Galectin-3 Plays an Important Pro-inflammatory Role in the Induction Phase of Acute Colitis by Promoting Activation of NLRP3 Inflammasome and Production of IL-1β in Macrophages

Marković, Bojana Simović; Nikolić, Aleksandar; Gazdic, Marina; Bojić, Sanja; Vučićević, Ljubica; Kosic, Milica; Mitrović, Slobodanka; Milosavljević, Mirjana; Besra, Gurdyal S.; Trajkovič, Vladimir; Arsenijević, Nebojša; Lukic, Miodrag L.; Volarević, Vladislav

doi:10.1093/ecco-jcc/jjw013

Cited by 102 publications

(68 citation statements)

References 44 publications

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“…Human Gal‐3 is a chimera‐type β‐galactoside‐binding galectin protein that exerts physiological effects through carbohydrate‐recognition domains and is mainly secreted by cardiac myocytes and inflammatory cells . As Gal‐3 level had been reported increases with reducing eGFR independent of cardiovascular complications (for example heart failure), and also elevated in patients with activated inflammation . CRP and BMI has also been reported associated with Gal‐3 level, however we did not found the correlation in our cohort.…”

Section: Discussioncontrasting

confidence: 60%

The predictive value of serum galectin 3 for abdominal aortic calcification in maintenance hemodialysis patients: A prospective cohort study

Wang

Chen

et al. 2020

Hemodialysis International

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Introduction: Heterotopic vascular calcification is a common complication of maintenance hemodialysis (MHD) patients. Galectin 3 (Gal-3) has been reported to be associated with cardiovascular calcification. The current study aims to explore the potential predictive value of serum Gal-3 for severe abdominal aortic calcification (AAC) and AAC progression in MHD patients.Methods: A prospective cohort who underwent hemodialysis during July 2014 at the Blood Purification Center of Ruijin Hospital were followed up for 3 years. Two AAC assessments were performed: one at baseline and one after the 3-year follow-up period. Serum Gal-3 was detected with quantitative ELISA kits. SPSS 23.0 and MedCalc 11.4.2.0 were used to analyze the data.Findings: One hundred and fifty-two patients were recruited. Approximately 59.9% were male, the median age was 60 (50-67) years. Logistic regression analysis indicated that serum Gal-3 was an independent risk factor for both follow-up severe AAC and AAC progression. Receiver operating characteristic (ROC) curve analysis revealed significant prognostic value of serum Gal-3 for predicting severe AAC and AAC progression within 3 years. Discussion:We found serum Gal-3 is correlated to vascular calcification in ESRD patients. Gal-3 may be a potential biomarker of vascular calcification for MHD patients.

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Section: Discussioncontrasting

confidence: 60%

The predictive value of serum galectin 3 for abdominal aortic calcification in maintenance hemodialysis patients: A prospective cohort study

Wang

Chen

et al. 2020

Hemodialysis International

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“…Although further studies with larger sample size are needed to confirm this conclusion, it is possible that the increase in serum galectins might represent an early event in the inflammatory cascade and not subsequently rise further with increase of disease activity. The recent reports showing that several galectin members play key pathogenic roles in animal models of colitis [27] and galectin-3 caused macrophage activation in the induction phase of colitis in a dextran sodium sulphate animal model of colitis [28] are in line with this possibility. The increased level of serum galectin-3 in IBD patients found in this study is in agreement with an early report showing higher serum galectin-3 level in IBD patients, irrespective of their disease activity, compared with healthy people [22].…”

