Galectin-3 regulation of wound healing and fibrotic processes: insights for chronic skin wound therapeutics

McLeod, Karrington; Walker, John T.; Hamilton, David A.

doi:10.1007/s12079-018-0453-7

Cited by 42 publications

(33 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…S7) is indicative of a role for CD4 + CLA + CD103 + T cells in promoting normal tissue homeostasis and repair in the skin (33) (34). Consistent with this, CD4 + CLA + CD103 + cells in the blood and skin differentially expressed a set of genes implicated in tissue-repair responses, such as CD9 (24), MUC16 (35) (36), and LGALS3 (37), as well as the receptor components for the damage-associated epithelial cell products IL-25 (IL17RA, IL17RB) and prostaglandin E2 (PTGER3) ( Fig. 4F, Fig.…”

Section: Cd4 + Cla + Cd103 + T Cells From Blood and Skin Share A Transupporting

confidence: 58%

Human CD4⁺CD103⁺ cutaneous resident memory T cells are found in the circulation of healthy subjects

Klicznik

Morawski²,

Höllbacher³

et al. 2018

Preprint

View full text Add to dashboard Cite

Tissue-resident memory T cells (TRM) persist locally in non-lymphoid tissues where they provide front-line defense against recurring insults. TRM at barrier surfaces express the markers CD103 and/or CD69 which function to retain them in epithelial tissues. In humans, neither the long-term migratory behavior of TRM nor their ability to re-enter the circulation and potentially migrate to distant tissue sites have been investigated. Using tissue explant cultures, we found that CD4 + CD69 + CD103 + TRM in human skin can downregulate CD69 and exit the tissue.Additionally, we identified a skin-tropic CD4 + CD69 -CD103 + population in human lymph and blood that is transcriptionally, functionally and clonally related to the CD4 + CD69 + CD103 + TRM population in the skin. Using a skin xenograft model, we confirmed that a fraction of the human cutaneous CD4 + CD103 + TRM population can re-enter circulation, and migrate to secondary human skin sites where they re-assume a TRM phenotype. Thus, our data challenge current concepts regarding the strict tissue compartmentalization of CD4 + T cell memory in humans.

show abstract

Section: Cd4 + Cla + Cd103 + T Cells From Blood and Skin Share A Transupporting

confidence: 58%

Human CD4⁺CD103⁺ cutaneous resident memory T cells are found in the circulation of healthy subjects

Klicznik

Morawski²,

Höllbacher³

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…Furthermore, it is also expressed by other migrating cells into the skin, including dendritic cells, fibroblasts, and monocytes [122]. Deletion of Gal-3 in mouse models shows different inflammatory or remodeling effects on skin compared to other organs such as heart, lung and kidney [123]. For example, in psoriasis patients, epidermal Gal-3 expression is significantly decreased in the psoriatic skin lesions, but not in non-psoriatic skin lesions.…”

Section: Skin Diseases and Its Repairmentioning

confidence: 99%

Galectin-3 as a Next-Generation Biomarker for Detecting Early Stage of Various Diseases

et al. 2020

View full text Add to dashboard Cite

Galectin-3 is a β-galactoside-binding lectin which is important in numerous biological activities in various organs, including cell proliferation, apoptotic regulation, inflammation, fibrosis, and host defense. Galectin-3 is predominantly located in the cytoplasm and expressed on the cell surface, and then often secreted into biological fluids, like serum and urine. It is also released from injured cells and inflammatory cells under various pathological conditions. Many studies have revealed that galectin-3 plays an important role as a diagnostic or prognostic biomarker for certain types of heart disease, kidney disease, viral infection, autoimmune disease, neurodegenerative disorders, and tumor formation. In particular, it has been recognized that galectin-3 is extremely useful for detecting many of these diseases in their early stages. The purpose of this article is to review and summarize the recent literature focusing on the biomarker characteristics and long-term outcome predictions of galectin-3, in not only patients with various types of diseases, but associated animal models.

