Galantamine in rheumatoid arthritis: A cross talk of parasympathetic and sympathetic system regulates synovium-derived microRNAs and related pathogenic pathways

Gowayed, Mennatallah A.; Mahmoud, Shimaa A.; Michel, Trez N.; Kamel, Maher A.; El-Tahan, Rasha A.

doi:10.1016/j.ejphar.2020.173315

Cited by 7 publications

(3 citation statements)

References 62 publications

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“…VEGF-A secretion, which reached high levels in rats with adjuvant arthritis, was significantly reduced after galantamine treatment. Galantamine has been reported to have an angiogenic and proliferative effect in its anti-arthritic role (37). In our study, we tested the angiogenic effect of galantamine in MCF-7 breast cancer cells.…”

Section: Discussionmentioning

confidence: 98%

Investigation of Antiangiogenic and Apoptotic Effects of Galantamine Obtained From Leucojum Aestivum on McF-7 Breast Cancer Cell Line

İnce,

Karabağ,

Bulduk

2023

Kocatepe Tıp Dergisi

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OBJECTIVE: The current study aims to investigate the antiangiogenic and apoptotic effects of galantamine in breast cancer cells and to add new information to the literature. MATERIAL AND METHODS: In this study, MCF-7 breast cancer cell line was used and galantamine was obtained from Leucojum aestivum by HPLC method. The effect of galantamine on cell viability was determined by CCK-8 assay at 9 different doses (control, 10, 40, 70, 100, 130, 160, 190, 210 μg/ml) including the control group. Then, to understand the effect of galantamine, other assays (total antioxidant status (TAS), total oxidant status (TOS), poly-ADP ribose polymerase (PARP), and vascular endothelial growth factor (VEGF) levels) were examined in 3 groups; control, LD50 (100 μg/ml) and high dose (210 μg/ml) group. The oxidative stress index (OSI) was then calculated. RESULTS: Galantamine decreased cell viability. The LD50 dose was determined as 100 μg/ml. There was no significant change in VEGF levels. There was a significant dose-dependent decrease in PARP levels (Control: 2.78667±0.155392, LD50: 1.51000±0.107145, High dose: 1.01000±0.054772 ng/L, p=0.000). Compared to the control group, there was a significant dose-related decrease in TAS data (Control: 0.09633±0.002658, LD50: 0.06283±0.002317, High dose: 0.04050±0.001871, p=0.000) and a significant dose-related increase in TOS data (Control: 0.12500±0.010488, LD50: 0.21667±0.015055, High dose: 0.31833±0.021370, p=0.000). A significant dose-related increase in OSI data was found. (Control: 129.964±13.018, LD50: 345.161±26.480, High dose: 788.485±78.575, p=0.000). CONCLUSIONS: It was determined that galantamine had no significant effect on angiogenesis at any dose based on VEGF levels. Galantamine had a negative effect on cell viability and proliferation at certain doses to MCF-7 cells and was found to increase oxidative stress. The decrease in PARP levels indicates the possibility that cells may result in an apoptotic process. These findings may be useful to take a different approach to the use of galantamine in cancer research.

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Section: Discussionmentioning

confidence: 98%

Investigation of Antiangiogenic and Apoptotic Effects of Galantamine Obtained From Leucojum Aestivum on McF-7 Breast Cancer Cell Line

İnce,

Karabağ,

Bulduk

2023

Kocatepe Tıp Dergisi

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“…Antidiabetic [125] , Amaryllidaceae family members Antioxidant [126] , such as Narcissus (daffodil), Anti-inflammatory [127] , Leucojum aestivum (snowflake), Anti-athritic [128] . Lycoris radiata (red spider lily) 13 Kaempferol Pteridophyta, pinophyte Anti-oxidant [129] , and Angiospermae.…”

