Gametocidal Factor Transferred from Aegilops geniculata Roth Can Be Adapted for Large-Scale Chromosome Manipulations in Cereals

Kwiatek, Michał; Wiśniewska, Halina; Ślusarkiewicz‐Jarzina, A.; Majka, Joanna; Majka, Maciej; Belter, Jolanta; Pudelska, H.

doi:10.3389/fpls.2017.00409

Cited by 16 publications

(32 citation statements)

References 55 publications

(61 reference statements)

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“…Since SD can affect accuracy of linkage map construction and also have breeding implications, it has been addressed in several studies involving crop species, including wheat (e.g., [47,49,[51][52][53]). In progeny of wheat-alien combinations, one of the most likely causes for the skewed transmission of complete or segmental alien chromosomes of various wild species (most studied those from Aegilops and Thinopyrum genera) has been suggested to be gametophytic competition during zygote formation, with male gametogenesis being affected in the majority, though not all of the cases [36,54,55].…”

Section: An Intriguing Issue In Wheat-alien Gene Transfer: Segregatiomentioning

confidence: 99%

Harnessing Genetic Diversity of Wild Gene Pools to Enhance Wheat Crop Production and Sustainability: Challenges and Opportunities

et al. 2017

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Wild species are extremely rich resources of useful genes not available in the cultivated gene pool. For species providing staple food to mankind, such as the cultivated Triticum species, including hexaploid bread wheat (Triticum aestivum, 6x) and tetraploid durum wheat (T. durum, 4x), widening the genetic base is a priority and primary target to cope with the many challenges that the crop has to face. These include recent climate changes, as well as actual and projected demographic growth, contrasting with reduction of arable land and water reserves. All of these environmental and societal modifications pose major constraints to the required production increase in the wheat crop. A sustainable approach to address this task implies resorting to non-conventional breeding strategies, such as "chromosome engineering". This is based on cytogenetic methodologies, which ultimately allow for the incorporation into wheat chromosomes of targeted, and ideally small, chromosomal segments from the genome of wild relatives, containing the gene(s) of interest. Chromosome engineering has been successfully applied to introduce into wheat genes/QTL for resistance to biotic and abiotic stresses, quality attributes, and even yield-related traits. In recent years, a substantial upsurge in effective alien gene exploitation for wheat improvement has come from modern technologies, including use of molecular markers, molecular cytogenetic techniques, and sequencing, which have greatly expanded our knowledge and ability to finely manipulate wheat and alien genomes. Examples will be provided of various types of stable introgressions, including pyramiding of different alien genes/QTL, into the background of bread and durum wheat genotypes, representing valuable materials for both species to respond to the needed novelty in current and future breeding programs. Challenging contexts, such as that inherent to the 4x nature of durum wheat when compared to 6x bread wheat, or created by presence of alien genes affecting segregation of wheat-alien recombinant chromosomes, will also be illustrated.

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Section: An Intriguing Issue In Wheat-alien Gene Transfer: Segregatiomentioning

confidence: 99%

Harnessing Genetic Diversity of Wild Gene Pools to Enhance Wheat Crop Production and Sustainability: Challenges and Opportunities

et al. 2017

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Section: Plant Materialsmentioning

confidence: 99%

“…In the initial study, monosomic addition triticale line (MA2S k ; 2n = 43) that carried chromosome 2S k derived from triticale-Aegilops kotschyi hybrids was used. These plants were crossed with triticale nullisomic plants for chromosomes 2R (N2R, 2n = 40); obtained Agronomy 2019, 9, 646 4 of 12 by Kwiatek et al [35]. Double monosomic (M2R and M2S k ) F 1 plants (2n = 42) were identified using GISH, according to Kwiatek et al [35].…”

Section: Plant Materialsmentioning

confidence: 99%

“…These plants were crossed with triticale nullisomic plants for chromosomes 2R (N2R, 2n = 40); obtained Agronomy 2019, 9, 646 4 of 12 by Kwiatek et al [35]. Double monosomic (M2R and M2S k ) F 1 plants (2n = 42) were identified using GISH, according to Kwiatek et al [35]. Ditelosomic (Dt2RS and Dt2RL) plants of triticale were generated by Kwiatek et al [35] by harnessing chromosome fragmentation induced by gametocidal action, in combination with the production of double haploids.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Double monosomic (M2R and M2S k ) F 1 plants (2n = 42) were identified using GISH, according to Kwiatek et al [35]. Ditelosomic (Dt2RS and Dt2RL) plants of triticale were generated by Kwiatek et al [35] by harnessing chromosome fragmentation induced by gametocidal action, in combination with the production of double haploids. Spikes emasculated between April and June of 2018 were used for cross-pollination.…”

