Gametogenesis in a dish

Gu, Ying; Liu, Guanghui; Belmonte, Juan Carlos Izpisúa

doi:10.1038/cr.2012.84

Cited by 5 publications

(4 citation statements)

References 19 publications

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“…About 10–15% of all couples are infertile, with male infertility accounting for half of these cases (Gu, Liu, & Belmonte, , Massart, Lissens, Tournaye, & Stouffs, ). About 200,000 babies are born every year using assisted reproductive technologies, which has helped many couples with fertility problems (Zegers‐Hochschild et al, ); unfortunately, patients without gametes cannot benefit from the existing approaches.…”

Section: Introductionmentioning

confidence: 99%

“…For example, normal sperm collection in 10% of infertile men with obstructive azoospermia and non‐obstructive azoospermia is not possible—especially in non‐obstructive azoospermia patients, for whom even testicular extraction of sperm is not helpful (Su et al, ). Other causes of male infertility are associated with impaired spermatogenesis due to genetic deficiency or environmental factors, or result from cancer treatments, including radiotherapy and chemotherapy (Gu et al, ). The only currently available method for treating such male infertility is through donor sperm.…”

Section: Introductionmentioning

confidence: 99%

“…radiotherapy and chemotherapy (Gu et al, 2012). The only currently available method for treating such male infertility is through donor sperm.…”

mentioning

confidence: 99%

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Insights into in vitro spermatogenesis in mammals: Past, present, future

Fattahi

Latifi

Ghasemnejad

et al. 2017

Molecular Reproduction Devel

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Considering the self-renewal and differentiation ability of pluripotent stem cells, some studies have pointed out the possibility of stem cell-derived sperm production. Most studies that test this hypothesis have been conducted on rodents, with some promising results; however, studies on humans are progressing slowly, and have encountered technical and ethical hurdles. Established methods to differentiate stem cells-including embryoid bodies, co-culturing, and various feeder cells-may provide a niche that is similar to in vivo conditions and resolve epigenetic abnormalities, but a gonadal-like three-dimensional structure is still required to produce germ cells with the correct imprinting. In the last few years, sperm-like cells with fertilizing capacity were produced from mouse embryonic stem cells, and the resulting embryos from these cells yielded live offspring. Future research should move towards the use of adult stem cells, however, owing to the unavailability of embryonic cells in adults. More intensive research and techniques are required since in vitro spermatogenesis provides hope to individuals without mature sperm who cannot be treated, and may be a useful system to study the precise mechanism of spermatogenesis. In this review, we describe recent studies of in vitro spermatogenesis mechanisms and related techniques in mammals. We also discuss the possible cell surface markers and culture conditions that might improve in vitro spermatogenesis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Insights into in vitro spermatogenesis in mammals: Past, present, future

Fattahi

Latifi

Ghasemnejad

et al. 2017

Molecular Reproduction Devel

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“…Nanocasting into mesoporous silica is also a route to mixed metal oxides. The interest here is primarily in magnetic materials such as (1) ferrites: MFe 2 O 4 where M ¼ zinc, [62] manganese, [63] nickel, [57,63,64] cobalt, [63][64][65][66] copper, [57,63] and magnesium, [64] (2) strontium hexaferrite, [67] (3) NiCo 2 O 4 spinel, [68,69] and (4) copper doped cerium oxide [57] for solid oxide fuel cell applications.…”

Section: Nanocasting To Form Other Hierarchically Porous Materialsmentioning

confidence: 99%

Formation of Hierarchically Porous Metal Oxide and Metal Monoliths by Nanocasting into Silica Monoliths

et al. 2012

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The formation of hierarchically porous metal and metal oxide monoliths by replication of hierarchically porous silica templates is reviewed. The various factors that impact the structure and properties of the synthesized materials are discussed and illustrated by the formation of new α‐Fe2O3, ZrO2, nickel, silver, and silver silicate porous monoliths. The impact of the atmosphere is addressed in the formation of Co3O4 and silver monoliths. For Co3O4, formation of the monolith under vacuum, air, argon, or nitrogen was found to dramatically change the structure of the final material. For silver, decomposition of the silver nitrate under air resulted in porous monoliths composed of silver silicates. Decomposition of silver nitrate under vacuum produced monoliths for which the chemical composition of the monolith was predominantly silver on the exterior of the monolith consisted of silver silicates in the interior of the monolith.

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