Gamma Interferon Inhibits Production of Anti-OspA Borreliacidal Antibody In Vitro

Munson, Erik; Chateau, Brian K. Du; Jensen, Jani R.; Callister, Steven M.; DeCoster, David J.; Schell, Ronald F.

doi:10.1128/cdli.9.5.1095-1101.2002

Cited by 6 publications

(12 citation statements)

References 41 publications

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“…Significant IgG titers were measured in 6Ag-or Ribiϩ6Ag-immunized mice; however, the addition of IFN-␥ to the Ribiϩ6Ag preparation resulted in a significant decrease in the IgG titers of 85B, CFP21, and Mtb8.4. The ability of IFN-␥ to inhibit antibody production was also reported in Borrelia burgdorferi infection both in vitro and in vivo (37,38) and is possibly due to its ability to prevent the expression of IL-4 functions, a well-known B-lymphocyte stimulator, thus impairs the generation of a strong humoral response (40). In contrast to 85B, CFP21, and Mtb8.4 antigens, the specific IgG titers against antigen 38kDa were consistently high in all immunization protocols.…”

Section: Discussionmentioning

confidence: 94%

Gamma Interferon and Monophosphoryl Lipid A-Trehalose Dicorynomycolate Are Efficient Adjuvants forMycobacterium tuberculosisMultivalent Acellular Vaccine

2005

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In this study, we examined the immunogenicity and protective efficacy of six immunodominant Mycobacterium tuberculosis recombinant antigens (85B, 38kDa, ESAT-6, CFP21, Mtb8.4, and 16kDa) in a multivalent vaccine preparation (6Ag). Gamma interferon (IFN-␥) and monophosphoryl lipid A-trehalose dicorynomycolate (Ribi) adjuvant systems were used separately or in combination for immunization with the recombinant antigens. Our results demonstrate that immunization of mice with Ribi emulsified antigens in the presence of IFN-␥ (Ribi؉6Ag؉IFN-␥) resulted after challenge with a virulent M. tuberculosis strain in a significant reduction in the CFU counts that was comparable to that achieved with the BCG vaccine (ϳ0.9-log protection). Antigen-specific immunoglobulin G (IgG) titers in the Ribi؉6Ag؉IFN-␥-immunized mice were lower than in mice immunized with Ribi؉6Ag and were oriented toward a Th1-type response, as confirmed by elevated IgG2a levels. In addition, splenocyte proliferation, IFN-␥ secretion, and NO production were significantly higher in splenocytes derived from Ribi؉6Ag؉IFN-␥-immunized mice, whereas IL-10 secretion was decreased. These findings confirm the induction of a strong cellular immunity in the vaccinated mice that correlates well with their enhanced resistance to M. tuberculosis. The adjuvant effect of IFN-␥ was dose dependent. A dose of 5 g of IFN-␥ per mouse per immunization gave optimal protection, whereas lower or higher amounts (0.5 or 50 g/ mouse) of IFN-␥ failed to enhance protection.

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Section: Discussionmentioning

confidence: 94%

Gamma Interferon and Monophosphoryl Lipid A-Trehalose Dicorynomycolate Are Efficient Adjuvants forMycobacterium tuberculosisMultivalent Acellular Vaccine

2005

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“…In contrast, neutralization of IFN-␥ resulted in polyclonal expansion of the anti-B. burgdorferi humoral response (19). Subsequently, we showed that the borreliacidal antibody level was also augmented with effective neutralization of IFN-␥ (21).…”

mentioning

confidence: 74%

“…Figure 2 shows the results of Western immunoblotting for the detection of anti-OspA antibody with supernatants obtained from cultures of immune lymph node cells incubated with macrophages and B. burgdorferi (19) in the presence or absence of anti-IL-6 antibody or rIL-6. Supernatants obtained from immune lymph node cells incubated with macrophages and B. burgdorferi, with or without anti-IL-6, had an anti-OspA titer of 2,560 ( Fig.…”

Section: Effect Of Anti-murine Il-6 and Ril-6 On In Vitro Borreliacidmentioning

confidence: 99%

“…An attempt to augment borreliacidal activity by the addition of interleukin-4 (IL-4), a known B-lymphocyte stimulator (25), to cultures of borreliacidal antibody-producing cells was not successful (20). In addition, treatment of borreliacidal antibody-producing cells with recombinant gamma interferon (IFN-␥) also failed to promote borreliacidal activity (19). In contrast, neutralization of IFN-␥ resulted in polyclonal expansion of the anti-B.…”

mentioning

confidence: 99%

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Interleukin-6 Promotes Anti-OspA Borreliacidal Antibody Production In Vitro

Munson¹,

Nardelli²,

Luk³

et al. 2006

Clin Vaccine Immunol

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Determination of the immunological mediators responsible for promoting the production of borreliacidal antibody may facilitate the development of an improved borreliosis vaccine for human and veterinary use. Previously, we developed an in vitro assay to determine if borreliacidal antibody production could be augmented by treatment with different cytokines. In this study, in vitro treatment of lymph node cells producing borreliacidal antibody with recombinant interleukin-6 (rIL-6) resulted in a fourfold enhancement of anti-OspA borreliacidal antibody. Moreover, rIL-6 enhanced Western immunoblot titers and increased the number of B lymphocytes. In contrast, treatment of anti-OspA borreliacidal antibody-producing cells with anti-IL-6 resulted in a fourfold reduction in borreliacidal activity. Treatment with anti-IL-6 also inhibited enhanced borreliacidal antibody production induced by anti-gamma interferon. These data suggest that IL-6 plays a significant role in the production of anti-OspA borreliacidal antibodies.

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“…В развитии Лайм-артрита ведущую роль традиционно ранее приписывали Th 1 -цитокинам, таким как IFN-γ, однако в ходе недавно проведенных исследований было выявлено, что OspA B. burgdorferi может участвовать в запуске ауто-иммунного воспаления и провоцировать развитие артрита через дифференциацию Th-клеток, экс-прессирующих IL-17 [26,38]. Кроме того, в исследо-вании показано, что IFN-γ не только не индуцирует синтез анти-OspA антител, но и играет важную роль в супрессии продукции антиборрелиозных антител, что может стать обоснованием разработки высоко-эффективных вакцин [32].…”

Section: клиническая дерматология и венерология 6 2015unclassified

Erythema migrans: the cutaneous manifestation of Lyme disease

Сорокина¹,

Masyukova²

2015

Klin. dermatol. venerol.

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Gamma Interferon Inhibits Production of Anti-OspA Borreliacidal Antibody In Vitro

Cited by 6 publications

References 41 publications

Gamma Interferon and Monophosphoryl Lipid A-Trehalose Dicorynomycolate Are Efficient Adjuvants forMycobacterium tuberculosisMultivalent Acellular Vaccine

Gamma Interferon and Monophosphoryl Lipid A-Trehalose Dicorynomycolate Are Efficient Adjuvants forMycobacterium tuberculosisMultivalent Acellular Vaccine

Interleukin-6 Promotes Anti-OspA Borreliacidal Antibody Production In Vitro

Erythema migrans: the cutaneous manifestation of Lyme disease

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