Gamma Correction for Green Light Liquid Crystal Spatial Light Modulator and Spatial Light Interference Microscopy Imaging Study

Abstract

In recent years,quantitative phase imaging is widely used in biological tissues and cell imaging. At first, used laser or superluminescent diode is used as the light source in quantitative phase imaging. Now it mainly adopts the white light to image, as it can overcome the influence of speckle and improve the axial resolution. In this paper, the principle of low-coherence interferometry techniques is introduced. Diffractive method with 16 order binary grating is utilized to realize the green light gamma correction of the Holoeye Pluto digital electric-addressed liquid crystal spatial light modulator, and the linear relationship between phase and gray is obtained. An experiment setup is constructed (SLM), the gamma correction and the quantitative phase imaging can be completed in the same setup. The actual size of the phase ring is calculated in the 20× objective of the Olympus, and four phase grating rings are made, their phase corresponding to 0, π/2 , π and 3π/2. The four phase grating rings are loaded on the liquid crystal SLM to complete the phase modulation of the scattered light. It shows that the green light can be applied to spatical light interference microscopy (SLIM), and realizes the SLIM observation. It has certain significance for the SLIM study in the future.