Galactose uptake by human platelets in vitro

Horne, McDonald K.; Hart, Jeffrey S.

doi:10.1016/0005-2736(86)90136-7

Cited by 3 publications

(1 citation statement)

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“…Previously we had identified two kinetically distinct platelet transporters for D-galactose [16]. Although CB inhibited both of these, the mechanism of inhibition appeared to be different for each.…”

Section: Abstract: Cytachalasin B Platelets Cytachalasin Bindingmentioning

confidence: 87%

Binding of cytochalasin B to platelets

Horne

1989

J of Cellular Biochemistry

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The binding of cytochalasin B (CB) to human platelets and to isolated platelet cytosol and membranes has been analyzed with [3H]CB. High- and low affinity classes of saturable binding sites were associated with intact platelets. Binding at very low concentrations of CB (i.e., high-affinity binding) was partially prevented by 100 mM D-galactose or D-glucose and to a much lesser extent by L-glucose. Binding to platelet cytosol also involved two classes of sites with affinities and capacities similar to those observed with the whole cells. None of this binding, however, was affected by 100 mM D-galactose. Saturable binding to platelet membranes occurred at sites with a uniform binding affinity. Approximately 52% of this binding was prevented by 1 M D-galactose and another 15% by cytochalasin E (CE). We hypothesize that binding in the cytosol is to monomeric (low-affinity) and polymerized (high-affinity) actin, whereas membrane binding (high-affinity only) occurs primarily at sites involved with galactose transport.

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Section: Abstract: Cytachalasin B Platelets Cytachalasin Bindingmentioning

confidence: 87%