Gametophytic Self-Incompatibility in Petunia

Sims, Thomas L.; Robbins, Timothy P.

doi:10.1007/978-0-387-84796-2_5

Cited by 15 publications

(11 citation statements)

References 97 publications

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“…Our mathematical models (Fig. 5) suggests that 16-20 SLFs on each haplotype would be adequate to recognize the vast majority of about 40 S-RNase targets 4 .…”

Section: Discussionmentioning

confidence: 96%

“…The number of SLF types is much less than that of predicted S-RNase alleles (40 or more) 4 , thus one-to-one interactions between a SLF type and a S-RNase allele is not possible. Rather, some SLF types should interact with multiple S-RNase allelic variants, while some S-RNases can be recognized by multiple SLF types.…”

Section: Mathematical Models Suggest That 16-20 Slfs Would Be Adequatmentioning

confidence: 99%

“…A simple mathematical model showed that 16-20 SLF sequences would be adequate to recognize the vast majority of target S-RNases. We found evidence of gene conversion events, which we suggest are essential to constitute a non-self recognition system and as well as contributed to self-compatible mutations.SI is a genetically controlled reproductive barrier in angiosperms that allows the pistil to reject self (genetically-related) pollen and accept non-self (genetically-unrelated) pollen [1][2][3][4] . In most cases, this self/non-self discrimination is controlled by male-and…”

mentioning

confidence: 99%

“…SI is a genetically controlled reproductive barrier in angiosperms that allows the pistil to reject self (genetically-related) pollen and accept non-self (genetically-unrelated) pollen [1][2][3][4] . In most cases, this self/non-self discrimination is controlled by male-and…”

mentioning

confidence: 99%

“…In these families, pistil-S is a secreted ribonuclease termed S-RNase, which exerts cytotoxic effects that inhibit the elongation of self-pollen tubes by degrading RNA [1][2][3][4] ; consequently, the SI system in these families is referred to as S-RNase-based SI. The pollen-S is a set of F-box protein(s), termed S-locus F-box (SLF [1][2][3][4] , also called S-haplotype-specific F-box, SFB 6 , or S-haplotype-specific F-box brothers, SFBB 7 in Rosaceae), and function as a component of the SCF (Skp1-Cullin1-F-box)-type E3 ubiquitin ligase which generally mediates ubiquitination of target proteins for degradation by the 26S proteasome 8 . Previously, we proposed that S-RNase-based SI in Solanaceae is a collaborative non-self recognition system, in which the product of each SLF interacts with a subset of non-self S-RNases, and the products of multiple SLF types are required for the entire suite of non-self S-RNases to be collectively recognized and detoxified 9 .…”

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confidence: 99%

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Gene duplication and genetic exchange drive the evolution of S-RNase-based self-incompatibility in Petunia

et al. 2015

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Self-incompatibility (SI) systems in flowering plants distinguish self-and non-self pollen to prevent inbreeding. While other SI systems rely on the self-recognition between specific male-and femaledeterminants, the Solanaceae family has a non-self recognition system resulting in the detoxification of female-determinants of S-ribonucleases (S-RNases), expressed in pistils, by multiple male-determinants of S-locus F-box proteins (SLFs), expressed in pollen. It is not known how many SLF components of this non-self recognition system there are in Solanaceae species, or how they evolved. We identified 16-20 SLFs in each S-haplotype in SI Petunia, from a total of 168 SLF sequences using large-scale nextgeneration sequencing and genomic polymerase chain reaction (PCR) techniques. We predicted the target S-RNases of SLFs by assuming that a particular S-allele must not have a conserved SLF that recognizes its own S-RNase, and validated these predictions by transformation experiments. A simple mathematical model confirmed that 16-20 SLF sequences would be adequate to recognize the vast majority of target S-RNases. We found evidence of gene conversion events, which we suggest are essential to the constitution of a non-self recognition system and also contribute to self-compatible mutations. Self-incompatibility (SI) systems in flowering plants distinguish self and non-self pollen to prevent inbreeding. While all other SI systems studied to date rely on the self-recognition between each single male-and female-determinants, the Solanaceae plants has a non-self recognition system that functions through the detoxification of non-self female-determinants of S-ribonucleases (S-RNases), expressed in pistils, by multiple male-determinants of S-locus F-box proteins (SLFs), expressed in pollen.However, little is known about how many SLF components constitute such a non-self recognition system and how they evolve. Here we conducted large-scale next-generation sequencing and genomic PCR and identified 16-20 SLFs in each S-haplotype in SI Petunia, for a total of 168 SLF sequences. We predicted the target S-RNases of SLFs by assuming that a particular S-allele must not have a conserved SLF that recognizes its own S-RNase, and validated them by transformation experiments. A simple mathematical model showed that 16-20 SLF sequences would be adequate to recognize the vast majority of target S-RNases. We found evidence of gene conversion events, which we suggest are essential to constitute a non-self recognition system and as well as contributed to self-compatible mutations.SI is a genetically controlled reproductive barrier in angiosperms that allows the pistil to reject self (genetically-related) pollen and accept non-self (genetically-unrelated) pollen [1][2][3][4] . In most cases, this self/non-self discrimination is controlled by male-and

