Galectins detection for the diagnosis of chronic diseases: An emerging biosensor approach

Capasso, Domenica; Pirone, Luciano; Gaetano, Sonia Di; Russo, Rita; Frisulli, Valeria; Antonacci, Amina; Pedone, Emilia; Scognamiglio, Viviana

doi:10.1016/j.trac.2023.116952

Cited by 2 publications

(2 citation statements)

References 82 publications

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“… 7 This methodology has been applied to several drugs and biomolecules to understand the mechanism of hybrid nanoparticles and the competitive effect between several capping agents and drugs on the growth process of gold nanoparticles. 8 , 9 , 20 , 21 These authors have applied this methodology on gemcitabine (GEM) in which the original nanoflower shapes are produced after the growth process.…”

Section: Resultsmentioning

confidence: 99%

Spectroscopic Assessment of Doxorubicin (DOX)-Gemcitabine (GEM) Gold Complex Nanovector as Diagnostic Tool of Galectin-1 Biomarker

Khan,

Cherni,

Dekhili

et al. 2024

NSA

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Introduction The aim of this study is focused on the development of theranostic hybrid nanovectors based on gold-doxorubicin (DOX)-gemcitabine (GEM) complexes and their active targeting with Galectin-1 (Gal-1) as a promising therapeutic and prognostic marker in cancer. Methods For this purpose, a gold salt (HAuCl 4 ) interacts with antitumor drugs (DOX; GEM) by chelation and then stabilizes with dicarboxylic acid-terminated polyethylene glycol (PEG) as a biocompatible surfactant. The proposed methodology is fast and reproducible, and leads to the formation of a hybrid nanovector named GEM@DOX IN PEG-AuNPs, in which the chemo-biological stability was improved. All synthetic chemical products were evaluated using various spectroscopic techniques (Raman and UV–Vis spectroscopy) and transmission electron microscopy (TEM). Results To conceive a therapeutic application, our hybrid nanovector (GEM@DOX IN PEG-AuNPs) was conjugated with the Galectin-1 protein (Gal-1) at different concentrations to predict and specifically recognize cancer cells. Gal-1 interacts with GEM@DOX in PEG-AuNPs, as shown by SPR and Raman measurements. We observed both dynamic variation in the plasmon position (SPR) and Raman band with Gal-1 concentration. Discussion We identified that GEM grafted electrostatically onto DOX IN PEG-AuNPs assumes a better chemical conformation, in which the amino group (NH 3 + ) reacts with the carboxylic (COO − ) group of PEG diacide, whereas the ciclopenthanol group at position C-5’ reacts with NH 3 + of DOX. Conclusion This study opens further way in order to built “smart nanomedical devices” that could have a dual application as therapeutic and diagnostic in the field of nanomedicine and preclinical studies associated.

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Section: Resultsmentioning

confidence: 99%

Spectroscopic Assessment of Doxorubicin (DOX)-Gemcitabine (GEM) Gold Complex Nanovector as Diagnostic Tool of Galectin-1 Biomarker

Khan,

Cherni,

Dekhili

et al. 2024

NSA

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“…Microbial lectins are involved in host colonization, whereas some animal lectins can mediate immune recognition of microbial and parasite envelope glycans and promote events like activation and regulation, mediating immunomodulation and inflammation processes. Within the lectin family, galectins represent an evolutionarily conserved group of proteins with the ability to bind β-galactosides via characteristic carbohydrate recognition domains (CRDs) [ 2 , 3 , 4 , 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Biophysical and Structural Characterization of the Interaction between Human Galectin-3 and the Lipopolysaccharide from Pseudomonas aeruginosa

Pirone,

Lenza,

Di Gaetano

et al. 2024

IJMS

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Given the significant involvement of galectins in the development of numerous diseases, the aim of the following work is to further study the interaction between galectin-3 (Gal3) and the LPS from Pseudomonas aeruginosa. This manuscript focused on the study of the interaction of the carbohydrate recognition domain of Gal3 with the LPS from Pseudomonas aeruginosa by means of different complementary methodologies, such as circular dichroism; spectrofluorimetry; dynamic and static light scattering and evaluation of the impact of Gal3 on the redox potential membranes of Escherichia coli and P. aeruginosa cells, as well as ITC and NMR studies. This thorough investigation reinforces the hypothesis of an interaction between Gal3 and LPS, unraveling the structural details and providing valuable insights into the formation of these intricate molecular complexes. Taken together, these achievements could potentially prompt the design of therapeutic drugs useful for the development of agonists and/or antagonists for LPS receptors such as galectins as adjunctive therapy for P. aeruginosa.

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Galectins detection for the diagnosis of chronic diseases: An emerging biosensor approach

Cited by 2 publications

References 82 publications

Spectroscopic Assessment of Doxorubicin (DOX)-Gemcitabine (GEM) Gold Complex Nanovector as Diagnostic Tool of Galectin-1 Biomarker

Spectroscopic Assessment of Doxorubicin (DOX)-Gemcitabine (GEM) Gold Complex Nanovector as Diagnostic Tool of Galectin-1 Biomarker

Biophysical and Structural Characterization of the Interaction between Human Galectin-3 and the Lipopolysaccharide from Pseudomonas aeruginosa

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