Galanthamine Production by Leucojum aestivum Cultures In Vitro

Stanilova, Marina; Molle, E.; Yanev, S.

doi:10.1016/s1099-4831(10)06805-7

Cited by 16 publications

(8 citation statements)

References 44 publications

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“…The low Amaryllidaceae alkaloid accumulation commonly observed in in vitro cultures of Leucojum aestivum [6,[9][10][11][12][13] was probably due to the fact that the level of the alkaloid biosynthesis is strongly influenced by cellular differentiation [10,[14][15][16]. In particular, enhanced synthesis of galanthamine accompanied development of somatic embryos [17].…”

Section: Introductionmentioning

confidence: 99%

Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus

et al. 2013

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In vitro cultures of Leucojum aestivum are considered as an alternative for the production of galanthamine, which is used for the symptomatic treatment of Alzheimer’s disease. We studied the effects of auxins 2,4-dichlorophenoxyacetic acid (2,4-D), 4-amino-3,5,6-trichloropicolinic acid (picloram), 3,6-dichloro-o-anisic acid (dicamba) at concentrations of 25 and 50 µM on the induction of embryogenic callus and its capacity to induce somatic embryogenesis and alkaloid accumulation. The embryogenic response of the explants was from 30% for 25 µM of dicamba to 100% for picloram (for both 25 and 50 µM). 2,4-D (50 µM) stimulated greater callus proliferation and somatic embryo induction as compared to the other auxins. Polyethylene glycol (PEG) stimulated somatic embryo maturation. Callus grown on media containing 50 µM of auxins produced fewer phenolic compounds as compared with callus grown on media containing 25 µM of auxins. GC-MS analyses showed seven alkaloids in the in vivo bulbs and two to four in callus culture. Galanthamine was detected in callus cultivated with 2,4-D (25, 50 µM), picloram (25 µM), and dicamba (50 µM). Other alkaloids, trisphaeridine, tazettine, and 11-hydroxyvittatine were accumulated only in callus growing on medium with picloram (50 µM).

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Section: Introductionmentioning

confidence: 99%

Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus

et al. 2013

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“…Among the biological factors, let's focus on the importance of cell differentiation [23,24]. According to Stanilova et al [25], galanthamine content in L. aestivum directly regenerated bulblets was 10 times greater than in L. aestivum callus-derived shoots (over 1 mg/g DW vs. 0.1 to 0.2 mg/g DW). According to the same team, genotype is another crucially important factor for the biosynthetic capacity of in vitro cultures [26].…”

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confidence: 99%

Stimulating effect of both 4’‐O‐methylnorbelladine feeding and temporary immersion conditions on galanthamine and lycorine production by Leucojum aestivum L. bulblets

Saliba

Ptak

Boisbrun

et al. 2016

Engineering in Life Sciences

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Bulb cultures of Leucojum aestivum and L. aestivum 'Gravety Giant' were subcultured in medium containing the precursor 4'-O-methylnorbelladine (MN) at various concentrations [0 (control), 0.15 and 0.3 g/L]. The cultures were conducted in bioreactor RITA R and lasted for 15, 30, 40 and 50 days. The growth rate and the alkaloid accumulation in bulblets were studied. For this latter purpose, a purification method was developed. It comprised a highly selective solid phase extraction using on the one hand, UPTI-CLEAN SI and SCX cartridges for plant extracts and on the other hand, 2H cartridges for culture media. Pure alkaloidal fractions were, thus, analyzed by LC-ESI-MS allowing the quantitative evaluation of galanthamine and lycorine from culture extracts. Precursor feeding along with temporary immersion conditions was found to significantly improve the accumulation of both galanthamine and lycorine. The maximal concentrations of galanthamine (0.81 mg/g DW) and lycorine (0.54 mg/g DW) in L. aestivum bulblets were reached, respectively, after 40 days of culture with 0.15 g/L of precursor and after 30 days of culture with 0.3 g/L of precursor. In L. aestivum 'Gravety Giant' bulb cultures, 0.3 g/L of precursor was the best condition for both galanthamine (0.6 mg/g DW after 50 days) and lycorine (1.13 mg/g DW after 30 days).

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“…Combining the technologies of DNA barcoding and metabolomics has the potential to dramatically transform the fundamental definition of a plant as a traditional medicine or dietary supplement and assist in assuring safety, efficacy, and product consistency for patients. − However, the situation is made more complex by the observation that individual clones of plants may display significant differences in concentration of a major medicinal metabolite, such as galanthamine, during a yearly growth cycle, even under controlled culture conditions . The same could be true for potential toxic metabolites.…”

Section: The Shift To Improve Traditional Medicine Quality Controlmentioning

confidence: 99%

Natural Products and Traditional Medicine: Turning on a Paradigm

2012

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Paradigm shifts in the strategies and the sciences that would enhance the quality, safety, and efficacy of traditional medicines and dietary supplements in global health care are discussed. Some of the challenges facing traditional medicine in health care are described, and the importance of defining clear goals and directions for the information systems, botany, chemistry, and biology related to plants and health care, including for drug discovery and quality control, is indicated.

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Galanthamine Production by Leucojum aestivum Cultures In Vitro

Cited by 16 publications

References 44 publications

Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus

Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus

Stimulating effect of both 4’‐O‐methylnorbelladine feeding and temporary immersion conditions on galanthamine and lycorine production by Leucojum aestivum L. bulblets

Natural Products and Traditional Medicine: Turning on a Paradigm

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