show abstract

“…These results are in agreement with similar studies on other ligand systems targeting lectins involved in cell migration. [ 77,86–89 ] For example, Dion and co‐workers observed a delay in wound healing of around 20% for the treatment of keratinocytes with lactosamine‐based (2‐naphthyl)methyl compounds inspired by TD139, which is currently in clinical trials. [ 57,88,89 ] Raman and co‐workers achieved a delay of 20–30% through the incubation of MCF 7 cells with a nucleoside analog addressing RNA helicase.…”

Section: Resultsmentioning

confidence: 99%

Sequence‐Defined Heteromultivalent Precision Glycomacromolecules Bearing Sulfonated/Sulfated Nonglycosidic Moieties Preferentially Bind Galectin‐3 and Delay Wound Healing of a Galectin‐3 Positive Tumor Cell Line in an In Vitro Wound Scratch Assay

Freichel

Heine

Laaf

et al. 2020

Macromolecular Bioscience

View full text Add to dashboard Cite

Within this work, a new class of sequence‐defined heteromultivalent glycomacromolecules bearing lactose residues and nonglycosidic motifs for probing glycoconjugate recognition in carbohydrate recognition domain (CRD) of galectin‐3 is presented. Galectins, a family of β‐galactoside‐binding proteins, are known to play crucial roles in different signaling pathways involved in tumor biology. Thus, research has focused on the design and synthesis of galectin‐targeting ligands for use as diagnostic markers or potential therapeutics. Heteromultivalent precision glycomacromolecules have the potential to serve as ligands for galectins. In this work, multivalency and the introduction of nonglycosidic motifs bearing either neutral, amine, or sulfonated/sulfated groups are used to better understand binding in the galectin‐3 CRD. Enzyme‐linked immunosorbent assays and surface plasmon resonance studies are performed, revealing a positive impact of the sulfonated/sulfated nonglycosidic motifs on galectin‐3 binding but not on galectin‐1 binding. Selected compounds are then tested with galectin‐3 positive MCF 7 breast cancer cells using an in vitro would scratch assay. Preliminary results demonstrate a differential biological effect on MCF 7 cells with high galectin‐3 expression in comparison to an HEK 293 control with low galectin‐3 expression, indicating the potential for sulfonated/sulfated heteromultivalent glycomacromolecules to serve as preferential ligands for galectin‐3 targeting.

show abstract

Galectin-3 regulation of wound healing and fibrotic processes: insights for chronic skin wound therapeutics

Cited by 42 publications

References 59 publications

Human CD4⁺CD103⁺ cutaneous resident memory T cells are found in the circulation of healthy subjects

Human CD4⁺CD103⁺ cutaneous resident memory T cells are found in the circulation of healthy subjects

Galectin-3 as a Next-Generation Biomarker for Detecting Early Stage of Various Diseases

Sequence‐Defined Heteromultivalent Precision Glycomacromolecules Bearing Sulfonated/Sulfated Nonglycosidic Moieties Preferentially Bind Galectin‐3 and Delay Wound Healing of a Galectin‐3 Positive Tumor Cell Line in an In Vitro Wound Scratch Assay

Contact Info

Product

Resources

About

Galectin-3 regulation of wound healing and fibrotic processes: insights for chronic skin wound therapeutics

Cited by 42 publications

References 59 publications

Human CD4+CD103+ cutaneous resident memory T cells are found in the circulation of healthy subjects

Human CD4+CD103+ cutaneous resident memory T cells are found in the circulation of healthy subjects

Galectin-3 as a Next-Generation Biomarker for Detecting Early Stage of Various Diseases

Sequence‐Defined Heteromultivalent Precision Glycomacromolecules Bearing Sulfonated/Sulfated Nonglycosidic Moieties Preferentially Bind Galectin‐3 and Delay Wound Healing of a Galectin‐3 Positive Tumor Cell Line in an In Vitro Wound Scratch Assay

Contact Info

Product

Resources

About

Human CD4⁺CD103⁺ cutaneous resident memory T cells are found in the circulation of healthy subjects

Human CD4⁺CD103⁺ cutaneous resident memory T cells are found in the circulation of healthy subjects