Gamma interferon-producing CD4 T-cells correlate with resistance to Mycoplasma mycoides subsp. mycoides S.C. infection in cattle

Dedieu, Laurence; Balcer-Rodrigues, V.; YaYa, Aboubakar; Hamadou, B.; Cissé, Ousmane; Diallo, Mamadou Bobo; Niang, Mamadou

doi:10.1016/j.vetimm.2005.04.011

Cited by 38 publications

(56 citation statements)

References 32 publications

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“…Cultures were as previously described [5]. Heatinactivated MmmSC were obtained after one hour incubation at 60 °C.…”

Section: Mmmsc Strain and Growth Conditions And Antigen Preparationmentioning

confidence: 99%

“…LN cells were prepared as previously described [5]. One millilitre (2.5 × 10 6 cells/ mL) was then distributed into each well of a 24-well microtitre plate and incubated with heat-inactivated MmmSC (5 µg/mL), concanavalin A (2.5 µg/mL; Sigma) as a positive control to verify cell viability or in RPMI media (negative control), for 5 days at 37 °C with 5% CO 2 .…”

Section: Lymphoproliferation Assays and Cell Phenotypingmentioning

confidence: 99%

“…The cell phenotyping was performed by immunofluorescence staining, as previously described [5], using specific mouse monoclonal antibodies (MAb) to the following bovine leukocyte antigens: WC1 (CC15, SEROTEC, Cergy St. Christophe, France), CD2 (IL-A26), CD4 (IL-A12), CD8 (IL-A105), major histocompatibility complex class II (MHCII) molecules (J11), interleukin-2 receptor CD25 (IL-A111) [1,14,16,18]. Except for the former, all other MAbs were from the International Livestock Research Institute (ILRI, Nairobi, Kenya).…”

Section: Lymphoproliferation Assays and Cell Phenotypingmentioning

confidence: 99%

“…MAb DU 2 -104, kindly provided by W. Hein, was used to detect the B-cells [17]. Flow cytometry analysis, measurement of blastogenesis, of the various cell subsets and of their state of activation (CD25 expression) have already been described [5].…”

Section: Lymphoproliferation Assays and Cell Phenotypingmentioning

confidence: 99%

“…Furthermore, a recent study of the MmmSC-induced peripheral blood response revealed the predominant contribution of MmmSC-specific IFNγ-secreting CD4 T-cells to protection against CBPP [5].…”

Section: Introductionmentioning

confidence: 99%

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Characterisation of the lymph node immune response followingMycoplasma mycoidessubsp.MycoidesSC infection in cattle

et al. 2006

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“…Cultures were as previously described [5]. Heatinactivated MmmSC were obtained after one hour incubation at 60 °C.…”

Section: Mmmsc Strain and Growth Conditions And Antigen Preparationmentioning

confidence: 99%

Section: Lymphoproliferation Assays and Cell Phenotypingmentioning

confidence: 99%

Section: Lymphoproliferation Assays and Cell Phenotypingmentioning

confidence: 99%

Section: Lymphoproliferation Assays and Cell Phenotypingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterisation of the lymph node immune response followingMycoplasma mycoidessubsp.MycoidesSC infection in cattle

et al. 2006

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Contagious Bovine Pleuropneumonia

