Gamma-decanolactone effect on behavioral and genotoxic parameters

Viana, Carla Cristina Soares; Oliveira, Paulo Alexandre de; Brum, Lucimar Filot da Silva; Picada, Jaqueline Nascimento; Pereira, Patrı́cia

doi:10.1016/j.lfs.2006.11.042

Cited by 13 publications

(6 citation statements)

References 21 publications

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“…Therefore, the damage index could range from 0 (completely undamaged, 100 cells × 0) to 400 (with maximum damage, 100 × 4). The damage frequency (%) was calculated based on number of cells with tail versus those with no tail [27].…”

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confidence: 99%

N‐Acetylcysteine Effects on Genotoxic and Oxidative Stress Parameters in Cirrhotic Rats with Hepatopulmonary Syndrome

Vercelino¹,

Tieppo²,

Dias³

et al. 2008

Basic Clin Pharma Tox

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The aim of this study was to evaluate the potential antioxidant effects of N-acetylcysteine in hepatopulmonary syndrome, a complication of cirrhosis, using an experimental model of common bile duct ligation in rats. Male Wistar rats were divided into four experimental groups: CBDL (animals submitted to common bile duct ligation); Sham (animals submitted to simulated common bile duct ligation); Sham + N-acetylcysteine, and CBDL + N-acetylcysteine. N-acetylcysteine (10 mg/kg, intraperitoneally) was administered for 2 weeks starting on day 14 after surgery. Some alterations in the liver integrity were investigated by evaluation of serum enzymes aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and arterial blood gases. Lipoperoxidation by thiobarbituric acid-reactive substances assay, superoxide dismutase activity and total nitrates was measured as parameters of oxidative stress, performed on lung homogenates. Micronucleus assay in bone marrow and comet assay in lung, liver and blood were performed to assess the genotoxic effects by oxidative stress. The results showed an improvement in the enzymatic parameters and arterial blood gases, a reduction of lipoperoxidation and in the total nitrates after treatment with N-acetylcysteine. Histological analysis showed vasodilatation in the lung, which was reversed by N-acetylcysteine. Micronuclei frequency and DNA damage in lung and liver were increased in the CBDL group. N-Acetylcysteine caused no genotoxic effect and did not influence the induction of micronucleus in bone marrow and DNA damage in lung and liver. The results suggest protective effects after treatment with N-acetylcysteine in cirrhotic rats with hepatopulmonary syndrome.

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confidence: 99%

N‐Acetylcysteine Effects on Genotoxic and Oxidative Stress Parameters in Cirrhotic Rats with Hepatopulmonary Syndrome

Vercelino¹,

Tieppo²,

Dias³

et al. 2008

Basic Clin Pharma Tox

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“…Thirty minutes after the first administration of vehicle or PSAE, the mice were placed in the rear left square and allowed to explore the environment for 5 min (training session). Latency to start the locomotion, crossing of black lines, and rearing times were counted and used as measurements of motivation, locomotion, and exploration, respectively [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…Long-term retention of habituation to a novel environment can be considered a type of learning. A decrease in rearing between the first and second exploration sessions was considered to be a measure of habituation [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Toxicological Effects of an Aqueous Extract of Shells from the Pecan Nut Carya illinoinensis (Wangenh.) K. Koch and the Possible Association with Its Inorganic Constituents and Major Phenolic Compounds

Porto

Silva

Sousa

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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Background. Industrial processing of the pecan nut Carya illinoinensis K. Koch generated a large amount of shells, which have been used to prepare nutritional supplements and medicinal products; however, the safe use of shells requires assessment. This study evaluated the toxic, genotoxic, and mutagenic effects of pecan shell aqueous extract (PSAE) and the possible contribution of phenolic compounds, ellagic and gallic acids, and inorganic elements present in PSAE to induce toxicity. Results. Levels of inorganic elements like K, P, Cl, and Rb quantified using the Particle-Induced X-Ray Emission method were higher in PSAE than in pecan shells, while Mg and Mn levels were higher in shells. Mice showed neurobehavioral toxicity when given high PSAE doses (200–2,000 mg kg−1). The LD50 was 1,166.3 mg kg−1. However, PSAE (50–200 mg·kg−1) and the phenolic compounds (10–100 mg·kg−1) did not induce DNA damage or mutagenicity evaluated using the comet assay and micronucleus test. Treatment with ellagic acid (10–100 mg·kg−1) decreased triglyceride and glucose levels, while treatments with PSAE and gallic acid had no effect. Conclusion. Pecan shell toxicity might be associated with high concentrations of inorganic elements such as Mn, Al, Cu, and Fe acting on the central nervous system, besides phytochemical components, suggesting that the definition of the safe dose should take into account the consumption of micronutrients.

