Gambaran Hasil Pemeriksaan BTA dan Genexpert Pada Pasien Suspek Tuberkulosis Di RSUD Budhi Asih Jakarta Timur

Nurdiani, Catu Umirestu; Kristianingsih, Yuli; Zahrawani, Aqilah Qisthi

doi:10.37012/anakes.v8i1.873

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hasil penelitian skala besar menunjukkan bahwa pemeriksaan TCM mempunyai sensitivitas dan spesifisitas yang lebih baik dibandingkan pemeriksaan mikroskopis. Akan tetapi, pemeriksaan metode TCM ini terbatas hanya untuk pendeteksian resistansi terhadap rifampisin (Affiyanti et al, 2023).…”

Section: Pendahuluanunclassified

Optimization of DNA amplification temperature in quantitative polymerase chain reaction for Identification of isoniazid-resistant Mycobacterium tuberculosis

Endarwati,

Indra,

Hardiana

et al. 2024

curr biomed

View full text Add to dashboard Cite

Background: Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis and is a serious threat to global health. The methods can be used to detect and identify the bacteria is quantitative polymerase chain reaction (qPCR). In this method, denaturation and extension temperatures are determining factors of success that needs to be optimized. Objective: This study aims to optimize denaturation and extension temperatures in M. tuberculosis DNA amplification. Methods: The research used quasi-experimental design. The denaturation temperature optimized were 93, 94, 95, 96, and 97°C, and the extension temperature optimized were 58, 59, 60, 61, and 62°C. The test sample was a 1 ml sputum sample isolated from a patient with isoniazid-resistant M. tuberculosis. Optimization was performed using seven test primers, namely S315T, S315N, S315I, S315R, S315G, S315L, and R463B with the katG gene target and data analysis using Ms Excel. Data optimization results were processed with Excel by taking the lowest Ct value. Results: The results showed that the optimization temperatures for denaturation were different for each primer used. Primers S315T, S315R, and S315G, optimal with denaturation temperature of 96°C, primer S315N optimal with 94°C, primers S315I and R463B optimal with 93°C, and for primer S315L optimal with 95°C, with the most widely used temperature is 96°C. The optimal extension temperature was 58°C for primers S315T, S315N, S315I, and R463B, at 60°C for primers S315R and S315G, and at 61°C for primer S315L. Conclusion: The optimal denaturation temperature in this study was 96°C and the optimal extension temperature was 58°C.

show abstract

Section: Pendahuluanunclassified

Optimization of DNA amplification temperature in quantitative polymerase chain reaction for Identification of isoniazid-resistant Mycobacterium tuberculosis

Endarwati,

Indra,

Hardiana

et al. 2024

curr biomed

View full text Add to dashboard Cite

show abstract

“…The abundance of mucus will increase the sample volume, reducing the likelihood of obtaining a sample containing M. tuberculosis. On the other hand, TCM examination with molecular detection can qualitatively detect DNA from the MTB complex but cannot be used as a follow-up examination (monitoring) in patients undergoing treatment (Nurdiani et al, 2022;Kementerian Kesehatan Republik Indonesia, 2017).…”

Section: Conclusion and Recommendationsmentioning

confidence: 99%

Comparison of Tuberculosis Examination Using Ziehl-Neelsen Method and Molecular Rapid Test

Anwar,

Handayani,

Nurdin

et al. 2023

JKM

View full text Add to dashboard Cite

Tuberculosis is an infectious disease of the respiratory tract caused by infection with the bacteria Mycobacterium tuberculosis. Fast and accurate diagnostic methods are very important for diagnosing and controlling pulmonary TB disease in Indonesia. TB examination using the Zn method is very commonly used even though it has lower sensitivity than TCM. The aim of this study was to compare the results of TB examination using BTA staining with the Molecular Rapid Test. This type of research uses analytical observational methods with a cross-sectional approach. The sample was 40 respondents taken using purposive sampling technique. The results of tuberculosis examination using the Ziehl-Neelsen (ZN) BTA staining method and the Moluker rapid test tool (TCM) method showed that the microscopic results of the Ziehl-Neelsen method of BTA smear staining showed negative results in 31 people (75%), 1 respondent (2 .5%) with scanty results and 9 respondents (22.5%) with 1+ results. The results of the TB BTA examination using the TCM method resulted in MTB Not Detected with a total of 30 people (75%), MTB Detected Medium obtained a result of 6 people (15%), MTB Detected Low obtained a result with a total of 3 people (7.5%), MTB Detected Very Low results were obtained with the number of 1 person (2.5%) and in the SPSS results using the t-test the results obtained were p value <0.05 (0.012 <0.05) which means there is a comparison of the BTA staining tuberculosis examination using the Ziehl-Ziehl method. neelsen and Molecular Rapid Tests. Based on the research results, it was found that there was a significant difference between the Ziehl-Neelsen BTA staining tuberculosis examination method and the Molecular Rapid Test Tool with a p value <0.05 (0.012 <0.05)

show abstract

Gambaran Hasil Pemeriksaan BTA dan Genexpert Pada Pasien Suspek Tuberkulosis Di RSUD Budhi Asih Jakarta Timur

Cited by 2 publications

References 0 publications

Optimization of DNA amplification temperature in quantitative polymerase chain reaction for Identification of isoniazid-resistant Mycobacterium tuberculosis

Optimization of DNA amplification temperature in quantitative polymerase chain reaction for Identification of isoniazid-resistant Mycobacterium tuberculosis

Comparison of Tuberculosis Examination Using Ziehl-Neelsen Method and Molecular Rapid Test

Contact Info

Product

Resources

About