Section: Discussionmentioning

confidence: 65%

Serum galectins as potential biomarkers of inflammatory bowel diseases

Subramanian

2020

PLoS ONE

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The inflammatory bowel diseases (IBD), which include mainly Crohn's disease (CD) and ulcerative colitis (UC), are common chronic inflammatory conditions of the digestive system. The diagnosis of IBD relies on the use of a combination of factors including symptoms, endoscopy and levels of serum proteins such as C-reactive protein (CRP) or faecal calprotectin. Currently there is no single reliable biomarker to determine IBD. Galectins are a family of galactoside-binding proteins that are commonly altered in the circulation of disease conditions such as cancer and inflammation. This study investigated serum galectin levels as possible biomarkers in determining IBD and IBD disease activity. Levels of galectins-1, -2, -3, -4, -7 and -8 were analysed in 208 samples from ambulant IBD patients (97 CD, 71 UC) patients and 40 from healthy people. Disease activity was assessed using Harvey-Bradshaw Index for CD and simple clinical colitis activity index for UC. The relationship of each galectin in determining IBD and IBD disease activity were analysed and compared with current IBD biomarker CRP. It was found that serum level of galectin-1 and -3, but not galectins-2, -4, -7 and -8, were significantly higher in IBD patients than in healthy people. At cut-off of 4.1ng/ml, galectin-1 differentiated IBD from healthy controls with 71% sensitivity and 87% specificity. At cut-off of 38.5ng/ml, galectin-3 separated IBD from healthy controls with 53% sensitivity and 87% specificity. None of the galectins however were able to distinguish active disease from remission in UC or CD. Thus, levels of galectins-1 and -3 are significantly elevated in both UC and CD patients compared to healthy people. Although the increased galectin levels are not able to separate active and inactive UC and CD, they may have the potential to be developed as useful biomarkers for IBD diagnosis either alone or in combination with other biomarkers.

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“…It plays key roles in physiological and pathological processes such as inflammation and fibrosis, which are involved in the development of pulmonary arterial remodeling and stiffness. Gal‐3 is expressed in fibroblasts, endothelial cells, and inflammatory cells such as macrophages, which are associated with cardiac dysfunction through the induction of cardiac fibroblast proliferation, collagen deposition, and ventricular dysfunction . Gal‐3 also promotes vascular damage and cardiovascular fibrosis …”

Section: Introductionmentioning

confidence: 99%

Galectin‐3 contributes to vascular fibrosis in monocrotaline‐induced pulmonary arterial hypertension rat model

Wang

Zhang

et al. 2016

J Biochem & Molecular Tox

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Galectin-3 (Gal-3) plays a critical role in vascular inflammation and fibrosis. The role of TGF-β1 in mediating pulmonary vascular fibrosis is well documented; thus, we suspected that Gal-3 could be an important factor in TGF-β1-induced fibrosis in pulmonary adventitial fibroblasts (PAFs). We treated rats with monocrotaline (MCT) and cultured PAFs with TGF-β1 to stimulate fibrosis. We found that MCT injection induced vessel thickening and extracellular matrix deposition in vivo. TGF-β1 stimulated the production of collagen and fibronectin (Fn) protein in vitro. TGF-β1 promoted the expression of Gal-3 and its translocation, while silencing Gal-3 reduced Col-1a deposition. Blockage of STAT3 decreased the expression of Gal-3 induced by TGF-β1. Gal-3 increased Col-1a accumulation and downregulated matrix metallopeptidase 9 (MMP-9) expression in PAFs, but it did not affect Fn expression. These findings demonstrate that Gal-3 is required for TGF-β1-stimulated vascular fibrosis via a STAT3 signaling cascade and that MMP-9 is also involved in TGF-β1/Gal-3-induced vascular fibrosis.

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Galectin-3 Plays an Important Pro-inflammatory Role in the Induction Phase of Acute Colitis by Promoting Activation of NLRP3 Inflammasome and Production of IL-1β in Macrophages

Abstract: Gal-3 expression promotes acute DSS-induced colitis and plays an important pro-inflammatory role in the induction phase of colitis by promoting the activation of NLRP3 inflammasome and production of IL-1β in macrophages.

Cited by 102 publications

References 44 publications

The predictive value of serum galectin 3 for abdominal aortic calcification in maintenance hemodialysis patients: A prospective cohort study

The predictive value of serum galectin 3 for abdominal aortic calcification in maintenance hemodialysis patients: A prospective cohort study

Serum galectins as potential biomarkers of inflammatory bowel diseases

Galectin‐3 contributes to vascular fibrosis in monocrotaline‐induced pulmonary arterial hypertension rat model

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