Gamma BHC degradation accompanied by the growth of Clostridium rectum isolated from paddy field soil.

Abstract

A BHC decomposer isolated from a paddy field soil of Shiga prefecture was identified to be Clostridium rectum. The most dominant degradation intermediate from T-BHC was identified to be ƒÁ-3,4,5,6-tetrachlorocyclohexene (ƒÁ-TCCH) by gas chromatography-mass spectrometry. Degradation of r-BHC took place rapidly in the early growth phase of C. rectum, but was remarkably reduced in the resting state, e.g., spore-formed cell or washed cell sus

Metabolism of &gamma;-hexachlorocyclohexane by <i>Arthrobacter citreus</i> strain BI-100: Identification of metabolites

“…However, BI-100 appears to degrade g-HCH via g-PCCH to TCCH, which is then transformed to other metabolites. But the fragmentation pattern obtained for TCCH produced by BI-100 was found to be different in certain respects from that reported for g-3,4,5,6-TCCH produced by Clostridium rectum (Ohisa and Yamaguchi, 1978), indicating structural differences. It may be mentioned that four positional isomeric structures of TCCH are possible to be produced.…”

“…3). However, the fragmentation pattern of the compound obtained was found to be different in certain respects from that of g-3,4,5,6-TCCH produced by the metabolism of g-HCH by Clostridium rectum (Ohisa and Yamaguchi, 1978). It may be mentioned that four probable positional isomeric structures are possible for this compound M-2, but the isomer produced by A. citreus BI-100 (Fig.…”

“…On the contrary, an anaerobic degrader such as Clostridium sp. degrades g-HCH via g-TCCH (Ohisa and Yamaguchi, 1978). However, BI-100 appears to degrade g-HCH via g-PCCH to TCCH, which is then transformed to other metabolites.…”

“…Several reports are available on the degradation of g-HCH (Castro and Yoshida, 1971;Sethunathan, 1973;Sethunathan et al, 1983) by anaerobic bacteria such as Clostridium species and Citrobacter freundii (Jagnow et al, 1977;MacRae et al, 1969;Ohisa and Yamaguchi, 1978). The pathways of degradation of g-HCH by these bacteria have also been worked out (Heritage and MacRae, 1977a, b;Jagnow et al, 1977;Ohisa et al, 1980Ohisa et al, , 1982.…”

Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

“…There are no reports as yet on the anaerobic degradation of the ε-isomer, but it is relatively unstable anyway. Isolates capable of degrading one or more of the other four HCH isomers under anaerobic conditions include Clostridium rectum (125), Clostridium sphenoides (58,84), Clostridium butyricum, Clostridium pasteurianum (64), Citrobacter freundii (64), Desulfovibrio gigas, Desulfovibrio africanus, Desulfococcus multivorans (21), and a Dehalobacter sp. (178).…”