Gamma-irradiated onions as a biological indicator of radiation dose

Vaijapurkar, S.G.; Agarwal, Deepshikha; Chaudhuri, Suhnrita; Senwar, K.R.; Bhatnagar, P. K.

doi:10.1016/s1350-4487(01)00246-3

Cited by 20 publications

(3 citation statements)

References 3 publications

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“…MH seems to affect the biosynthetic activity of the nucleolus and may also act as an inhibitor of the synthesis of nucleic acids and enzymes that are involved in the mitotic spindle, causing multipolar anaphases, lagging chromosomes and chromosome breaks (Marcano et al , 2004). Many studies on mutagen genotoxicity are now based on the scoring of micronuclei in plants (Vaijapurkar et al , 2001; Misik et al , 2016) and mammals (Avlasevich et al , 2006; Lau et al , 2009; Hashimoto et al , 2010). First proposed by Evans et al (1959), the MN test is a standard assay, which is widely used to screen for potential genotoxic agents in vitro and in vivo .…”

Section: Introductionmentioning

confidence: 99%

Dissecting the chromosomal composition of mutagen-induced micronuclei inBrachypodium distachyonusing multicolour FISH

et al. 2018

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mcFISH with chromosome-specific BAC clones offers insight into micronuclei composition, in so far as it allows their origin and formation to be determined more specifically. A reliable assay for micronuclei composition is crucial for the development of modern genotoxicity tests using plant cells. The combination of mutagenic treatments and well-developed cytomolecular resources in Brachypodium make this model species very promising for plant mutagenesis research.

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Section: Introductionmentioning

confidence: 99%

Dissecting the chromosomal composition of mutagen-induced micronuclei inBrachypodium distachyonusing multicolour FISH

et al. 2018

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“…They are mostly valued for aroma flavor and nutritional as minerals and trace elements [6,7]. One method of the postharvest cultivar is gamma-irradiated [8]. It is the most popular method but the root length of an onion guest is grown.…”

Section: Introductionmentioning

confidence: 99%

Inhibition Activity of Cleome Species Extract on Onion Guest Root

2019

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Objective: Biological control is useful in agricultural. Allelopathy plays an important factor in crop productivity. The effect of allelopathy is able to produce and release allelochemicals or phytochemicals to inhibit or stimulate seed germination, seeding growth, shoot and root growth of other crops. The aim of this study was to evaluate the inhibitory activity of Cleome viscose and Cleome spinosa extracts on onion guest roots length.Methods: The whole plants of C. viscose and C. spinosa were extracted with dichloromethane, ethyl acetate, methanol and distilled water, respectively. Each group of onion guests was sprayed at various concentrations as 100, 200, 400, 800 and 1600 parts per million for the treatment. The root growth was measured every day for 1 month.Results: At concentration of 1600 ppm, methanol crude extract of C. spinose showed the highest percent inhibitory activity value of 91.68. Ethyl acetate and methanol crude extracts of C. viscose showed strong inhibitory activity with percentage values of 90.17 and 90.90, respectively, at concentration of 1600 ppm. Moreover, the methanol extract of C. viscose and C. spinosa evaluated higher inhibitory activity than other solvent. However, distilled water crude extract of C. spinose showed weak inhibitory activity with the percentage value of 40.05 at concentration of 1600 ppm.Conclusions: In this study, the methanol crude extract of C. spinose showed potent inhibitory activity on root growth. Moreover, ethyl acetate and methanol crude extracts of C. viscose evaluated strong inhibitory activity. C. viscose and C. spinosa extracts possessed allelochemicals for postharvest biology and technology in plants.

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“…1, 2 ), onion root growth inhibition was recorded several days after γ-irradiation with very low doses, starting from 1.0 Gy. Vaijapurkar et al [ 9 ] observed changes in the growth parameters of onion bulbs ( Allium cepa ) 5 days after irradiation with high doses (more than 5 Gy), while the difference in cytogenetic parameters (mitotic index and number of micronuclei) from the control appeared 2 days later at lower doses of 2–4 Gy. In some studies, the observed effects of inhibition of plant growth parameters after irradiation were explained by cytogenetic disorders and DNA damage [ 12 , 16 ].…”

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Effect of Gamma Radiation on Cytogenetic and Growth Endpoints of Allium cepa Seedlings in Long-Term Experiments

Bolsunovsky

Трофимова

Zueva

et al. 2022

Dokl Biochem Biophys

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The effect of γ-radiation on the growth and cytogenetic endpoints of Allium cepa L. seedlings in a long period after irradiation in absorbed doses from 1.0 to 10.7 Gy were examined. The chromosome aberration rate was most sensitive to the radiation: it increased immediately after exposure at all doses. In the recovery period (up to 216 h) after irradiation, the chromosome aberration frequency naturally decreased but at the end of experiment in maximum doses remained above the control values. The impact of the initial level of chromosome aberrations on the inhibition of onion roots growth in the long terms after irradiation is discussed.

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Gamma-irradiated onions as a biological indicator of radiation dose

Cited by 20 publications

References 3 publications

Dissecting the chromosomal composition of mutagen-induced micronuclei inBrachypodium distachyonusing multicolour FISH

Dissecting the chromosomal composition of mutagen-induced micronuclei inBrachypodium distachyonusing multicolour FISH

Inhibition Activity of Cleome Species Extract on Onion Guest Root

Effect of Gamma Radiation on Cytogenetic and Growth Endpoints of Allium cepa Seedlings in Long-Term Experiments

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