Gallic acid prevents nonsteroidal anti-inflammatory drug-induced gastropathy in rat by blocking oxidative stress and apoptosis

Tryptamine-Gallic Acid Hybrid Prevents Non-steroidal Anti-inflammatory Drug-induced Gastropathy

“…where ⌬a t T is the absorbance change after the time interval t (6 min) relative to the tested tryptamine derivatives at a concentration of 10 M, and ⌬a t Fe 2ϩ is the absorbance change at the same time interval relative to ferrous sulfate at the same concentration (4).…”

“…The fixed tissues were then dehydrated and embedded in paraffin for preparing semithin sections (4). A microtome was used to prepare the semithin sections, which were then taken over poly-L-lysine-coated glass slides for hematoxylin-eosin staining.…”

“…Gastric Mucosal Cell Culture-Gastric mucosal cells were isolated and cultured as described earlier (4). Mucosa from the rat stomach was scraped in Hanks' balanced salt solution (HBSS) (pH 7.4) containing 100 units/ml penicillin, 100 units/ml streptomycin, and 10 g/ml gentamycin.…”

“…Tetraethoxypropane was used as a standard. Protein carbonyl was measured by following the standard colorimetric method that measures the binding of dinitrophenylhydrazine to the carbonyl group and was quantified by taking the absorbance at 362 nm (4,43).…”

“…The fluorescence of each sample was measured in a Hitachi F-7000 fluorescence spectrofluorimeter (excitation 490 nm, emission 530 nm for JC-1 monomer; emission 590 nm for JC-1 aggregates). ⌬⌿ m was expressed as fluorescence ratio of 590 nm/530 nm (4,43).…”