Section: Retigabinementioning

confidence: 99%

Neuroprotective Action of Polyphenols and Phenolic Compounds: An Overview

J. Khairnar,

B. Jadhav

2023

Biosci., Biotech. Res. Asia

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A central or peripheral somatosensory nervous system lesion or illness is a common cause of neuropathic pain. In this study, we address the most recent information on neuropathy, as well as the causes, symptoms, and treatments of neurodegenerative illnesses like Alzheimer's, Parkinson's, Huntington's, and Amyotrophic Lateral Sclerosis. While, in recent years, phenolic acid supplementation has been associated to enhanced cognitive function and the prevention of cognitive deterioration. The pharmacological effects of phenolic acid are discussed in this review. And gives the overview of role of Reactive oxygen Species (ROS), oxidative stress and antioxidants in neuropathy, and stated the strong relation between stress, tension, hectic lifestyle and neurodegenerative diseases.

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“…It was proved that AChE action increases with RA and could be involved in testicular impairment through inducing inflammatory responses (Ofek et al 2007 ). Inhibitors of ACHE (ACHEIs) were proven to exert potential anti-rheumatic effect (Gowayed et al 2020 ; Kandil et al 2020 ) and could have a role in the management of testicular impairment secondary to RA providing single treatment for RA patients suffering from testicular impairment.…”

Section: Introductionmentioning

confidence: 99%

Galantamine mitigates testicular injury and disturbed spermatogenesis in adjuvant arthritic rats via modulating apoptosis, inflammatory signals, and IL-6/JAK/STAT3/SOCS3 signaling

et al. 2023

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Rheumatoid arthritis (RA) affects the joints and the endocrine system via persistent immune system activation. RA patients have a higher frequency of testicular dysfunction, impotence, and decreased libido. This investigation aimed to evaluate the efficacy of galantamine (GAL) on testicular injury secondary to RA. Rats were allocated into four groups: control, GAL (2 mg/kg/day, p.o), CFA (0.3 mg/kg, s.c), and CFA + GAL. Testicular injury indicators, such as testosterone level, sperm count, and gonadosomatic index, were evaluated. Inflammatory indicators, such as interleukin-6 (IL-6), p-Nuclear factor kappa B (NF-κB p65), and anti-inflammatory cytokine interleukin-10 (IL-10), were assessed. Cleaved caspase-3 expression was immunohistochemically investigated. Protein expressions of Janus kinase (JAK), signal transducers and activators of transcription (STAT3), and Suppressors of Cytokine Signaling 3 (SOCS3) were examined by Western blot analysis. Results show that serum testosterone, sperm count, and gonadosomatic index were increased significantly by GAL. Additionally, GAL significantly diminished testicular IL-6 while improved IL-10 expression relative to CFA group. Furthermore, GAL attenuated testicular histopathological abnormalities by CFA and downregulated cleaved caspase-3 and NF-κB p65 expressions. It also downregulated JAK/STAT3 cascade with SOCS3 upregulation. In conclusion, GAL has potential protective effects on testicular damage secondary to RA via counteracting testicular inflammation, apoptosis, and inhibiting IL-6/JAK/STAT3/SOCS3 signaling. Graphical abstract

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Galantamine in rheumatoid arthritis: A cross talk of parasympathetic and sympathetic system regulates synovium-derived microRNAs and related pathogenic pathways

Cited by 7 publications

References 62 publications

Investigation of Antiangiogenic and Apoptotic Effects of Galantamine Obtained From Leucojum Aestivum on McF-7 Breast Cancer Cell Line

Investigation of Antiangiogenic and Apoptotic Effects of Galantamine Obtained From Leucojum Aestivum on McF-7 Breast Cancer Cell Line

Neuroprotective Action of Polyphenols and Phenolic Compounds: An Overview

Galantamine mitigates testicular injury and disturbed spermatogenesis in adjuvant arthritic rats via modulating apoptosis, inflammatory signals, and IL-6/JAK/STAT3/SOCS3 signaling

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