Galbanic Acid from Ferula szowitsiana Enhanced the Antibacterial Activity of Penicillin G and Cephalexin against Staphylococcus aureus

Abstract

H-, 13C-NMR) of this compound revealed that this compound was 7-[6-(b b-carboxyethyl)-5-isopropylidene-1,2-dimethylcyclo-hexylmethoxy]coumarin (galbanic acid), previously isolated from Ferula assa-foetida. In the presence of sub-inhibitory concentration of galbanic acid (100 m mg/ml) the MIC of penicillin G for S. aureus decreased from 64 to 1 (a sixteen four-fold decrease) and for cephalexin from 128 to 1 m mg/ml (a one hundred twenty eightfold decrease). The highest fold decrease in MIC was observed for cephalexin in combination of galbanic acid against test strain. These results signify that the low concentration of galbanic acid (100 m mg/ml) potentiates the antimicrobial action of penicillin G and cephalexin suggesting a possible utilization of these compounds in combination therapy against S. aureus.

Novel thermosensitive poly (N-isopropylacrylamide-co-vinylpyrrolidone-co-methacrylic acid) nanosystems for delivery of natural products.

“…The fractions were compared by TLC (silica gel using petroleum ether-EtOAc as solvent), and those giving similar coumarin spots were combined and further purified on preparative TLC to give Farnesiferol C (5.43 mg). The melting point, H-and C-NMR data of the obtained isolated farnesifeol C were confirmed according to previously published works [21,22,23].…”

Synthesis, biosynthesis and biological activities of galbanic acid – A review

“…They carried out disk diffusion and broth dilution methods to determine the antibacterial activity of the mentioned antibiotics. Their results showed that in subinhibitory concentration of GA (100 mg/mL), the MIC value of penicillin G for S. aureus was reduced from 64 mg/mL in the absence of GA to 1 mg/mL in the presence of GA, while that of cephalexin decreased from 128 mg/mL in the absence of GA to 1 mg/mL in the presence of GA. Their results suggested that GA may act as an effective adjuvant in the chemotherapy of infectious diseases (Shahverdi et al, 2007). Later on in 2009, Fazly-Bazzaz et al announced the enhancing effects of GA on isolates of S. aureus, which were clinically resistant to methicillin, tetracycline and ciprofloxacin.…”

Evaluation of anti-helmintic activity of Ferula foetida “Hing- A natural Indian spice” aqueous extract

“…Conclusions: It was concluded from the present study that the plant revealed significant anthelmintic activity. major chemical constituent called as "Galbanic acid" which is found to have high antimicrobial and antibiotic properties [4] . Sesqui-terpenoids are another group of constituents which are found to have anti-helmintic properties and are found in this plant [5] .…”