Gamma Irradiation Increases hsp-70 in Chinese Hamster Ovary Cells

Sierra-Rivera, Elaine; Voorhees, Gerard J.; Freeman, Michael L.

doi:10.2307/3578394

Cited by 59 publications

(27 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Verbenko and Kalinin (1995) showed that several Hsps were induced by gamma rays in E. coli K-12 in which the SOS responses are constitutively expressed, and an interaction of the two stress systems in bacterial cells is possible. In addition, DnaK has been reported to be induced by gamma rays in eukaryotic cells (Sierra-Rivera et al 1993) to induce radioresistance (Park et al 2000) and to prevent radiation-induced cell death (Lee et al 2001). According to Calini et al (2003), DnaK overexpression acts as a radioprotective mechanism towards the first event of DNA damage and increases long-term viability.…”

Section: Discussionmentioning

confidence: 99%

Effect of gamma radiation on heat shock protein expression of four foodborne pathogens

Caillet

Millette

Dussault

et al. 2008

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: The effects of gamma radiation on three heat shock proteins (Hsps) (GroEL, DnaK and GroES) synthesis in two Gram‐negative (Escherichia coli and Salmonella serotype Typhimurium) and two Gram‐positive (Staphylococcus aureus and Listeria monocytogenes) bacteria were investigated. Methods and Results: The bacterial strains were treated with three radiation doses to induce cell damage, to obtain a viable but nonculturable state, and to cause cell death. Western blot analysis and quantification of Hsps in bacteria were performed immediately after irradiation treatment. In the four foodborne pathogens, GroEL was strongly induced by gamma rays in a dose‐dependent manner, confirming the involvement of this protein in the cellular response to the stress generated by ionizing radiation. In addition, it was found that E. coli exposed to gamma radiation showed a significantly induction of DnaK and GroES proteins when compared with nonirradiated bacteria, whereas a GroES slight induction and a DnaK inhibition were observed in Salm. Typhimurium. Conclusions: The gamma rays influence the synthesis of Hsps in foodborne pathogen in a way that critically depends on the radiation dose. Significance and Impact of the Study: The study of stress response to several radiation doses was undertaken to elucidate how bacteria can survive in harsh conditions and cope with gamma radiation used to control foodborne pathogens and to characterize their adaptative response to this treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of gamma radiation on heat shock protein expression of four foodborne pathogens

Caillet

Millette

Dussault

et al. 2008

Journal of Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…20 Also, radiation has been found to affect cytosolic Hsp70 levels in tumor cells. [21][22][23] Since g-irradiation is frequently used in cancer therapy, 24,25 it is important to understand the molecular nature of radiation-induced effects on resistance of tumor cells to apoptosis. 26,27 Therefore, we investigated radiation-induced effects in three independent carcinoma cell systems that differ with respect to their capacity to express Hsp70 on their cell surface.…”

Section: Introductionmentioning

confidence: 99%

Dual function of membrane-bound heat shock protein 70 (Hsp70), Bag-4, and Hsp40: protection against radiation-induced effects and target structure for natural killer cells

et al. 2004

View full text Add to dashboard Cite

CX þ /CXÀ and Colo þ /ColoÀ tumor sublines with stable heat shock protein 70 (Hsp70) high and low membrane expression were generated by fluorescence activated cell sorting of the parental human colon (CX2) and pancreas (Colo357) carcinoma cell lines, using an Hsp70-specific antibody. Two-parameter flow cytometry revealed that Hsp70 colocalizes with Bag-4, also termed silencer of death domain, not only in the cytosol but also on the plasma membrane. After nonlethal c-irradiation, the percentage of membranepositive cells and the protein density of Hsp70 and Bag-4 were found to be strongly upregulated in carcinoma sublines with initially low expression levels (CXÀ, ColoÀ). Membrane expression of Hsp70 was also elevated in Bag-4 overexpressing HeLa cervix carcinoma cells when compared to neo-transfected cells. In response to c-irradiation, neotransfected HeLa cells behaved like Hsp70/Bag-4 lowexpressing CXÀ and ColoÀ, and Bag-4-transfected HeLa cells like Hsp70/Bag-4 high-expressing carcinoma sublines CX þ and Colo þ . Immunoprecipitation studies further confirmed colocalization of Hsp70 and Bag-4 but also point to an association of Hsp70 and Hsp40 on the plasma membrane of CX þ and Colo þ cells; on CXÀ and ColoÀ tumor sublines, Hsp40 was detectable in the absence of Hsp70 and Bag-4. Other co-chaperones including Hsp60 and Hsp90 were neither found on the cell surface of CX þ /CXÀ, Colo þ /ColoÀ nor on HeLa neo-/HeLa Bag-4-transfected tumor cells. Functionally, Hsp70/Bag-4 and Hsp70/Hsp40 membrane-positive tumor cells appeared to be better protected against radiation-induced effects, including G2/M arrest and growth inhibition, on the one hand. On the other hand, membrane-bound Hsp70, but neither Bag-4 nor Hsp40, served as a recognition site for the cytolytic attack mediated by natural killer cells.

