Galectin-1 ameliorates lipopolysaccharide-induced acute lung injury via AMPK-Nrf2 pathway in mice

Huang, Xiaoting; Liu, Wei; Zhou, Yong; Sun, Mei; Yang, Huihui; Zhang, Chenyu; Tang, Siyuan

doi:10.1016/j.freeradbiomed.2019.11.011

Cited by 89 publications

(116 citation statements)

References 45 publications

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“…Thus, our findings indicate that SP has potent antioxidant properties that are likely to be responsible for its protective effect against acute lung damage that is caused by LPS treatment. Nrf2 plays an essential role in many inflammatory-and oxidative stress-related diseases, such as chronic obstructive pulmonary disease (COPD) and ALI [14,18]. Nrf2 protects a variety of tissue and cells against ROS through ARE-mediated induction of diverse antioxidant enzymes, including HO-1 and NQO1 [54].…”

Section: Discussionmentioning

confidence: 99%

“…The activation of NF-κB and MAPK pathways collaborates to induce the release of various pro-inflammatory cytokines or chemokines, including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 [12,13]. Oxidative stress also plays a major role in the pathogenesis of lung injury in LPS-induced ALI [14,15]. Nuclear factor erythroid-2 related factor 2 (Nrf2) is a redox-sensitive transcription factor and it regulates the antioxidant and detoxifying enzymes [16].…”

Section: Introductionmentioning

confidence: 99%

“…Upon activation, Nrf2 dissociates from Keap1 and translocates into the nucleus, where it binds to antioxidant response elements (AREs) in the promoter region of cytoprotective antioxidant enzymes, such as NAD(P)H quinone dehydrogenase 1 (NQO1) and heme oxygenase-1 (HO-1) [17]. Emerging evidence indicates that Nrf2 activation plays a protective role against LPS-induced oxidative stress and inflammation in the LPS-induced ALI model [14,18]. Recently, many researchers have begun to investigate the effects of herbal-derived natural products with anti-oxidant or anti-inflammatory properties on ALI [7,19].…”

Section: Introductionmentioning

confidence: 99%

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Spiraea prunifolia var. simpliciflora Attenuates Oxidative Stress and Inflammatory Responses in a Murine Model of Lipopolysaccharide-Induced Acute Lung Injury and TNF-α-Stimulated NCI-H292 Cells

Lee

Shin

et al. 2020

Antioxidants

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Spiraea prunifolia var. simpliciflora (SP) is traditionally used as an herbal remedy to treat fever, malaria, and emesis. This study aimed to evaluate the anti-oxidative and anti-inflammatory properties of the methanol extract of SP leaves in tumor necrosis factor (TNF)-α-stimulated NCI-H292 cells and in a lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. SP decreased the number of inflammatory cells and the levels of TNF-α, interleukin (IL)-1β, and IL-6 in the bronchoalveolar lavage fluid, and inflammatory cell infiltration in the lung tissues of SP-treated mice. In addition, SP significantly suppressed the mRNA and protein levels of TNF-α, IL-1β, and IL-6 in TNF-α-stimulated NCI-H292 cells. SP significantly suppressed the phosphorylation of the mitogen-activated protein kinases (MAPKs) and p65-nuclear factor-kappa B (NF-κB) in LPS-induced ALI mice and TNF-α-stimulated NCI-H292 cells. SP treatment enhanced the nuclear translocation of nuclear factor erythroid 2-related factor (Nrf2) with upregulated antioxidant enzymes and suppressed reactive oxygen species (ROS)-mediated oxidative stress in the lung tissues of LPS-induced ALI model and TNF-α-stimulated NCI-H292 cells. Collectively, SP effectively inhibited airway inflammation and ROS-mediated oxidative stress, which was closely related to its ability to induce activation of Nrf2 and inhibit the phosphorylation of MAPKs and NF-κB. These findings suggest that SP has therapeutic potential for the treatment of ALI.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Spiraea prunifolia var. simpliciflora Attenuates Oxidative Stress and Inflammatory Responses in a Murine Model of Lipopolysaccharide-Induced Acute Lung Injury and TNF-α-Stimulated NCI-H292 Cells

