti-mytotic agents as oryzalin [142] and colchicine [143], and to obtain mutants by the use of chemical mutagens as sodium azide [144] or physical mutagens as gamma-irradiation [145].…”

Section: Applications Of Ipr-plb Technique On Orchid Propagation and mentioning

confidence: 99%

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

Cardoso

Zanello

Chen

2020

IJMS

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The process through induction, proliferation and regeneration of protocorm-like bodies (PLBs) is one of the most advantageous methods for mass propagation of orchids which applied to the world floricultural market. In addition, this method has been used as a tool to identify genes of interest associated with the production of PLBs, and also in breeding techniques that use biotechnology to produce new cultivars, such as to obtain transgenic plants. Most of the molecular studies developed have used model plants as species of Phalaenopsis, and interestingly, despite similarities to somatic embryogenesis, some molecular differences do not yet allow to characterize that PLB induction is in fact a type of somatic embryogenesis. Despite the importance of species for conservation and collection purposes, the flower market is supported by hybrid cultivars, usually polyploid, which makes more detailed molecular evaluations difficult. Studies on the effect of plant growth regulators on induction, proliferation, and regeneration of PLBs are the most numerous. However, studies of other factors and new technologies affecting PLB production such as the use of temporary immersion bioreactors and the use of lighting-emitting diodes have emerged as new tools for advancing the technique with increasing PLB production efficiency. In addition, recent studies on Phalaenopsis equestris genome sequencing have enabled more detailed molecular studies and the molecular characterization of plantlets obtained from this technique currently allow the technique to be evaluated in a more comprehensive way regarding its real applications and main limitations aiming at mass propagation, such as somaclonal variation.

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“…Hasil iradiasi gamma pada generasi ketiga planlet Celosia cristata menunjukkan terjadi perubahan morfologi pada planlet antara lain terlihat pada memendeknya ruas dan mengeritingnya daun, terutama pada dosis LD 50 , LD 30 dan LD 20 dari C. cristata yaitu 68.73 Gy, 46.68 Gy, dan 35.65 Gy (Hayati et al, 2016). Perlakuan induksi mutasi dengan sinar gamma diharapkan dapat memunculkan karakter baru pada anggrek (Lestari et al, 2018) antara lain warna bunga, ukuran, morfologi, umur simpan, arsitektur dan kekuatan tanaman (vigor) (Billore et al, 2019).…”

Section: Pendahuluanunclassified

Radiosensitivitas dan Pertumbuhan In Vitro Protocorm Anggrek ‘Tien Soeharto’ (Cymbidium hartinahianum J.B. Comber & R.E. Nasution)

Handini

Sukma²,

Sudarsono

et al. 2020

J. Agron. Indonesia

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C. hartinahianum J.B. Comber & R.E. Nasution merupakan salah satu jenis anggrek endemik di Sumatera Utara. Habitatnya terbatas di dataran tinggi dengan suhu rendah. Habitat alami tersebut sudah banyak dikonversi untuk penggunaan lainnya. Induksi radiasi sinar gamma merupakan metode alternatif untuk mendapatkan tanaman mutan toleransi terhadap kondisi suhu yang tinggi. Penelitian ini bertujuan untuk mengetahui radiosensitivitas protocorm C. hartinahianum J.B. Comber & R.E. Nasution terhadap iradiasi sinar gamma dan pertumbuhan PLBs di suhu ruang kultur yang berbeda. Protocorm diiradiasi dalam kisaran dosis iradiasi 0, 5, 10, 15, dan 20 Gy, kemudian separoh populasi protocorm pasca iradiasi diinkubasi dalam ruang kultur dengan suhu 16-18 oC dan separoh yang lain di suhu 22-27 oC, untuk mengevaluasi radiosentitivitas dan pertumbuhannya. Hasil penelitian menunjukkan bahwa LD20 dan LD50 protocorm di ruang kultur bersuhu 16-18 oC adalah 26.98 Gy dan 38.24, sementara LD20 dan LD50 protocorm pada ruang kultur bersuhu 22-27 oC adalah 17.08 Gy dan 27.29 Gy. Proliferasi PLBs dan morfogenesis tunas C. hartinahianum lebih baik pada ruang kultur dengan suhu 22-27 oC. Hasil penelitian menunjukkan bahwa re-iradiasi sinar gamma sebaiknya dilakukan pada LD20 atau LD50 dari ruang kultur dengan suhu 22-27 oC yaitu sebesar 17.08 Gy dan 27.29 Gy, untuk mendapatkan mutan putatif C. hartinahianum. Kata kunci: dosis letal, induksi mutasi in vitro, iradiasi sinar gamma, multiplikasi, protocorm like bodies (PLBs)

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Gamma irradiation induced effects on in vitro shoot cultures and influence of monochromatic light regimes on irradiated shoot cultures of Dendrobium sonia orchid

Cited by 21 publications

References 38 publications

Evaluation of Plant and Flower Characteristics of selected 15-Gy irradiated Phalaenopsis aphrodite

Evaluation of Plant and Flower Characteristics of selected 15-Gy irradiated Phalaenopsis aphrodite

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

Radiosensitivitas dan Pertumbuhan In Vitro Protocorm Anggrek ‘Tien Soeharto’ (Cymbidium hartinahianum J.B. Comber & R.E. Nasution)

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