Galectin-10 Is Released in the Nasal Lavage Fluid of Patients with Aspirin-Sensitive Respiratory Disease

Abstract

<jats:p>The aim of this work was to determine the presence of galectin-10 in nasal lavage fluid (NLF) of patients with aspirin-sensitive respiratory disease (ASRD) before and after challenge with L-ASA (aspirin) by ELISA. Fifteen ASRD patients, ten aspirin-tolerant asthmatics (ATA), and fifteen healthy controls (HC) were studied. The baseline presence of Galectin-10 in PBMC was determined using real time PCR. Galectin-10 was evaluated in tissue of nasal polyps by western blot. Our results showed a lower expression in PBMC of ASRD patients than in ATA and healthy controls. However, a higher concentration of galectin-10 in NLF was found in ASRD patients before and after L-ASA challenge; western blot confirmed a high expression of galectin-10 in tissue from nasal polyps obtained from ASRD patients. Our results suggest a probable role of galectin-10 in the inflammatory response observed in ASRD patients; however, confirmatory studies are needed.</jats:p>

Barrier function of the nasal mucosa in health and type‐2 biased airway diseases

“…Galectin‐3 is furthermore reported to be upregulated in the nasal lavage fluid of patients with symptomatic birch and/or grass pollen induced intermittent allergic rhinitis versus healthy volunteers . The latter was confirmed in a mouse model of allergic rhinitis . Also, galectin‐10 is reported to be increased in nasal lavage fluid and nasal polyp tissue of patients with aspirin‐sensitive respiratory disease .…”

“…Galectins ‐ Galectin‐3 ↑ in nasal lavage fluid of patients with symptomatic birch and/or grass pollen induced intermittent allergic rhinitis and in a mouse model of allergic rhinitis . ‐ Galectin‐1 ↑ in CRSwNP tissue ‐ galectin‐3 ↑ in CRSwNP tissue ‐ galectin‐10 ↑ increased in nasal lavage fluid and tissue of CRSwNP (explicitly pronounced in CRSwNP patients with comorbid aspirin‐sensitive respiratory disease) …”

Towards molecular mechanisms regulating the expression of galectins in cancer cells under microenvironmental stress conditions

“…The upregulation of galectin-10 has been reported in several models of physiological and pathological stresses. Bronchial and nasal inflammation is accompanied by the activation and recruitment of eosinophils, and a corresponding accumulation of galectin-10 in peripheral blood [72], samples of sputum [73], and nasal lavage [74]. Elevated levels of galectin-10 have also been reported in gut biopsies of patients with celiac disease, an autoimmune disorder of the intestine caused by an allergy to gluten [75].…”

Nasal protein profiles in work‐related asthma caused by different exposures

“…LEG10 (aka Charcot‐Leyden crystal (CLC) protein, eosinophil lysophospholipase) has been associated with eosinophilic inflammation in allergic diseases and with the function of regulatory T cells . LEG10 is mainly released from eosinophil granules, but it is also expressed by basophils and some T cells .…”