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“…Our mathematical models (Fig. 5) suggests that 16-20 SLFs on each haplotype would be adequate to recognize the vast majority of about 40 S-RNase targets 4 .…”

Section: Discussionmentioning

confidence: 96%

Section: Mathematical Models Suggest That 16-20 Slfs Would Be Adequatmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Gene duplication and genetic exchange drive the evolution of S-RNase-based self-incompatibility in Petunia

et al. 2015

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CRISPR/Cas9-mediated knockout of PiSSK1 reveals essential role of S-locus F-box protein-containing SCF complexes in recognition of non-self S-RNases during cross-compatible pollination in self-incompatible Petunia inflata

Sun

Kao

2017

Plant Reprod

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Function of Petunia PiSSK1. Self-incompatibility (SI), an inbreeding-preventing mechanism, is regulated in Petunia inflata by the polymorphic S-locus, which houses multiple pollen-specific S-locus F-box (SLF) genes and a single pistil-specific S-RNase gene. S -haplotype and S-haplotype possess the same 17 polymorphic SLF genes (named SLF1 to SLF17), and each SLF protein produced in pollen is assembled into an SCF (Skp1-Cullin1-F-box) E3 ubiquitin ligase complex. A complete suite of SLF proteins is thought to collectively interact with all non-self S-RNases to mediate their ubiquitination and degradation by the 26S proteasome, allowing cross-compatible pollination. For each SCF complex, the Cullin1 subunit (named PiCUL1-P) and Skp1 subunit (named PiSSK1), like the F-box protein subunits (SLFs), are pollen-specific, raising the possibility that they also evolved specifically to function in SI. Here we used CRISPR/Cas9-meditated genome editing to generate frame-shift indel mutations in PiSSK1 and examined the SI behavior of a T plant (S S ) with biallelic mutations in the pollen genome and two progeny plants (S S ) each homozygous for one of the indel alleles and not carrying the Cas9-containing T-DNA. Their pollen was completely incompatible with pistils of seven otherwise-compatible S-genotypes, but fully compatible with pistils of an S S transgenic plant in which production of S-RNase was completely suppressed by an antisense S -RNase gene, and with pistils of immature flower buds, which produce little S-RNase. These results suggest that PiSSK1 specifically functions in SI and support the hypothesis that SLF-containing SCF complexes are essential for compatible pollination.

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The self-incompatibility mating system of the olive (Olea europaea L.) functions with dominance between S-alleles

Breton

Farinelli

Shafiq

et al. 2014

Tree Genetics & Genomes

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The self-incompatibility type is of key importance to understanding pollination in orchards, because most olive cultivars are partially self-incompatible and thus require pollinizers to ensure fruit set. The gametophytic model has been advocated to function in the olive, but no allele pair has been attributed to any variety. The GSI model failed in most combinations to explain fruit set. Olive growers must screen experimentally and empirically to look for inter-compatible pair-wise combinations of varieties for optimum pollination. The sporophytic model, with given dominance relationships for six S-alleles matches 98 % of the experimental data of the two sets investigated. We propose a method to analyze data from controlled crosses between olive cultivars applied to two experiments for varieties crossed in a diallel design. Furthermore, the dominance between the S-allele pair allows rational prediction of olive variety self-incompatibility levels. The S-allele pairs were unraveled for more than 60 cultivars. To go further, crosses between reference varieties-those in which the S-allele pair was unraveled-and varieties under experimentation (VarE) with an unknown S-allele pair will enable an increase in knowledge and the choice of the best pollinizers in silico. Nevertheless, we pose outstanding questions in orchards where open-pollination efficiency with varieties harboring the R2R3, R1R3, R1R5, or R3R5 pairs. These S-allele pairs require pollen grains without R2 or R3 , R1 or R3, and R3 or R5 determinants. Such pollinizer varieties are not abundant in France and Italy, and this questions whether their spread is sufficient for optimal pollination of main varieties

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Gametophytic Self-Incompatibility in Petunia

Cited by 15 publications

References 97 publications

Gene duplication and genetic exchange drive the evolution of S-RNase-based self-incompatibility in Petunia

Gene duplication and genetic exchange drive the evolution of S-RNase-based self-incompatibility in Petunia

CRISPR/Cas9-mediated knockout of PiSSK1 reveals essential role of S-locus F-box protein-containing SCF complexes in recognition of non-self S-RNases during cross-compatible pollination in self-incompatible Petunia inflata

The self-incompatibility mating system of the olive (Olea europaea L.) functions with dominance between S-alleles

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