Gallaecimonas mangrovi sp. nov., a novel bacterium isolated from mangrove sediment

Abstract

A Gram-stain negative, rod-shaped, non-motile, strictly aerobic bacterium HK-28 was isolated from a mangrove sediment sample in Haikou city, Hainan Province, China. Strain HK-28 was able to grow at 10-45 °C (optimum 25-30 °C), pH 5.0-8.5 (optimum 6.0-7.0) and 0.5-12.0% (w/v) NaCl (optimum 1.0-3.0%, w/v). The major cellular fatty acids were C, Summed Feature 8 (C ω7c and/or C ω6c), Summed Feature 3 (C ω7c and/or C ω6c), C, C 3-OH and Cω8c. Ubiquinone-8 (Q-8) was the predominant respiratory quinone. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, four unidentified phospholipids, two unidentified glycolipid, an unidentified glycophospholipid, an unidentified aminolipid and an unidentified lipid. The DNA G+C content was 50.2 mol%. Accoroding to 16S rRNA gene sequence similarities, strain HK-28 shared 97.1 and 96.7% sequence similarities to the validly named species Gallaecimonas xiamenensis MCCC 1A01354 and Gallaecimonas pentaromativorans MCCC 1A06435, respectively, and shared lower sequence similarities (< 92.0%) to all other genera. Phylogenetic analysis showed strain HK-28 was clustered with G. pentaromativorans MCCC 1A06435 and G. xiamenensis MCCC 1A01354. Strain HK-28 showed low DNA-DNA relatedness with G. xiamenensis MCCC 1A01354 (28.3 ± 1.5%) and G. pentaromativorans MCCC 1A06435 (25.2 ± 2.4%). On the basis of phenotypic, chemotaxonomic and genotypic characteristics, strain HK-28 is considered to represent a novel species in the genus Gallaecimonas, for which the name Gallaecimonas mangrovi sp. nov. is proposed. The type strain is HK-28 (= KCTC 62177 = MCCC 1K03441).

Use of modified ichip for the cultivation of thermo-tolerant microorganisms from the hot spring

“…Isolation efforts inspired by the ichip were also performed using many kinds of sediments, such as marine sediments, mangrove sediment, and estuarine sediment, etc.. These studies have also helped us obtain many unculturable microorganisms, such as Actinomycetota resources and harmful substance degrading bacteria resources [39][40][41]. Generally speaking, this platform has been shown to increase microbial recovery from 5-to 300-fold, depending on the study [19].…”

Methanolobus mangrovi sp. nov. and Methanolobus sediminis sp. nov, two novel methylotrophic methanogens isolated from mangrove sediments in China

“…Cells of strains FTZ2 T and FTZ6 T in the late-exponential phase were observed by SEM [39] and TEM [15]. Cell susceptibility to sodium dodecyl sulphate (SDS) was determined by measuring cell growth after injection of anoxic SDS stocks to cultures in mid-logarithmic phase to a final concentration of 0.01 % (w/v) [40].…”

“…Genome sequencing was performed by the Illumina NovaSeq 6000 PE150 and Oxford Nanopore PromethION 48 platforms (Magigene, Guangdong, PR China). Quality controls of Illumina and Nanopore reads were performed as previously described [15]. Then, clean reads from the Illumina and Nanopore sequencing were assembled with Unicycler (version 0.4.8) with default options [16].…”

Methods and Strategies to Uncover Coral-Associated Microbial Dark Matter

“…This antibiotic was the first new class of antimicrobials found after 30 years, representing a promising alternative chemotherapeutic agent to control infections caused by multidrug-resistant bacteria. Other studies have shown the applicability of iChip-like devices to culture microbes from aquatic environments and organisms, such as from mangroves ( 105 ) and sponges ( 37 ).…”