Galectin signature of the choriocarcinoma JAr cells: Galectin‐1 as a modulator of invasiveness in vitro

Kolundžić, Nikola; Ćujić, Danica; Rabi, Tamara Abu; Bojić‐Trbojević, Žanka; Kadoya, Toshihiko; Vićovac, Ljiljana

doi:10.1002/mrd.22515

Cited by 7 publications

(9 citation statements)

References 47 publications

(64 reference statements)

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“…Among trophoblast cells, galectin-3 was localised in villous cytotrophoblast, and the cytotrophoblast of middle and distal cell columns of anchoring villi 10 . Expression of this galectin was also reported in freshly isolated trophoblast cells, extravillous HTR-8/SVneo cells, BeWo, JAr and JEG-3 choriocarcinoma cells 10,11,23 . It has been reported that galectin-3 can be associated with some pathological conditions involving the trophoblast, such as preeclampsia, HELLP syndrome and trophoblast malignancies 24,25 .…”

Section: Introductionsupporting

confidence: 54%

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Human trophoblast requires galectin-3 for cell migration and invasion

Bojić‐Trbojević

Krivokuća

Vilotić

et al. 2019

Sci Rep

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Invasive extravillous cytotrophoblast of the human placenta expresses galectins-1, -3, and -8 in vivo and in vitro. This study aimed to investigate the potential role of galectin-3 in cell migration and invasion, using recombinant human galectin-3 (rhgalectin-3), small molecule galectin inhibitor I47, and galectin-3 silencing. HTR-8/SVneo cell migration was stimulated by rhgalectin-3 and reduced by I47, which could be neutralised by rhgalectin-3. Inhibitor specificity and selectivity for the galectins expressed in extravillous trophoblast were validated in solid phase assays using recombinant galectin-1, -3, -8, confirming selectivity for galectin-3. HTR-8/SVneo cell migration and invasion, and invasion by isolated trophoblast cells in primary culture were significantly reduced in the presence of I47, which could be restored by rhgalectin-3. Upon HTR-8/SVneo cell treatment with galectin-3 siRNA both LGALS3 and galectin-3 protein were dramatically decreased. Silencing of galectin-3 induced significant reduction in cell migration and invasion, which was restored by rhgalectin-3. The influence on known mediators of cell invasion, MMP2 and -9, and integrins α1, α5, and β1 was followed in silenced cells, showing lower levels of MMPs and a large reduction in integrin subunit β1. These results show that galectin-3 acts as a pro-invasive autocrine/paracrine factor in trophoblast in vitro.

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Section: Introductionsupporting

confidence: 54%

“…Expression of galectin-3 has been previously documented for villous cytotrophoblast, cell columns, isolated cytotrophoblast and trophoblast derived cell lines 10,11,23 . Here, the expression pattern and subcellular distribution of galectin-3 in HTR-8/SVneo cells was further examined using polyclonal anti-galectin-3 antibodies.…”

Section: Resultsmentioning

confidence: 93%

Human trophoblast requires galectin-3 for cell migration and invasion

Bojić‐Trbojević

Krivokuća

Vilotić

et al. 2019

Sci Rep

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“…The research from our lab has previously shown that galectin-1 may act as a part trophoblast invasion machinery [41]. Both oxidized and reduced forms of recombina human galectin-1 (rhgal-1) stimulated first-trimester CTB and HTR-8/SVneo cell invasio This stimulation was reversed by neutralizing antibodies, as was by treatment with inhi itory sugar lactose, suggesting a lectin-type interaction-dependent mode of stimulatio These observations were replicated for the JAR trophoblast cell line, as well [42]. Prolife ation of BeWo cells was inhibited by the treatment with galectin-1 [43].…”

Section: Galectins and Human Trophoblast Cell Functionmentioning

confidence: 64%

Galectins in Early Pregnancy and Pregnancy-Associated Pathologies

Krivokuća

Vilotić

Nacka-Aleksić

et al. 2021

IJMS

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Galectins are a family of conserved soluble proteins defined by an affinity for β-galactoside structures present on various glycoconjugates. Over the past few decades, galectins have been recognized as important factors for successful implantation and maintenance of pregnancy. An increasing number of studies have demonstrated their involvement in trophoblast cell function and placental development. In addition, several lines of evidence suggest their important roles in feto-maternal immune tolerance regulation and angiogenesis. Changed or dysregulated galectin expression is also described in pregnancy-related disorders. Although the data regarding galectins’ clinical relevance are still at an early stage, evidence suggests that some galectin family members are promising candidates for better understanding pregnancy-related pathologies, as well as predicting biomarkers. In this review, we aim to summarize current knowledge of galectins in early pregnancy as well as in pregnancy-related pathologies.

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“…The data presented show that all three analyzed gals were released from HTR-8/SVneo cells, as assessed by western blot of different fractions of CM. Our group previously demonstrated that gal-1 is secreted by HTR-8/SVneo cells (Ćujić et al, 2013) and the presence of all three galectins in CM of the choriocarcinoma cell line JAr (Kolundzic et al, 2015). Secreted gal-3 was detected in CM from another choriocarcinoma cell line, BeWo (Yang et al, 2014).…”

Section: Discussionmentioning

confidence: 86%

“…Gal-1, gal-3, and gal-8 have been detected in conditioned medium (CM) of trophoblast cell lines (Yang et al, 2011;Ćujić et al, 2013;Kolundzic et al, 2015). As gals lack a signal sequence, questions pertinent to the route of gal secretion in trophoblasts remain unresolved (Hughes, 1999).…”

Section: Introductionmentioning

confidence: 99%

Extracellular presence/release of galectins from HTR-8/SVneo extravillous trophoblast cells*

Ćujić¹,

Kosanović²,

Krivokuća³

et al. 2017

Turk J Biol

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Galectins (gals) are β-galactoside binding lectins, involved in many processes at the fetomaternal interface where they can exert their roles both in and out of cells. The aim of this work was to explore the extracellular presence/release of HTR-8/SVneo extravillous trophoblast cell line galectins. To that end, conditioned medium (CM) from HTR-8/SVneo cell culture was fractionated into a high molecular mass fraction (HMF) and low molecular mass fraction (LMF) using 100 kDa cut-off concentrators. In addition, extracellular vesicles (EVs) were isolated from CM by ultracentrifugation. Size and shape of the EVs were analyzed by transmission electron microscopy and their galectin mRNA content was determined by real-time PCR. The presence of galectins in fractions of HTR-8/SVneo CM and EVs was detected by western blot. All three galectins expressed by HTR-8/SVneo cells (gal-1, gal-3, and gal-8) were detected in the CM, HMF, and EVs, while only gal-1 was found in the LMF. In addition, EVs contained all three galectin mRNAs. These results reveal that free, complexed, and EV-associated forms of galectins were released from extravillous trophoblast cells, suggesting their potential to exert extracellular functions both in their immediate vicinity at the fetomaternal interface and distant locations.

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Galectin signature of the choriocarcinoma JAr cells: Galectin‐1 as a modulator of invasiveness in vitro

Cited by 7 publications

References 47 publications

Human trophoblast requires galectin-3 for cell migration and invasion

Human trophoblast requires galectin-3 for cell migration and invasion

Galectins in Early Pregnancy and Pregnancy-Associated Pathologies

Extracellular presence/release of galectins from HTR-8/SVneo extravillous trophoblast cells*

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