Section: Plant Materialsmentioning

confidence: 99%

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Recovery of 2R.2Sk Triticale-Aegilops kotschyi Robertsonian Chromosome Translocations

et al. 2019

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Robertsonian translocations (RobTs) in the progeny of triticale (×Triticosecale Wittmack) plants with monosomic substitution of Aegilops kotschyi chromosome 2S k (2R) were investigated by fluorescence in-situ hybridization. Chromosome 2S k of Ae. kotschyi is reported to possess many valuable loci, such as Lr54 + Yr37 leaf and stripe (yellow) rust resistance genes. We used a standard procedure to produce RobTs, which consisted of self-pollination of monosomic triticale plants, carrying 2R and 2S k chromosomes in monosomic condition. This approach did not result in RobTs. Simultaneously, we succeeded in producing 11 plants carrying 2R.2S k compensatory RobTs using an alternative approach that utilized ditelosomic lines of triticale carrying 2RS (short arm) and 2RL (long arm) telosomic chromosomes. Identification of molecular markers linked to Lr54 + Yr37 genes in the translocation plants confirmed that these resources can be exploited in current triticale breeding programmes.Agronomy 2019, 9, 646 2 of 12 from wild relatives into the wheat genetic background [10]. Recently, several attempts were made to transfer rust resistance genes from Aegilops, Agropyron and Triticum species into triticale [11][12][13][14][15].Aegilops species are closely related to wheat (and triticale, per se) and carry a number of valuable traits, which have been effectively incorporated into wheat by developing wheat-Aegilops hybrids and deriving addition, substitution and translocation lines [16]. Aegilops kotschyi Boiss. (2n = 4x = 28 chromosomes, U-and S-genomes) is a wild tetraploid goatgrass native to Northern Africa, the Mid-East, and Western Asia. Ae. kotschyi germplasm is exploited in wheat breeding [17] as a source of high grain protein, iron and zinc [18]. Moreover, Antonov and Marais [19] observed leaf rust resistance that was effective against the infection of Puccinia triticina in Ae. kotschyi. Marais et al. [20] identified the Lr54 and Yr37 leaf rust and stripe rust resistance genes, and developed aT2DS.2S k L wheat-Ae. kotschyi translocation line. The first Lr54 + Yr37 marker was developed by Heyns et al. [21]. Moreover, translocation gene sequences were cloned and specific SSR markers were developed [22].Homoeologous recombination based engineering is the most common way for efficiently utilizing the wild relative gene pool for crop improvement [23]. The generation of translocation lines is the most promising pathway for the exploitation of alien germplasm in crop breeding [23]. In distant hybrids, unpaired chromosomes are present as univalents during meiosis. Monosomic chromosomes are prone to centric breaks at anaphase I of meiosis, which misdivide and the broken ends fuse during the interkinesis of meiosis II [24][25][26]. Fusion of the misdivided products may result in the formation of a Robertsonian translocation (RobT) [27].Several steps are required to generate RobTs (Figure 1a), with self-pollination of double-monosomic plants being the most common method used in the induction of RobTs [26]. Wheat breeders can use a l...

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Characteristic of the gene candidate SecARS encoding alkylresorcinol synthase in Secale

Targońska-Karasek,

Kwiatek,

Groszyk

et al. 2023

Mol Biol Rep

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Background Alkylresorcinols (ARs) are compounds belonging to the class of phenolic lipids. A rich source of ARs are cereal grains such as rye, wheat, triticale or barley. ARs found in plants are characterized by a variety of biological properties such as antimicrobial, antifungal and cytotoxic activity. Moreover, they are proven to have a positive influence on human health. Here, we aimed to find and characterize the gene with ARs synthase activity in the species Secale cereale. Methods and results Using BAC library screening, two BAC clones containing the gene candidate were isolated and sequenced. Bioinformatic analyses of the resulting contigs were used to examine the structure and other features of the gene, including promoter, intron, 3’UTR and 5’UTR. Mapping using the FISH procedure located the gene on the 4R chromosome. Comparative analysis showed that the gene is highly similar to sequences coding for type III polyketide synthase. The level of gene expression in various parts of the plant was investigated, and the biochemical function of the gene was confirmed by heterologous expression in yeast. Conclusions The conducted analyses contributed to a better understanding of the processes related to ARs synthesis. Although the research concerned the rye model, the knowledge gained may help in understanding the genetic basis of ARs biosynthesis in other species of the Poaceae family as well.

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Gametocidal Factor Transferred from Aegilops geniculata Roth Can Be Adapted for Large-Scale Chromosome Manipulations in Cereals

Cited by 16 publications

References 55 publications

Harnessing Genetic Diversity of Wild Gene Pools to Enhance Wheat Crop Production and Sustainability: Challenges and Opportunities

Harnessing Genetic Diversity of Wild Gene Pools to Enhance Wheat Crop Production and Sustainability: Challenges and Opportunities

Recovery of 2R.2Sk Triticale-Aegilops kotschyi Robertsonian Chromosome Translocations

Characteristic of the gene candidate SecARS encoding alkylresorcinol synthase in Secale

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