show abstract

“…NIH-PA Author Manuscript NIH-PA Author Manuscript proteins 15 . Less well known is the fact that major surgery can also have an immunosuppressive effect that leads to a significant diminution of lymphocyte and natural killer (NK) cell function 16 .…”

Section: Nih-pa Author Manuscriptmentioning

confidence: 99%

Photodynamic therapy and anti-tumour immunity

2006

View full text Add to dashboard Cite

Photodynamic therapy (PDT) uses non-toxic photosensitizers and harmless visible light in combination with oxygen to produce cytotoxic reactive oxygen species that kill malignant cells by apoptosis and/or necrosis, shut down the tumour microvasculature and stimulate the host immune system. In contrast to surgery, radiotherapy and chemotherapy that are mostly immunosuppressive, PDT causes acute inflammation, expression of heat-shock proteins, invasion and infiltration of the tumour by leukocytes, and might increase the presentation of tumour-derived antigens to T cells.The principle of photodynamic therapy (PDT) was first proposed over 100 years ago 1 . A recent review in Nature Reviews Cancer by Rakesh Jain and colleagues described some of the historical milestones in the development of PDT as a cancer treatment2. Many of the photosensitizers (PSs) that have been studied since PDT was first proposed are based on a porphyrin-like nucleus 3 . PSs function as catalysts when they absorb visible light and then convert molecular oxygen to a range of highly reactive oxygen species (ROS). The ROS that are produced during PDT have been shown to destroy tumours by multifactorial mechanisms4 , 5 (FIG. 1). PDT has a direct affect on cancer cells, producing cell death by necrosis and/or apoptosis 6 . PDT also has an affect on the tumour vasculature, whereby illumination and ROS production causes the shutdown of vessels and subsequently deprives the tumour of oxygen and nutrients 7,8 . Finally, PDT also has a significant effect on the immune system 9-11 , which can be either immunostimulatory or immunosuppressive.Most of the commonly used cancer therapies are immunosuppressive. Chemotherapy and ionizing radiation delivered at doses sufficient to destroy tumours are known to be toxic to the bone marrow, which is the source of all cells of the immune system, and neutropaenia and other forms of myelosuppression are often the dose-limiting toxicity of these therapies. However, it should be noted that low doses of either ionizing radiation12 , 13 or chemotherapy14 can have immunostimulatory effects, including the induction of heat-shock © 2006 Nature Publishing Group Correspondence to M.R.H. Hamblin@helix.mgh.harvard.edu. Competing interests statementThe authors declare no competing financial interests. DATABASES NIH Public Access Author ManuscriptNat Rev Cancer. Author manuscript; available in PMC 2010 September 6. Published in final edited form as:Nat Rev Cancer. 2006 July ; 6(7): 535-545. doi:10.1038/nrc1894. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript proteins 15 . Less well known is the fact that major surgery can also have an immunosuppressive effect that leads to a significant diminution of lymphocyte and natural killer (NK) cell function 16 . The ideal cancer therapy would not only destroy the primary tumour, but at the same time trigger the immune system to recognize, track down and destroy any remaining tumour cells, be they at or near the site of the primary tumour or distant microm...

show abstract

Gamma Irradiation Increases hsp-70 in Chinese Hamster Ovary Cells

Cited by 59 publications

References 29 publications

Effect of gamma radiation on heat shock protein expression of four foodborne pathogens

Effect of gamma radiation on heat shock protein expression of four foodborne pathogens

Dual function of membrane-bound heat shock protein 70 (Hsp70), Bag-4, and Hsp40: protection against radiation-induced effects and target structure for natural killer cells

Photodynamic therapy and anti-tumour immunity

Contact Info

Product

Resources

About