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“…Long‐term retention of habituation to a novel environmental can be considered a type of learning. The decrease in the number of rearing performed between the first and the second exploration sessions was considered as a measure of habituation .…”

Section: Methodsmentioning

confidence: 99%

“…The damage index (DI) can range from 0 (completely undamaged, 100 cells × 0) to 400 (with maximum damage, 100 × 4). Damage frequency (DF) was calculated based on the number of cells with tail versus those with no tails .…”

Section: Methodsmentioning

confidence: 99%

Neurotoxicological Profile Assessment of Lobeline after Acute Treatment in Mice

Rodrigues

Decker

Picada

et al. 2014

Basic Clin Pharma Tox

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Lobeline is a natural alkaloid with high affinity for nicotinic acetylcholine receptors, and it is a promising candidate for addiction treatment in human beings. This work evaluated the toxicological profile of lobeline with different behavioural models and investigated its effect on DNA damage (comet assay and micronucleus test) in mice. Acute administration of lobeline (5 or 10 mg/kg; i.p.) did not impair the parameters measured in the habituation and inhibitory avoidance test, suggesting that it has no effect on memory acquisition in these tasks. Lobeline did not affect the number or the latency to the first head-dip in the hole board test, indicating that it was not anxiolytic/anxiogenic in this model. No genotoxic effects were observed in blood, liver and brain tissues collected 24 hr after the single injection of lobeline (both doses). There was no increase in micronucleus frequency in mice treated with lobeline, indicating the absence of toxicity in bone marrow of the animals. Therefore, the acute treatment with high doses of lobeline did not impair the behavioural parameters measured in this work. Additionally, the drug was not able to produce DNA damage.Lobeline ( Fig. 1) is a natural alkaloid found in Lobelia inflata and has a long history of therapeutic use, as emetic and respiratory stimulant and tobacco smoking cessation agent, among other applications [1,2]. Lobeline is a nAChR ligand that has high affinity for both heteromeric and homomeric nicotinic acetylcholine receptors (nAChR), which are responsible for its antagonist as well as agonist actions [3].Some works have evaluated the effects of lobeline on behaviour. It showed anxiolytic properties and was able to inhibit the behavioural and neurochemical effects of psychostimulants in animal models [4]. Lobeline reduced voluntary ethanol drinking behaviour [5] and ethanol-induced increase in midbrain dopamine function in rodents [6]. Recently, it was reported that lobeline presents antidepressant-like effect that could be mediated in part by interaction with neuroendocrine and brain noradrenergic systems [4].Lobeline also binds and inhibits the function of vesicular monoamine transporter (VMAT) and dopamine transporter (DAT) [3,4]. Results suggest that it functions as a l-opioid antagonist. It diminishes the reinforcing properties of methamphetamine in rodents and is proposed as a treatment for nicotine and methamphetamine addiction [3].In the elevated plus-maze and marble-burying test, used as animal models to measured anxiety-like behaviour, lobeline demonstrated anxiolytic effect likely by targeting nAChRs [7].Considering the pharmacological profile of lobeline and its potential as a new therapeutic drug, the goal of this study was to investigate the toxicological profile of high lobeline doses on locomotor activity, anxiety and memory in mice. Additionally, we evaluated the DNA damage in several tissues with the comet assay and the mutagenicity in bone marrow with micronucleus test, because there are few studies on lobeline genotoxic...

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Gamma-decanolactone effect on behavioral and genotoxic parameters

Cited by 13 publications

References 21 publications

N‐Acetylcysteine Effects on Genotoxic and Oxidative Stress Parameters in Cirrhotic Rats with Hepatopulmonary Syndrome

N‐Acetylcysteine Effects on Genotoxic and Oxidative Stress Parameters in Cirrhotic Rats with Hepatopulmonary Syndrome

Evaluation of Toxicological Effects of an Aqueous Extract of Shells from the Pecan Nut Carya illinoinensis (Wangenh.) K. Koch and the Possible Association with Its Inorganic Constituents and Major Phenolic Compounds

Neurotoxicological Profile Assessment of Lobeline after Acute Treatment in Mice

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