Thiaucourt¹,

Nwankpa²,

Amanfu³

2021

Veterinary Vaccines

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The protection rate of T1/44 and T1sr vaccines assessed in two independent vaccine trials varied between 33 and 67% (Thiaucourt et al., 2000). Animals were challenged against local pathogenic strains, 3 months after single vaccination. Further experiments demonstrated that T1/44 confers longer immunity than T1sr after a single dose, while a booster vaccination received 12 months after initial immunisation increased the efficacy of both vaccines (> 80%; Wesongaet al., 2000). In order to be effective vaccines must contain at least 10 7 CFU/dose of live Mmm (WOAH, 2021).Both vaccines confer low efficacy at primo-vaccination and short-term immunity, requiring re-vaccinations every 6 and 12 months respectively for T1sr and T1/44. T1/44 has residual virulence, which results in occasional cases of severe inflammation at the site of injection called Willems' reactions. These local reactions can evolve into systemic disease and may even result in death of the animals in the absence of antibiotic treatments, which can discourage vaccine acceptance by farmers . Willems' reactions are observed mainly upon first vaccination and with low occurrence after re-vaccination (Muuka et al., 2014). No side effects have been reported for T1sr.Both T1/44 and T1sr are currently produced as freeze-dried live vaccines with cold chain requirements until delivery. After reconstitution, delivery time is restricted to 30 min -2 hrs. In field conditions and with hot temperatures, viability rapidly drops below the minimum recommended titre affecting vaccine efficacy.The quality of CBPP vaccines is known to differ among producers / batches, which may result in variable vaccine efficacy. Due to the potential side effects and cold chain requirements, vaccine delivery should be restricted to fully trained veterinary personnel. GAPS :Vaccine safety: Side effects associated with the T1/44 strain are perceived as an important issue. For example, T1/44 is not accepted in some countries due to reported adverse reactions and T1sr is used instead, although the frequency of vaccination campaigns is normally once per year. Vaccine delivery can exacerbate side effects, since applying inappropriate administration routes (intradermal or intramuscular instead of subcutaneous) or using inappropriate needle gauge, etc. can result in increased adverse reactions and, thus, reduced acceptability by farmers.Vaccine efficacy: The true efficacy of T1 vaccines in the field is not well known. Control programs based on vaccination alone are not sufficient for CBPP eradication in the absence of additional countermeasures such as animal movement control. For ex.: Northern Namibia and Western Zambia, where CBPP mass vaccination campaigns are delivered annually, are still struggling to control the disease, with many outbreaks declared in 2021 and 2022.Vaccine delivery: Fully trained personnel is needed to manage vaccination, since administration problems such as use of wrong diluents and vial sizes, inappropriate number of doses per vial, etc. can result in reduced viability a...

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Increase of cells expressing PD‐1 and PD‐L1 and enhancement of IFN‐γ production via PD‐1/PD‐L1 blockade in bovine mycoplasmosis

Goto

Konnai

Okagawa

et al. 2017

Immunity Inflam & Disease

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IntroductionBovine mycoplasma, chiefly Mycoplasma bovis, is a pathogen that causes pneumonia, mastitis, arthritis, and otitis media in cattle. This pathogen exerts immunosuppressive effects, such as the inhibition of interferon production. However, the mechanisms involved in bovine mycoplasmosis have not been fully elucidated. In this study, we investigated the role of the programmed death‐1 (PD‐1)/programmed death‐ligand 1 (PD‐L1) pathway in immunosuppression in bovine mycoplasmosis.MethodsIn the initial experiments, we used enzyme‐linked immunosorbent assay to measure interferon‐γ (IFN‐γ) from peripheral blood mononuclear cells (PBMCs) isolated from cattle with mycoplasmosis.ResultsExpectedly, IFN‐γ production significantly decreased in cattle with mycoplasmosis compared with that in clinically healthy cattle. Concomitantly, flow cytometric analysis revealed that the proportions of PD‐1+CD4+ and PD‐L1+CD14+ cells significantly increased in peripheral blood of the infected cattle. Interestingly, the number of PD‐1+CD4+ and PD‐1+CD8+ T cells were negatively correlated with IFN‐γ production from PBMCs in bovine mycoplasmosis. Additionally, blockade of the PD‐1/PD‐L1 pathway in vitro by anti‐bovine PD‐1‐ and anti‐bovine PD‐L1 antibodies significantly upregulated the production of IFN‐γ from anti‐mycoplasma‐specific cells.ConclusionsThese results suggest that the PD‐1/PD‐L1 pathway could be involved in immune exhaustion of bovine mycoplasma‐specific T cells. In conclusion, our study opens up a new perspective in the therapeutic strategy for bovine mycoplasmosis by targeting the immunoinhibitory receptor pathways.

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Gamma interferon-producing CD4 T-cells correlate with resistance to Mycoplasma mycoides subsp. mycoides S.C. infection in cattle

Cited by 38 publications

References 32 publications

Characterisation of the lymph node immune response followingMycoplasma mycoidessubsp.MycoidesSC infection in cattle

Characterisation of the lymph node immune response followingMycoplasma mycoidessubsp.MycoidesSC infection in cattle

Contagious Bovine Pleuropneumonia

Increase of cells expressing PD‐1 and PD‐L1 and enhancement of IFN‐γ production via PD‐1/PD‐L1 blockade in bovine mycoplasmosis

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