Gamete Production from Stem Cells

Symosko, Krista M.; Schatten, Gerald; Easley, Charles A.

doi:10.1007/978-3-030-47767-7_32

Cited by 2 publications

(2 citation statements)

References 137 publications

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“…Recently, the first development of blastocyst was reported in non-human primates (NHP) followed by fertilization with in vitro derived spermatid from embryonic stem cells utilizing the same directed-differentiation protocol used in the human [164,165]. The differentiation protocol is being improved [166,167]. The successful generation of fertile male gametes requires a seamless orchestration between male germ cells and supporting cells including Sertoli, Leydig, and peritubular cells where spermatogenesis occurs in a highly organized tubular microenvironment, the seminiferous tubules, which constitute the mammalian testis.…”

Section: Spermatozoon As a Potential Model For Studying Hdmentioning

confidence: 99%

Spermatozoan Metabolism as a Non-Traditional Model for the Study of Huntington’s Disease

Lawlor

Zigo

Kerns

et al. 2022

IJMS

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Huntington’s Disease (HD) is a fatal autosomal dominant neurodegenerative disease manifested through motor dysfunction and cognitive deficits. Decreased fertility is also observed in HD animal models and HD male patients, due to altered spermatogenesis and sperm function, thus resulting in reduced fertilization potential. Although some pharmaceuticals are currently utilized to mitigate HD symptoms, an effective treatment that remedies the pathogenesis of the disease is yet to be approved by the FDA. Identification of genes and relevant diagnostic biomarkers and therapeutic target pathways including glycolysis and mitochondrial complex-I-dependent respiration may be advantageous for early diagnosis, management, and treatment of the disease. This review addresses the HD pathway in neuronal and sperm metabolism, including relevant gene and protein expression in both neurons and spermatozoa, indicated in the pathogenesis of HD. Furthermore, zinc-containing and zinc-interacting proteins regulate and/or are regulated by zinc ion homeostasis in both neurons and spermatozoa. Therefore, this review also aims to explore the comparative role of zinc in both neuronal and sperm function. Ongoing studies aim to characterize the products of genes implicated in HD pathogenesis that are expressed in both neurons and spermatozoa to facilitate studies of future treatment avenues in HD and HD-related male infertility. The emerging link between zinc homeostasis and the HD pathway could lead to new treatments and diagnostic methods linking genetic sperm defects with somatic comorbidities.

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Section: Spermatozoon As a Potential Model For Studying Hdmentioning

confidence: 99%

Spermatozoan Metabolism as a Non-Traditional Model for the Study of Huntington’s Disease

Lawlor

Zigo

Kerns

et al. 2022

IJMS

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“…Two recent publications have covered extensively the topic of in vitro spermatogenesis (IVS) in mice and human [12,13], and two publications on IVG [14,15]. Therefore, this review will only cover the recent developments in in vitro spermatogenesis in the past two years and focus on nonmurine studies.…”

Section: Introductionmentioning

confidence: 99%

Recent Developments in In Vitro Spermatogenesis and Future Directions

Cho,

Easley

2023

Reprod. Med.

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Recent developments in stem cell technologies have made significant advancements in the field of in vitro gametogenesis. In vitro gametogenesis (IVG) is a promising technology where functional gametes (sperm or egg cells) can be generated from stem cells. Scientists have made continuous advancements in the field and successfully derived fully functional sperm from stem cells in mice. Two recent papers generated excitement in IVG by generating bi-maternal and bi-paternal mice from embryonic stem cells (ESCs) and pluripotent stem cells (PSCs). IVG is a promising technology with potential applications that include infertility treatment, fertility preservation, same-sex reproduction, bypassing oocyte depletion in women with advanced age, conservation biology, genetic disorder prevention, and research into human germ cell development. In vitro spermatogenesis (IVS) is the attempt to recreate the process of spermatogenesis in a culture system. Spermatogenesis is essential for male fertility and reproductive health, but it can be impaired by various factors such as genetic defects, environmental toxicants, infections, aging, or medical therapies. Spermatogenesis is a complex and highly regulated process involving multiple cell proliferation, differentiation, and maturation stages. The main challenges of IVS are to provide a suitable microenvironment that mimics the testis in vivo, to support the survival and development of all the cell types involved in spermatogenesis, and to achieve complete and functional spermatogenesis. Therefore, there is a great interest in developing methods to study spermatogenesis in vitro, both for basic research and clinical applications. This review covers recent developments in in vitro spermatogenesis in the past two years. Advances in tissue engineering and regenerative medicine have introduced techniques like ex vivo tissue culture and technologies such as bioreactors, microfluidic systems, and organoids. Bioreactors and microfluidic systems replicate physiological conditions for tissue and cell cultivation, while organoids model organ functionality. Meanwhile, scaffolds, made from various materials, provide essential structural support, guiding the growth and organization of cells into functional tissues.

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Gamete Production from Stem Cells

Cited by 2 publications

References 137 publications

Spermatozoan Metabolism as a Non-Traditional Model for the Study of Huntington’s Disease

Spermatozoan Metabolism as a Non-Traditional Model for the Study of Huntington’s Disease

Recent Developments in In Vitro Spermatogenesis and Future Directions

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