Galangin (GG) combined with cisplatin (DDP) to suppress human lung cancer by inhibition of STAT3-regulated NF-κB and Bcl-2/Bax signaling pathways

Yu, Shuo; Gong, Lan; Li, Nianfeng; Pan, Yunlong; Zhang, Lun

doi:10.1016/j.biopha.2017.10.059

Cited by 79 publications

(52 citation statements)

References 42 publications

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“…Nature of the Apicomplexan parasites is to inhibit the apoptosis related mechanism via regulating anti-apoptosis factors to survive and grow inside the host cell [34]. E.tenella has capability to prompt anti-apoptosis factor Bcl-xL and nuclear transcriptional factor NF-κB to protect their cells and growing in its second generation schizonts and can be act as antagonists by enhancing the activity of NF-κB which is responsible for regulating numerous anti-apoptosis proteins such as Bcl-xL, Bcl-2 and Mcl-1 [35]. Previous studies reported that Probiotics and herbs revealed ameliorative effects on apoptosis by prompting pro-apoptosis proteins conversely inhibiting anti-apoptosis elements [36,37].…”

Section: Discussionmentioning

confidence: 99%

Effects of probiotics and Bidens pilosa on the performance and gut health of chicken during induced E. tenella infection

Memon

Yang

et al. 2020

Preprint

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Background Probiotics and Medicinal plants are widely used as an alternative to treat different kinds of the acute and chronic diseases in all over the world. Bidens pilosa is commonly used as foods and therapeutics against various pathogens including protozoan. In the present study probiotics and Bidens pilosa were used to explore the anticoccidial activity during experimental infection of Eimeria tenella ( E. tenella ) in chicken Methods One hundred and fifty one-day-old Chinese yellow breed chickens were divided into five equal groups. Group 1 was kept as control group (CG; un-treated, unchallenged). All other groups were inoculated with E. tenella sporulated oocysts on day 21 of age. Group 2 was kept as positive control (CPG; un-treated, challenged). Group 3 was treated with probiotics (PG) at the rate of 1g/kg of feed. Group 4 was treated with Bidens pilosa (BPG) at the dose of 0.5%/kg of feed. Group 5 was kept as probiotics + Bidens pilosa group (PG + BPG). All birds were weighed initially, day of infection and the final day of experiment. On day 5, 6, 7 and 8 post-infection (PI), bloody diarrhea was scored as normal (0) to sever (1, 2, 3 and 4), at the same time oocysts shedding was quantified by McMaster method. Gross caecal lesion scoring was checked at the final day of experiment. The apoptosis related gene expressions (Bcl-2, Bax, Caspase-3), anti-oxidant enzymatic expressions (CAT, SOD 1), Peptide transporter 1 (PepT 1), pro-inflammatory cytokines (IL-6, IL-8) and tight junction proteins expressions (CLDN 1, ZO 1) were checked by real time PCR. All related gene expressions average relative to β-actin was calculated by 2−ΔΔCt method. Results Birds treated with probiotics, Bidens pilosa and probiotics + Bidens pilosa slightly increased the body weight gain, significantly reduced oocysts shedding, destructive ratio of ceaca and mortality % than control positive group. CPG birds up-regulated the expressions of Bcl-2 while down-regulated the expressions of Bax and caspase-3 as compared to PG, BPG and PG + BPG groups. Moreover, Eimeria challenged chickens supplemented with probiotics, Bidens pilosa alone and combined showed higher anti-oxidant enzymatic activity than those of challenged un-treated chickens. Additionally, the expressions of pro-inflammatory cytokines and tight junction related proteins were also up-regulated by the treated challenged than un-treated challenged chickens. Conclusion Present results revealed that, feed supplementation of probiotics and Bides pilosa induced apoptosis, enhanced anti-oxidant enzymatic activity and pro-inflammatory cytokines as well as tight junction proteins and reduce mortality during E.tenella infection in chickens.

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Section: Discussionmentioning

confidence: 99%

Effects of probiotics and Bidens pilosa on the performance and gut health of chicken during induced E. tenella infection

Memon

Yang

et al. 2020

Preprint

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“…Decreasing the level of the anti‐apoptotic BCL‐2 protein and/or increasing the level of the pro‐apoptotic BAX protein plays an important role in disrupting mitochondrial homeostasis, which can lead to apoptotic cell death . Thus, the BAX/BCL‐2 ratio is a key regulator of apoptosis in lung cancer . Furthermore, caspase‐3, a member of the cysteine protease family, catalyzes crucial steps in apoptotic cell death .…”

