Gametophytic and sporophytic expression of an antherspecific <i>Arabidopsis thaliana</i> gene

Roberts, Michael R.; Foster, Gary D.; Blundell, Robert; Robinson, Susan W.; Kumar, Amar; Draper, John; Scott, Rod

doi:10.1046/j.1365-313x.1993.t01-5-00999.x

Cited by 45 publications

(3 citation statements)

References 27 publications

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“…It was reported that APG transcript was confined to anther during microspore development in Brassica. napus flower buds [87]. It was also suggested that five APG genes in Silene latifolia were related to anther fertility, which were required for development of fertile pollen [88].…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Identification, Phylogenetic and Co-Expression Analysis of OsSET Gene Family in Rice

Huang

Ouyang

et al. 2013

PLoS ONE

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BackgroundSET domain is responsible for the catalytic activity of histone lysine methyltransferases (HKMTs) during developmental process. Histone lysine methylation plays a crucial and diverse regulatory function in chromatin organization and genome function. Although several SET genes have been identified and characterized in plants, the understanding of OsSET gene family in rice is still very limited.Methodology/Principal FindingsIn this study, a systematic analysis was performed and revealed the presence of at least 43 SET genes in rice genome. Phylogenetic and structural analysis grouped SET proteins into five classes, and supposed that the domains out of SET domain were significant for the specific of histone lysine methylation, as well as the recognition of methylated histone lysine. Based on the global microarray, gene expression profile revealed that the transcripts of OsSET genes were accumulated differentially during vegetative and reproductive developmental stages and preferentially up or down-regulated in different tissues. Cis-elements identification, co-expression analysis and GO analysis of expression correlation of 12 OsSET genes suggested that OsSET genes might be involved in cell cycle regulation and feedback.Conclusions/SignificanceThis study will facilitate further studies on OsSET family and provide useful clues for functional validation of OsSETs.

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Section: Resultsmentioning

confidence: 99%

Genome-Wide Identification, Phylogenetic and Co-Expression Analysis of OsSET Gene Family in Rice

Huang

Ouyang

et al. 2013

PLoS ONE

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“…The present analysis also has reported some unexpected results. As an example, anther-specific proline-rich protein APG transcripts have been found in the pistil, when they have been considered to be confined to the anther during the period of microspore development, with a dramatic decline during pollen maturation (Roberts et al, 1993 ). This result could be explained by the implication of the proline-rich protein APG in the pollen tube during the germination process, through a process yet to be determined.…”

Section: Resultsmentioning

confidence: 99%

Identification and Functional Annotation of Genes Differentially Expressed in the Reproductive Tissues of the Olive Tree (Olea europaea L.) through the Generation of Subtractive Libraries

et al. 2017

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The olive tree is a crop of high socio-economical importance in the Mediterranean area. Sexual reproduction in this plant is an essential process, which determines the yield. Successful fertilization is mainly favored and sometimes needed of the presence of pollen grains from a different cultivar as the olive seizes a self-incompatibility system allegedly determined of the sporophytic type. The purpose of the present study was to identify key gene products involved in the function of olive pollen and pistil, in order to help elucidate the events and signaling processes, which happen during the courtship, pollen grain germination, and fertilization in olive. The use of subtractive SSH libraries constructed using, on the one hand one specific stage of the pistil development with germinating pollen grains, and on the other hand mature pollen grains may help to reveal the specific transcripts involved in the cited events. Such libraries have also been created by subtracting vegetative mRNAs (from leaves), in order to identify reproductive sequences only. A variety of transcripts have been identified in the mature pollen grains and in the pistil at the receptive stage. Among them, those related to defense, transport and oxidative metabolism are highlighted mainly in the pistil libraries where transcripts related to stress, and response to biotic and abiotic stimulus have a prominent position. Extensive lists containing information as regard to the specific transcripts determined for each stage and tissue are provided, as well as functional classifications of these gene products. Such lists were faced up to two recent datasets obtained in olive after transcriptomic and genomic approaches. The sequences and the differential expression level of the SSH-transcripts identified here, highly matched the transcriptomic information. Moreover, the unique presence of a representative number of these transcripts has been validated by means of qPCR approaches. The construction of SSH libraries using pistil and pollen, considering the high interaction between male-female counterparts, allowed the identification of transcripts with important roles in stigma physiology. The functions of many of the transcripts obtained are intimately related, and most of them are of pivotal importance in defense, pollen-stigma interaction and signaling.

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“…Most of these proteins were of unknown functions and have less than a 50% similarity (30% identity) with the cabbage protein. Among the proteins, functionally-characterized proteins include the Arabidopsis lipase Arab-1 (Brick et al, 1995), Brassica napus myrosinaseassociated proteins (Taipalensuu et al, 1997), anther-specific proline-rich proteins APG in Arabidopsis and CEX in B. napus (Roberts et al, 1993), extracellular lipase EXLs in Arabidopsis pollen coat (Mayfield et al, 2001), Medicago nodule-specific protein (Dickstein et al, 1993), Medicago pollen-specific coil protein PO22 (Wu et al, 1996), and Digitalis lanatoside O-acetylesterase (Kandzia et al, 1998). Among the bacterial enzymes that have been functionally characterized, lipases from Aeromonas hydrophila and Vibrio parahaemolyticus share homology with the cabbage protein, although the % similarity is much lower.…”

Section: Isolation Of a Chinese Cabbage Lipase-like Protein Genementioning

confidence: 99%

Characterization of a Salicylic Acid- and Pathogen-induced Lipase-like Gene in Chinese Cabbage

Lee¹,

Cho²

2003

BMB Reports

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A cDNA clone for a salicylic acid-induced gene in Chinese cabbage (Brassica rapa subsp. pekinensis) was isolated and characterized. The cabbage gene, designated Br-sil1 (for Brassica rapa salicylate-induced lipase-like 1 gene), encodes a putative lipase that has the family II lipase motif GDSxxDxG around the active site serine. A database search showed that plant genomes have a large number of genes that contain the family II lipase motif. The lipase-like proteins include a myrosinase-associated protein, an anther-specific proline-rich protein APG, a pollen coat protein EXL, and an early nodule-specific protein. The Br-sil1 gene is strongly induced by salicylic acid and a nonhost pathogen, Pseudomonas syringae pv. tomato, that elicits a hypersensitive response in Chinese cabbage. Treatment of the cabbage leaves with BTH, methyl jasmonate, or ethephon showed that the Br-sil1 gene expression is induced by BTH, but not by methyl jasmonate or ethylene. This indicates that the cabbage gene is activated via a salicylic acid-dependent signaling pathway. An examination of the tissue-specific expression revealed that the induction of the Br-sil1 gene expression by BTH occurs in leaves and stems, but not in roots and flowers. Without the BTH treatment, however, the Br-sil1 gene is not expressed in any of the tissues that were examined.

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Gametophytic and sporophytic expression of an antherspecific Arabidopsis thaliana gene

Cited by 45 publications

References 27 publications

Genome-Wide Identification, Phylogenetic and Co-Expression Analysis of OsSET Gene Family in Rice

Genome-Wide Identification, Phylogenetic and Co-Expression Analysis of OsSET Gene Family in Rice

Identification and Functional Annotation of Genes Differentially Expressed in the Reproductive Tissues of the Olive Tree (Olea europaea L.) through the Generation of Subtractive Libraries

Characterization of a Salicylic Acid- and Pathogen-induced Lipase-like Gene in Chinese Cabbage

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