Gamma-Aminobutyric Acid Production from a Novel Enterococcus avium JS-N6B4 Strain Isolated from Edible Insects

Jo, Min-Ho; Hong, Seong-Jin; Lee, Ha-Nul; Ju, Jung-Hyun; Lee, Jun-Ho; Kim, Sun-Am; Eun, Jong‐Bang; Wee, Young‐Jung; Kim, Young‐Min

doi:10.4014/jmb.1905.05001

Cited by 7 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For quantitative measurement of GABA concentration produced by SH9, 10 μL of culture cell free supernatants was subjected to derivatization following the method described by Jo et al [ 29 ]. High performance liquid chromatography (HPLC) analysis was carried out for the derivatized samples using an Agilent 1260 series.…”

Section: Anti-inflammatory Effectmentioning

confidence: 99%

Psychobiotic Potential of Gamma-Aminobutyric Acid–Producing Marine Enterococcus faecium SH9 from Marine Shrimp

Sakkaa

Zaghloul

Ghanem

2022

Probiotics & Antimicro. Prot.

View full text Add to dashboard Cite

Psychobiotics are a novel class of probiotics with potential to confer mental wellness via production of neuroactive compounds such as gamma-aminobutyric acid (GABA). The demand for new biological sources of GABA has increased steadily. Therefore, the current study reports the isolation of 17 presumptive lactic acid bacteria (LAB) from marine samples and their screening for GABA synthesis from monosodium glutamate (MSG) using thin-layer chromatography (TLC). The isolate SH9 was selected as a high GABA producing strain. The GABA content of SH9 cell free supernatant (CFS) was quantitatively determined by high performance liquid chromatography (HPLC) to be 0.97 g/L. SH9 was identified biochemically and molecularly as Enterococcus faecium (identity 99%). Moreover, SH9 demonstrated promising probiotic potentials; it gave no signs of hemolysis and could survive at low pH values and high bile salt concentrations. It also exhibited antimicrobial activity against highly pathogenic strains and the ability to grow at 6.5% NaCl. In addition, SH9 CFS showed anti-inflammatory and antioxidant properties. The glutamate decarboxylase (GAD) gene was detected in SH9 by using specific primers. Product of 540 bp was obtained, sequenced, and analyzed (accession number: MW713382). The inferred amino acid sequence was 99.3% identical to Lactobacillus plantarum M-6 gadB gene. The findings of this study suggest that the marine isolate E. faecium SH9 could be used as a novel psychobiotics in the development of GABA rich healthy products.

show abstract

Section: Anti-inflammatory Effectmentioning

confidence: 99%

Psychobiotic Potential of Gamma-Aminobutyric Acid–Producing Marine Enterococcus faecium SH9 from Marine Shrimp

Sakkaa

Zaghloul

Ghanem

2022

Probiotics & Antimicro. Prot.

View full text Add to dashboard Cite

show abstract

“…In this organ, they can convert dietary glutamate to GABA, thereby providing health benefits to the host . Several Enterococcus avium strains have recently been isolated from various fermented foods, particularly East Asian fermented foods, and these strains display a high conversion rate from monosodium glutamate (MSG) to GABA, suggesting that they have the potential to be the starter organisms for GABA-rich functional food production (Tamura et al, 2010;Yang et al, 2016;Lee et al, 2017;Jo et al, 2019). Although a rare pathogen, E. avium is often present as part of the normal microbiota in the gastrointestinal tract of individuals, including infants (Birri et al, 2010;Yang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Molecular Analysis of Glutamate Decarboxylases in Enterococcus avium

Zhao

Zhang

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

Enterococcus avium (E. avium) is a common bacterium inhabiting the intestines of humans and other animals. Most strains of this species can produce gamma-aminobutyric acid (GABA) via the glutamate decarboxylase (GAD) system, but the presence and genetic organization of their GAD systems are poorly characterized. In this study, our bioinformatics analyses showed that the GAD system in E. avium strains was generally encoded by three gadB genes (gadB1, gadB2, and gadB3), together with an antiporter gene (gadC) and regulator gene (gadR), and these genes are organized in a cluster. This finding contrasts with that for other lactic acid bacteria. E. avium SDMCC050406, a GABA producer isolated from human feces, was employed to investigate the contribution of the three gadB genes to GABA biosynthesis. The results showed that the relative expression level of gadB3 was higher than those of gadB1 and gadB2 in the exponential growth and stationary phases, and this was accompanied by the synchronous transcription of gadC. After heterologous expression of the three gadB genes in Escherichia coli BL21 (DE3), the Km value of the purified GAD3 was 4.26 ± 0.48 mM, a value lower than those of the purified GAD1 and GAD2. Moreover, gadB3 gene inactivation caused decreased GABA production, accompanied by a reduction in resistance to acid stress. These results indicated that gadB3 plays a crucial role in GABA biosynthesis and this property endowed the strain with acid tolerance. Our findings provided insights into how E. avium strains survive the acidic environments of fermented foods and throughout transit through the stomach and gut while maintaining cell viability.

show abstract

Revealing the optimal traditional processing methods and its protective effects against febrile seizures of Arisaema cum bile

Zhang,

Wu,

Wang

et al. 2024

Biomedical Chromatography

View full text Add to dashboard Cite

Arisaema cum bile (known as Dan Nanxing in Chinese, DNX) is a herbal medicine used for treating febrile seizure (FS), which commonly prepared by using Arisaematis Rhizoma and animal bile. This study was designed to explore the optimal processing time of DNX and its potential mechanism on the anti‐FS effect. A total of 17 volatile organic compounds (VOCs) were the characteristic ones to distinguish different fermentation stages of DNX by using gas chromatography–ion mobility spectrometry (GC‐IMS), such as 2‐heptanone monomer, and heptanal monomer. DNX with fermentation for 3 months had an obvious pattern of VOCs with others, which could be regarded as the optimal fermentation time. The Enterococcus and Staphylococcus might be the core bacteria on the production of VOCs. Additionally, DNX (2.8 g/kg, p.o.) reversed hot water bath‐induced FSs of rats, as indicated by increased seizure latency and decreased seizure duration time. It also prevented hippocampal neuronal loss, increased GABAAR, and decreased GRIA1 expression. At the genus level, relative abundance of Enterococcus and Akkermansia were enriched after DNX treatment. These findings suggested that fermentation for 3 months might be the optimal process time for DNX, and DNX possess an anti‐FS effect through regulating neurotransmitter disorder and gut microbiota.

show abstract

Gamma-Aminobutyric Acid Production from a Novel Enterococcus avium JS-N6B4 Strain Isolated from Edible Insects

Cited by 7 publications

References 28 publications

Psychobiotic Potential of Gamma-Aminobutyric Acid–Producing Marine Enterococcus faecium SH9 from Marine Shrimp

Psychobiotic Potential of Gamma-Aminobutyric Acid–Producing Marine Enterococcus faecium SH9 from Marine Shrimp

Molecular Analysis of Glutamate Decarboxylases in Enterococcus avium

Revealing the optimal traditional processing methods and its protective effects against febrile seizures of Arisaema cum bile

Contact Info

Product

Resources

About