Gametogenic stages in triploid oysters Crassostrea gigas: Irregular locking of gonial proliferation and subsequent reproductive effort

Jouaux, Aude; Heude-Berthelin, Clothilde; Sourdaine, Pascal; Mathieu, Michel; Kellner, K

doi:10.1016/j.jembe.2010.08.030

Cited by 66 publications

(69 citation statements)

References 28 publications

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“…Fredriksen and Sjøtun (2015) recommended using local ecotypes for crop breeding and restricting the produced crops within fjord systems and coastal areas defined by the eco-region based map catalogue from the Norwegian Environ ment Agency (Miljødirektoratet, https://kartkatalog.miljodirektoratet.no/Dataset/Details/501). Sterilization technologies for cultivated seaweeds have also been suggested as a measure to mitigate gene swamping (Loureiro et al 2015), but examples of supposedly sterile aquaculture organisms adapting over time and eventually being able to reproduce after all (Jouaux et al 2010) advise caution in such cases.…”

Section: Genetic Interactionsmentioning

confidence: 99%

Seaweed aquaculture in Norway: recent industrial developments and future perspectives

2017

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The use of cultivated seaweeds as a feedstock for multiple industrial applications has gained increasing interest in the Western World over the past decades. Norway has an extensive coastline and a well-established aquaculture sector offering suitable preconditions for developing large-scale cultivation of seaweed biomass both in monoculture and in Integrated Multi-Trophic Aquaculture (IMTA) systems. Recent efforts from research, industry and public authorities have been committed to develop a Norwegian bio-economy based on cultivated seaweed, focusing on cultivation and processing of the biomass. This review reports on the status of seaweed aquaculture in Norway, supported by production data collected since the delivery of the first commercial cultivation permits at sea in 2014. Although novel product developments are currently limited, future industrial perspectives based on cultivated biomass are being discussed. Upscaling from experimental cultivation schemes to commercial production requires a thorough assessment of the risks and benefits associated with seaweed aquaculture, as well as the development of a regulative framework adapted to this industry. Issues associated with upscaling the macroalgal production that needs to be addressed includes (i) genetic interactions between cultivated and wild crops, (ii) impacts of seaweed cultivation on surrounding ecosystems, (iii) epiphytes and diseases, (iv) area utilization and (v) threats from climate change. Addressing these issues and adapting production practices will ensure the environmental and economic sustainability of an emerging industry based on cultivated seaweed biomass in Norway.

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Section: Genetic Interactionsmentioning

confidence: 99%

Seaweed aquaculture in Norway: recent industrial developments and future perspectives

2017

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“…Gong et al (2004) reported fecundity of triploid C. gigas females as high as 13% that of diploids. More recently, Jouaux et al (2010) supposed that "un-locked" triploids, those that were able to attain near normal looking gametogenesis despite being triploid, should be considered as fertile as diploids.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…In favorable conditions, triploid oysters grow larger than diploids (Allen and Downing, 1986;Dégremont et al, 2012). Triploid oysters may also be used as a method of population control due to their sterility (Guo et al, 1996;Piferrer et al, 2009;Jouaux et al, 2010). Initially, triploids were chemically induced until Guo and Allen (1994b) developed a method to produce viable tetraploids that led to the production of so-called natural triploids (Guo et al, 1996) -a cross between diploids and tetraploids that produces 100% triploid progeny.…”

Section: Introductionmentioning

confidence: 99%

“…Although the majority of triploid oysters are effectively sterile, there are some that produce enough eggs (Allen and Downing, 1990;Jouaux et al, 2010) to use for tetraploid induction (Guo and Allen, 1994b). Cytochalasin B (CB) is a fungal antibiotic that inhibits the polymerization of actin filaments (Theodoropoulos et al, 1994) and, in shellfish zygotes, inhibits the extrusion of polar bodies, thereby creating polyploid embryos.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of cytochalasin B and 6-dimethylaminopurine for tetraploidy induction in the Eastern oyster, Crassostrea virginica

Peachey

Allen

2016

Aquaculture

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Please cite this article as: Peachey, Brittany L., Allen Jr., Standish K., Evaluation of cytochalasin B and 6-dimethylaminopurine for tetraploidy induction in the Eastern oyster, Crassostrea virginica, Aquaculture (2015), AbstractCytochalasin B (CB) has been used to induce tetraploidy in oysters since the practice began in 1993. However, CB is toxic and presents health risks to hatchery workers who administer the treatment. 6-dimethylaminopurine (6-DMAP) is also an effective cytokinetic inhibitor, and does not carry the health risks of CB. We examined the relative effectiveness of 6-DMAP vs CB for producing tetraploids in the Eastern oyster (Crassostrea virginica). Survival and yield of tetraploids varied widely among the 15 experiments. Larvae resulting from 6-DMAP treatment had higher survival in 11 of the 14 trials on day two and day six/ seven. For yield of tetraploids, 10 of 13 6-DMAP treatments had higher proportions of tetraploids on day two and at the second sampling -day six, seven, or nine -7 of 10 had higher proportions of tetraploids. Tetraploid spat were obtained from the majority of surviving cultures. Based on these results, 6-DMAP can effectively replace CB for inducing polyploidy in C. virginica, and probably other Crassostrea spp., due to the success of the treatment, the ease of application, and the reduction in health risk to hatchery workers. This study set the precedent for the use of 6-DMAP on C. virginica and A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT 2 established a new procedure for inducing tetraploids using triploid eggs. It might be possible to refine the treatment to further optimize yield of tetraploids.

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“…Furthermore, reproductive events were reported in triploid oysters considered to be sterile. An analysis a Corresponding author: clothilde.heude@unicaen.fr of different patterns of the reproductive cycle in these oysters has been described by Jouaux et al (2010). New knowledge on the first stages of the reproductive cycle in diploid oysters could serve to help us understand this gametogenesis disturbance in their triploid counterparts.…”

Section: Introductionmentioning

confidence: 99%

Male germ cells of the Pacific oysterCrassostrea gigas: flow cytometry analysis, cell sorting and molecular expression

Franco

Kellner

Mathieu

et al. 2011

Aquat. Living Resour.

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-A technique was developed for dissection and isolation of male germ cells in the oyster Crassostrea gigas.This procedure can provide cells for the exploration of processes involved in the reproductive physiology of bivalves. Spermatogonia were chosen because of their essential role in spermatogenesis and the impact of gonia proliferation on reproductive effort. A non lethal method for determining sex and reproductive cycle stage was first validated in oysters. This first step was essential in order to constitute a homogeneous pool of oysters at the same stages of gametogenesis. Germ cell fractions were then obtained from a density gradient, and enrichment of each fraction was ratified by electron microscopy and by means of a 2-parameter flow cytometry procedure (DNA and mitochondrial staining). A significant enrichment in spermatogonia and spermatocytes was confirmed by the increased expression of markers of proliferative cells (proliferative cell nuclear antigen, PCNA) and early germ cells (oyster vasa-like gene). A preliminary cell sorting procedure is also reported, which was applied to fractions enriched in spermatogonia.

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Gametogenic stages in triploid oysters Crassostrea gigas: Irregular locking of gonial proliferation and subsequent reproductive effort

Cited by 66 publications

References 28 publications

Seaweed aquaculture in Norway: recent industrial developments and future perspectives

Seaweed aquaculture in Norway: recent industrial developments and future perspectives

Evaluation of cytochalasin B and 6-dimethylaminopurine for tetraploidy induction in the Eastern oyster, Crassostrea virginica

Male germ cells of the Pacific oysterCrassostrea gigas: flow cytometry analysis, cell sorting and molecular expression

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