Section: Discussionmentioning

confidence: 99%

“…27,28 Thus, the BAX/BCL-2 ratio is a key regulator of apoptosis in lung cancer. 29 Furthermore, caspase-3, a member of the cysteine protease family, catalyzes crucial steps in apoptotic cell death. 27 In this study, SCU increased the BAX/BCL-2 ratio and the amount of cleaved caspase-3 in A549 cells.…”

Section: Discussionmentioning

confidence: 99%

Scutellarin suppresses proliferation and promotes apoptosis in A549 lung adenocarcinoma cells via AKT/mTOR/4EBP1 and STAT3 pathways

Cao

Liu

et al. 2019

Thoracic Cancer

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Background Scutellarin (SCU), a flavonoid isolated from Erigeron breviscapus (Vant.) Hand.‐Mazz., increases autophagy and apoptosis in the adenocarcinoma A549 cell line, which is resistant to cisplatin. However, whether SCU alone has antitumor activity against non‐small cell lung cancer (NSCLC) is unknown. Methods Cell Counting Kit‐8, flow cytometry, colony formation, Hoechst 33258 staining, and Western blot analyses were used to examine the proliferation and apoptosis of A549 cells treated with SCU and the possible molecular mechanisms. Results The cell viability assay indicated that SCU markedly suppressed the proliferation of A549 cells in concentration and time‐dependent manners. SCU caused significant G0/G1 phase arrest and apoptosis, as evidenced by flow cytometric analyses, Hoechst 33258 staining, and Western blot analyses of cyclin D1, cyclin E, BCL‐2, cleaved‐caspase‐3, and BAX. Furthermore, SCU treatment reduced the level of pan‐AKT, phosphorylated (p)‐mTOR, mTOR, BCL‐XL, STAT3, and p‐STAT3, and increased the level of 4EBP1. Conclusions SCU can suppress proliferation and promote apoptosis in A549 cells through AKT/mTOR/4EBP1 and STAT3 pathways. This suggests that SCU may be developed into a promising antitumor agent for treating NSCLC.

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“…Many studies have shown that some important signaling pathways such as apoptotic play a key role in the occurrence and development of tumors [7][8][9]. Researchers have also found that the Bcl-2 protein as a major anti-apoptotic member of Bcl-2 family proteins is closely related to apoptotic pathway [10,11]. Tumor cells avoid apoptosis by overexpressing Bcl-2 protein, which is helpful for tumorigenesis and metastasis [12,13].…”

Section: Introductionmentioning

confidence: 99%

Dual-targeted cationic liposomes modified with hyaluronic acid and folic acid deliver siRNA Bcl-2 in the treatment of cervical cancer

Tong¹,

Wan²,

Yang³

et al. 2020

Preprint

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Backgroud: Gene therapy has attracted widespread attention as a potential method of treating some autoimmune diseases, genetic diseases and cancer. It is critical to discover a new nanocarrier for the effectiveness of gene therapy. Reportedly, the dual-targed nano-delivery system as a novel strategy could improve targeting efficiency and deliver more drugs or genes to tumor sites over current single-targed nano-delivery delivery system.Methods: We synthesized and characterized the hyaluronic acid (HA)-folic acid (FA) polymer, and then prepared the cationic liposomes by ultrasonic dispersion . The liposome was coated with HA-FA to obtain HA-FA-Lip nanoparticles. HA-FA-Lip formed complexes with siRNA Bcl-2 (siBcl-2) through electrostatic adsorption for delivering siBcl-2 to the tumor site.Results: HA-FA-Lip showed stronger tumor accumulation and better targeting efficiency than HA-lip. Meanwhile, HA-FA-Lip delivered more siBcl-2 to tumor cells and played a role in down-regulating Bcl protein levels, thus inducing obvious apoptosis.Conclusions: As a new type of dual-targeted drug delivery system, HA-FA-Lip was expected to be an effective nanocarrier with high transfection efficiency and good biocompatibility.

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Galangin (GG) combined with cisplatin (DDP) to suppress human lung cancer by inhibition of STAT3-regulated NF-κB and Bcl-2/Bax signaling pathways

Cited by 79 publications

References 42 publications

Effects of probiotics and Bidens pilosa on the performance and gut health of chicken during induced E. tenella infection

Effects of probiotics and Bidens pilosa on the performance and gut health of chicken during induced E. tenella infection

Scutellarin suppresses proliferation and promotes apoptosis in A549 lung adenocarcinoma cells via AKT/mTOR/4EBP1 and STAT3 pathways

Dual-targeted cationic liposomes modified with hyaluronic acid and folic acid deliver siRNA Bcl-2 in the treatment of cervical cancer

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