Gamete quality of streaked prochilod Prochilodus lineatus (Characiformes) after GnRHa and dopamine antagonist treatment

Abstract

The efficiency of Ovaprim™ salmon gonadotropin-releasing hormone agonist (GnRHa) and dopamine antagonist on the induction of spawning and spermiation in Prochilodus lineatus in comparison with the commonly used method using pituitary extract (PE) was evaluated. Females received PE at 0.5 + 5.0 mg/kg and Ovaprim™ at 0.05 + 0.45 ml/kg or at 0.125 + 0.375 ml/kg. All males received a first dose of PE at 0.4 mg/kg and then PE at 4.0 mg/kg or Ovaprim™ at 0.25 ml/kg. Oocyte, egg, larvae and sperm quality were evaluated. All females spawned and oocyte, egg and larvae quality were similar between Ovaprim™-treated (both doses) and PE-treated females. Data from females were pooled and the mean values were: 242 g ova weight, 15% ova index, 1209 oocytes/g ova, 284,539 oocytes/female, 183 oocytes/g body weight, 1.18 mm oocyte diameter, 49% fertilization rate, 43% hatching rate and 89% normal larvae. Sperm quality was similar between Ovaprim™-treated and PE-treated males. Data from males were pooled and the mean values of semen were: volume of 3.0 ml, 14.9 × 109 sperm/ml, osmolality of 283 mOsm/kg, pH of 7.4, 71% motile sperm, 217 μm/s curvilinear velocity, 102 μm/s straight-line velocity and 189 μm/s average path velocity. Ovaprim™ treatment can be used for commercial reproduction of P. lineatus, without any loss of gamete quality in comparison with PE treatment.

Fresh, equilibrated and post-thaw sperm quality of Brycon orbignyanus (Valenciennes, 1850) and Prochilodus lineatus (Valenciennes, 1837) treated with either salmon GnRHa and domperidone or pituitary extract

“…The total amount of straws was 54 for B. orbignyanus (three 0.25-mL straws x 3 hormone treatments x 6 males) and 63 for P. lineatus (three 0.5-mL-straws x 3 hormone treatments x 7 males). Approximately two months later, straws were thawed in a 60°C water bath (Water-bath MA 127, Marconi, Brazil) for 3 s (0.25 mL straws) or for 8 s (0.5 mL straws), and post-thaw sperm quality was estimated using the CASA system (Computer-Assisted Sperm Analyzer), following the methodology described in Viveiros et al (2013). Briefly, post-thaw sperm was activated directly in a Makler ™ counting chamber (Sefi-Medical Instruments ltd, Haifa, Israel) to a final dilution ratio of 1:50 (B. orbignyanus) or 1:500 (P. lineatus).…”

Seminal plasma features of Prochilodus lineatus and Brycon orbignyanus throughout two consecutives spawning seasons

“…The detailed description of motility features over the SS is described elsewhere (Di Chiacchio et al, ). Motility, VCL and VSL were estimated using a computer‐assisted sperm analysis system, according to Viveiros, Gonçalves, Di Chiacchio, et al (). Briefly, motility was triggered in a 100 mOsm/kg NaCl solution at a final dilution ratio (v/v) of 1 sperm: 100 NaCl directly in a Makler™ counting chamber (Sefi‐Medical Instruments Ltd, Haifa, Israel) placed under a phase contrast microscope (Eclipse E200; Nikon, Tokyo, Japan), objective magnification: ×10, ocular at ×100 magnification, with a green filter and phase one position.…”

“…Both species are from the Characiformes order, are well adapted to captivity and have a great potential for Brazilian freshwater aquaculture. They have been used in a number of studies addressing spawning/spermiation induction (Viveiros, Gonçalves, Di Chiacchio, et al, ; Viveiros, Gonçalves, Nascimento, & Leal, ) and sperm storage, and in restocking programs through artificial propagation for conservation and management in Brazil (Maria, Viveiros, Freitas, & Oliveira, 2006; Maria, Viveiros, Orfão, Oliveira, & Moraes, 2006).…”

Methyl glycol, methanol and DMSO effects on post-thaw motility, velocities, membrane integrity and mitochondrial function of Brycon orbignyanus and Prochilodus lineatus (Characiformes) sperm

“…It is interesting to observe the higher sperm concentration Table 2 Post-thaw quality (mean ± SD) of Brycon orbignyanus (n = 5 males) and Prochilodus lineatus (n = 5 males) sperm frozen in three cryoprotectants (CPA) combined with two extenders Viveiros et al 2013), while B. orbignyanus produces a low concentration and a high volume of sperm (frequently above 10 mL; Maria et al 2006b). A better knowledge of fresh sperm characteristics is necessary to evaluate sperm quality before experiments in order to guarantee high initial quality of sperm parameters.…”