Gametophytic and sporophytic expression of an antherspecific Arabidopsis thaliana gene

Abstract

Genomic and cDNA clones of the anther-specific APG gene from Arabidopsis thaliana and Brassica napus, which encodes a novel proline-rich protein, were isolated and characterized. Southern blotting and Northern analysis of male fertile and cytoplasmic male sterile varieties of B. napus showed that the APG gene is present as a single copy in the Arabidopsis genome, and that the B. napus APG gene is a member of a small anther-specific gene family. Analysis of developmentally staged B. napus flower buds indicated that APG transcript is confined to the anther during the period of microspore development. Reporter gene fusions established that the APG promoter directs expression in a number of cell types in anthers of transformed plants. This expression is consistent with the temporal pattern of mRNA accumulation in B. napus buds and follows a complex developmental pattern. Most significantly, the promoter is active in both sporophytic and gametophytic cell types, with activity of the transgene in each cell type being delineated by various cytological markers.

A Wound- and Methyl Jasmonate-Inducible Transcript Coding for a Myrosinase-Associated Protein with Similarities to an Early Nodulin

“…Also, one of the peptide sequences (pep5) was derived from a MyAP harboring the corresponding amino acid sequence. The exonl intron structure in this area appears to be conserved between the A. thaliana APG gene (Roberts et al, 1993) and the gene corresponding to MyAP4(5).…”

“…3), an early nodulin (ENOD8) from M . sativa (Dickstein et al, 1993) and an anther-specific Pro-rich protein (APG) present in both A. thaliana and B. napus (Roberts et al, 1993). MyAP and ENOD8 share 26% amino acid identity and, in addition, 26% amino acid similarity, and MyAP and the A. thaliana APG protein share 24% amino acid identity and, in addition, 25% amino acid similarity.…”

Global analysis of gene expression in flower buds of Ms-cd1 Brassica oleracea conferring male sterility by using an Arabidopsis microarray

“…Among them, At1g48020 (AtPMEI1) (Wolf et al 2003) was suppressed 11.6 fold, At2g07040 10.0 fold, Profilin 5 (At2g19770) 4.8 fold, and At2g43230 4.3 fold respectively. APG (At1g20130), which is suppressed 7.6 fold, is an antherspecific gene expressed during the period of microspore development in B. napus buds and flowers (Roberts et al 1993). MS2 (At3g11980), suppressed 5.8 fold, is expressed in tapetum of wild-type Arabidopsis flowers at, and shortly after, the release of microspores from tetrads (Aarts et al 1997).…”

Isolation and characterization of a microspore-specific gene from tobacco

“…Summarizing, genes involved in pollen development can be classified, according to expression pattern as early [dO, 28,35], and late genes [4,7,21,32,39,42]. Additionally, genes transcribed in both stages have been isolated [ 1,24,33].…”

“…A few genes have been characterized which are expressed in both developing pollen and in tapetum [24,29,33]. This is a logical consequence of an approach in which cDNA libraries were synthesized from mRNA isolated from extracts of total anther tissues.…”