Lee

Shin

et al. 2020

Antioxidants

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“…These findings were consistent with the reduced RANTES, IL-6 and IL-1β we found and strongly support our results. Currently, there is increasing evidence suggesting that the infiltration of macrophages (Johnston et al, 2012;Huang et al, 2020) and neutrophils (Camp and Jonsson, 2017) are key factors in the pathogenesis of IAV-induced ALI and ARDS, especially at the early stage of disease (Huang et al, 2018), and is accompanied by an increase in the immune-pathological injury of IAV infection (Camp and Jonsson, 2017;Huang et al, 2020). Thus, the reduction of inflammatory cytokines, chemokines and cell numbers after Gal-1 treatment might have a positive effect on H1N1pdm09-induced ALI.…”

Section: Discussionmentioning

confidence: 99%

Galectin-1 Ameliorates Influenza A H1N1pdm09 Virus-Induced Acute Lung Injury

et al. 2020

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Influenza remains one of the major epidemic diseases worldwide. Acute lung injury mainly caused by excessive pro-inflammatory host immune responses leads to high mortality rates in severe influenza patients. Galectin-1, an animal lectin ubiquitously expressed in mammalian tissues, is reported to play important roles in viral diseases. Here, we established murine and A549 cell models to explore the potential roles of galectin-1 treatment in H1N1pdm09-induced acute lung injury. We found that galectin-1 protein level was elevated in A549 cell culture supernatants and mouse BALF after H1N1pdm09 challenge. In vivo experiments showed recombinant galectin-1 treatment reduced wet/dry weight ratio, inflammatory cell infiltration in mouse lungs and mediated the expression of cytokines and chemokines including IL-1β, IL-6, IL-10, IL-12(p40), IL-12(p70), G-CSF, MCP-1, MIP-1α and RANTES in serum and BALF of infected mice. Reduced apoptosis and viral titers in mouse lungs were also found after galectin-1 treatment. As expected, galectin-1 treated mice performed reduced body weight loss and enhanced survival rate against H1N1pdm09 challenge. In addition, in vitro experiments showed that viral titers decreased in a dose-dependent manner and cell apoptosis in A549 cells reduced after recombinant galectin-1 treatment. Taken together, our findings indicate a potentially positive effect of Gal-1 treatment on ameliorating the progress of H1N1pdm09-induced acute lung injury and recombinant galectin-1 might serve as a new agent in treating influenza.

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“…Taken together, these results provide a potential strategy for the regulation of CYP1A1 expression in ALI. Excessive pro-inflammatory mediator production is a significant cause of ALI tissue damage [21][22][23][24]. Thus, the aim of this study was to identify a novel target for treatment of ALI, a target that would reduce a pro-inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

Knockout of cytochrome P450 1A1 enhances lipopolysaccharide‐induced acute lung injury in mice by targeting NF‐κB activation

Tian

Tang

et al. 2020

FEBS Open Bio

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Galectin-1 ameliorates lipopolysaccharide-induced acute lung injury via AMPK-Nrf2 pathway in mice

Cited by 89 publications

References 45 publications

Spiraea prunifolia var. simpliciflora Attenuates Oxidative Stress and Inflammatory Responses in a Murine Model of Lipopolysaccharide-Induced Acute Lung Injury and TNF-α-Stimulated NCI-H292 Cells

Spiraea prunifolia var. simpliciflora Attenuates Oxidative Stress and Inflammatory Responses in a Murine Model of Lipopolysaccharide-Induced Acute Lung Injury and TNF-α-Stimulated NCI-H292 Cells

Galectin-1 Ameliorates Influenza A H1N1pdm09 Virus-Induced Acute Lung Injury

Knockout of cytochrome P450 1A1 enhances lipopolysaccharide‐induced acute lung injury in mice by targeting NF‐κB activation

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