Gallstone pancreatitis in children: atypical presentation and review

Akel, Samir; Khalifeh, Mohamad; Akel, Madeleine

doi:10.1007/s00431-005-1675-2

Cited by 8 publications

(1 citation statement)

References 10 publications

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“…A linear model’s percentage of variation in the response variable is shown by the R-Squared (coefficient of determination). R-Squared is a measure of how well a model matches experimental data [ 16 ]. Model fit is thought to be good when the R-Squared value is at least 0.8.…”

Section: Resultsmentioning

confidence: 99%

Characterization of a thermostable protease from Bacillus subtilis BSP strain

Majeed,

Lee,

Orts

et al. 2024

BMC Biotechnol

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This study used conservative one variable-at-a-time study and statistical surface response methods to increase the yields of an extracellular thermostable protease secreted by a newly identified thermophilic Bacillus subtilis BSP strain. Using conventional optimization techniques, physical parameters in submerged fermentation were adjusted at the shake flask level to reach 184 U/mL. These physicochemical parameters were further optimized by statistical surface response methodology using Box Behnken design, and the protease yield increased to 295 U/mL. The protease was purified and characterized biochemically. Both Ca2+ and Fe2+ increased the activity of the 36 kDa protease enzyme. Based on its strong inhibition by ethylenediaminetetracetate (EDTA), the enzyme was confirmed to be a metalloprotease. The protease was also resistant to various organic solvents (benzene, ethanol, methanol), surfactants (Triton X-100), sodium dodecyl sulfate (SDS), Tween 20, Tween-80 and oxidants hydrogen per oxide (H2O2). Characteristics, such as tolerance to high SDS and H2O2 concentrations, indicate that this protease has potential applications in the pharmaceutical and detergent industries.

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Section: Resultsmentioning

confidence: 99%